Evaluation of Bacillus albus SMG-1 and B. safensis SMG-2 isolated from Saemangeum Lake as probiotics for aquaculture of white shrimp (Litopenaeus vannamei)

Kim, Soohwan; Jeon, Hyuncheol; Han, Hyon‐Sob; Hur, Jun Wook

doi:10.1016/j.aqrep.2021.100743

Cited by 14 publications

(18 citation statements)

References 70 publications

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“…Thus, bacteria of the genus Bacillus are autochthonous spore-forming inhabitants of soil and freshwater bodies. They can exist in a wide variety of temperatures in psychrophilic and thermophilic forms and play the role of primary colonizers in aquatic communities (Wu et al, 2017;Huang et al, 2018;Kim et al, 2021). A characteristic feature of these microorganisms is the ability to reduce nitrates in water (Rajakumar et al, 2008), use heavy metals to form spores, thereby reducing their concentration (Yin et al, 2018;Ramírez et al, 2019;Wang et al, 2020), and remove oil spills (Tanzadeh et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Chebarkul' Lake, which we took as an arbitrary control water body, demonstrated a community of microorganisms with a high potential for self-cleaning since, it was based on associations of genus Bacillus microorganisms (Bacillus spp., B. cereus, B. megaterium), whose distinctive feature is resistance to extreme conditions (including the low temperatures and low oxygenation in winter). Besides, they are natural destructors of toxic compounds (Alamri, 2012;Müller et al, 2015;Kim et al, 2021). Because of the high bacillus representation in the water body, Chebarkul' Lake had no indicators of fresh fecal contamination (coliform bacteria and enterococcus).…”

Section: Discussionmentioning

confidence: 99%

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Variability of the structure of winter microbial communities in Chelyabinsk lakes

et al. 2021

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Microorganisms form complex and dynamic communities that play a key role in the biogeochemical cycles of lakes. A high level of urbanization is currently a serious threat to bacterial communities and the ecosystem of freshwater bodies. To assess the contribution of anthropogenic load to variations in the structure of winter microbial communities in lakes, microorganisms of four water bodies of Chelyabinsk region were studied for the first time. We used cultural, chromatography-mass spectrometric, and modern methods of statistical data processing (particularly, multivariate exploratory analysis and canonical analysis of correspondences). The research showed that the composition of winter microbial communities in lakes Chebarkul’, Smolino, Pervoye, and Shershenevskoye Reservoir did not differ significantly between the main phyla of microorganisms. The dominant microorganisms were found to be of the Firmicutes phylum and Actinobacteria phylum. The structure of bacterial communities had special features depending on the characteristics of the water body and the sampling depths. Thus, in the lakes Smolino, Pervoye, and Shershenevskoye Reservoir, an important role was played by associations between microorganisms – indicators of fecal contamination: coliform bacteria and Enterococcus. On the contrary, in Chebarkul’ Lake, members of the genus Bacillus, which are natural bioremediators, formed stable winter associations. However, the differences between water bodies and sampling depths reflected 28.1% and 9.8% of the variability of the winter microbial communities, respectively. The largest contribution (about 60%) to the variability of the structure was made by intra-water processes, which determined the high heterogeneity of samples from different water areas. We assume that an important role in this variability was played by the high anthropogenic impact in a large industrial metropolis. In our opinion, this line of research is very promising for addressing key environmental issues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Variability of the structure of winter microbial communities in Chelyabinsk lakes

et al. 2021

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“…Cells were incubated 48 h at 25 • C, and bacterial species were identified using 16S rDNA sequencing. The microorganism genomes were sequenced using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Hampton, NY, USA) according to the manufacturer's instructions after PCR amplification using the 27F-CM (5 -AGA GTT TGA TCM TGG CTC AG-3 ) and 1492R (5 -TAC GGY TAC CTT GTT A-C GAC TT-3 ) primer pairs [37,38]. A BLAST similarity search of the 16S rRNA was conducted of the National Centre for Biotechnology Information (NCBI) database using BLASTN.…”

Section: Extraction Of Microorganisms From Saemangeum Reservoirmentioning

confidence: 99%

“…The two bacterial strains (probiotics) and carcasses of shrimp were freeze-dried for amino acid analysis [37]. Each sample (0.02 g) was hydrolyzed with 15 mL of 6 M HCl at 110 • C for 24 h. The samples were hydrolyzed in distilled water in a 50 mL flask, then evaporated and recovered in sodium citrate buffer (pH 2.2).…”

Section: Amino Acid Analysismentioning

confidence: 99%

“…In this study, two bacterial species, Salipiger thiooxidans and Exiguobacterium aestuarii, extracted and screened (fast-growing) from the Saemangeum Reservoir, the same environment as in the previous study [37], were selected and tested. This study aimed to assess the suitability of using S. thiooxidans or E. aestuarii in the rearing water of L. vannamei as a probiotic for this shrimp, by evaluating the growth performance, hemolymph parameters, and water quality.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Salipiger thiooxidans and Exiguobacterium aestuarii from the Saemangeum Reservoir as Potential Probiotics for Pacific White Shrimp (Litopenaeus vannamei)

et al. 2022

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In this study, two bacterial species, Salipiger thiooxidans and Exiguobacterium aestuarii, were extracted and screened from the Saemangeum Reservoir. This study examined these species’ suitability as a probiotic by confirming the effects of S. thiooxidans and E. aestuarii added to rearing water for L. vannamei. Three experimental groups were evaluated for 6 weeks: (1) a control group reared in natural (i.e., untreated) water (CON), (2) an experimental group in which S. thiooxidans was added to natural water (SMG-A), and (3) natural water inoculated with E. aestuarii (SMG-B). The SMG-B group inoculated with E. aestuarii showed significantly higher final body weight, weight gain, specific growth rates, and feed efficiency than the control group. The SMG-B group inoculated with E. aestuarii exhibited significantly higher levels of serum lysozyme, and ACP and ALP activity than the control and SMG-A groups. The SMG-A and SMG-B groups inoculated with probiotics showed significantly lower total ammonia nitrogen and nitrite than the control group. Our findings suggest that S. thiooxidans and E. aestuarii extracted from the Saemangeum Reservoir can improve the water quality of aquaculture water, and, in particular, E. aestuarii is a potential probiotic for L. vannamei.

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Algae Species of Industrial, Environmental, and Food Importance

Khalid,

Yousaf,

Amin

et al. 2024

Cyanobacteria Biotechnology

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Evaluation of Bacillus albus SMG-1 and B. safensis SMG-2 isolated from Saemangeum Lake as probiotics for aquaculture of white shrimp (Litopenaeus vannamei)

Cited by 14 publications

References 70 publications

Variability of the structure of winter microbial communities in Chelyabinsk lakes

Variability of the structure of winter microbial communities in Chelyabinsk lakes

Evaluation of Salipiger thiooxidans and Exiguobacterium aestuarii from the Saemangeum Reservoir as Potential Probiotics for Pacific White Shrimp (Litopenaeus vannamei)

Algae Species of Industrial, Environmental, and Food Importance

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