Evaluation of Bacillus-fortified organic fertilizer for controlling tobacco bacterial wilt in greenhouse and field experiments

Yuan, Saifei; Wang, Lili; Wu, Kai; Shi, Jingying; Wang, Maosheng; Yang, X. B.; Shen, Qirong; Shen, Biao

doi:10.1016/j.apsoil.2013.11.004

Cited by 85 publications

(51 citation statements)

References 45 publications

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“…However, opposite results were observed for fungi. These results agree with previous studies that biofertilizer application can increase soil bacterial diversity in the rhizosphere of banana (Shen et al 2015), cucumber (Qiu et al 2012), and tobacco (Yuan et al 2014) and decrease soil fungal diversity in the rhizosphere of cucumber , cotton (Luo et al 2010), and watermelon (Zhao et al 2011), as shown by the use of PCR-DGGE technique.…”

Section: Microbial Diversitysupporting

confidence: 94%

Rhizosphere microbial community manipulated by 2 years of consecutive biofertilizer application associated with banana Fusarium wilt disease suppression

et al. 2015

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In our previous work, applying biofertilizer containing Bacillus amyloliquefaciens strain NJN-6 to a banana orchard infected by a serious Fusarium wilt disease over two consecutive years effectively controlled this soil-borne disease. In this study, deep pyrosequencing of 16S ribosomal RNA (rRNA) genes and internal transcribed spacer (ITS) sequences was performed to investigate how the composition of rhizosphere microbial community responded to the application of biofertilizer (BIO), pig manure compost (PM), and chemical fertilizer (CF) and to explore the potential correlation between the microbial community composition and the Fusarium wilt disease. A total of 104,201 bacterial 16S rRNA genes and 154,953 fungal ITS sequence reads were obtained after basic quality control, and Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, and Ascomycota were the most abundant bacterial and fungal phyla across all samples. Compared with the PM and CF control, the alpha diversity of bacteria significantly (P<0.05) increased, whereas the value of the fungi was significantly (P<0.05) reduced following two consecutive years of biofertilizer application. Moreover, the abundance of Acidobacteria (Gp1 and Gp3), Firmicutes, Leptosphaeria, and Phaeosphaeriopsis was significantly (P < 0.05) increased, while the abundance of Proteobacteria and Ascomycota was significantly (P<0.05) decreased in the BIO treatment. Furthermore, the abundance of Fusarium, a causal pathogen for Fusarium wilt disease, was significantly (P<0.05) reduced in the BIO treatment compared with the CF control and was slightly reduced (not significant) compared with the PM control. Interestingly, the disease incidence was negatively correlated with the enriched taxa of Acidobacteria (Gp1 and Gp3) and Firmicutes, Leptosphaeria, and Phaeosphaeriopsis but positively correlated with abundance of Proteobacteria, Ascomycota, Fusarium, Cylindrocarpon, Gymnascella, Monographella, Pochonia, and Sakaguchia taxa. The results from this study suggest that 2 years of biofertilizer application manipulated the composition of rhizosphere microbial community and induced the Fusarium suppression by increasing bacterial diversity and potentially stimulating microbial consortia taxa, such as Acidobacteria (Gp1 and Gp3), Firmicutes, Leptosphaeria, and Phaeosphaeriopsis.

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Section: Microbial Diversitysupporting

confidence: 94%

Rhizosphere microbial community manipulated by 2 years of consecutive biofertilizer application associated with banana Fusarium wilt disease suppression

et al. 2015

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“…Additionally, the relative abundances of genera identified as Bacillus sp., Arthrobacter sp. and Sphingomonas sp., are now being used as worldwide bacterial antagonists against R. solanacearum (Li et al 2005;Hu et al 2007;Huang et al 2011;Yuan et al 2014). All of the three genera were increased in L-CF and L-OM treatments (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…An abundance of beneficial soil organisms could suppress pathogens and diseases, improve nutrient availability, promote plant growth, and thus increased the crop yield (Ji et al 2008;Ramesh et al 2009;Liu et al 2012;Yuan et al 2014). Streptomyces sp.…”

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confidence: 99%

Changes in the abundance and structure of bacterial communities under long-term fertilization treatments in a peanut monocropping system

Liu

Wang

et al. 2015

Plant Soil

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Background and aims Peanut yield and quality are seriously compromised by continuous monoculturing in the red soil region of southern China. Monoculturing can cause soil degradation and an increase in soil-borne diseases. This research aimed to investigate the influence of long-term peanut monocropping and different fertilization treatments on peanut growth, soil physical and chemical properties and soil microbial community. Methods A long-term fertilization experiment established in 1996 was utilized to examine the effect of various fertilization treatments including chemical and organic fertilizers treatments. Deep 16S rRNA gene pyrosequencing highlighted changes in the abundance and structure of bacterial communities, especially of the pathogenic and beneficial bacterial communities in long term chemical fertilizer treatment in comparison to the organic manure treatment. Results Chemical fertilizer treatment causes a shift in bacterial community structure and decrease in diversity under the long-term monocropping in comparison to organic fertilizer. The abundance of the bacterial pathogen Ralstonia solanacearum, a causative agent of peanut wilt, was found to be associated with a loss of community diversity and loss of the peanut yield. Conclusions The organic fertilizers more effectively increase microbial diversity in the soil and changed the community structure. Long-term use of the chemical fertilizer leads to a decrease in microbial diversity of the soil and an increase in R. solanacearum with associated increase of peanut wilt. The potential decrease in diversity and competition between the bacterial community and the pathogen may be a contributing factor to increased disease during long-term chemical fertilizer use.

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“…It is known that crop residues are a valuable source of organic matter for most agricultural soils; they can improve the soil quality and productivity through favorable effects on soil properties such as plant available water holding capacity, cation exchange capacity, and the stimulation of beneficial microorganisms (Saroa and Lal, 2003). It is known that the occurrence of beneficial soil organisms could suppress pathogens and diseases, improve nutrient availability, promote plant growth, and thus increase the crop yield (Yuan et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Strawberry monocropping: Impacts on fruit yield and soil microorganisms

Lovaisa

Guerrero-Molina

Delaporte-Quintana

et al. 2017

J. Soil Sci. Plant Nutr.

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The objective of this work was to explore the impacts of intensive strawberry monocropping during five years on the fruit-yield and on the microbial soil activity and composition. Field trials were performed in two plots: P1(a soil with five years of consecutive strawberry cropping), and P2 (a soil with just one year of strawberry cropping). Fruit-yield was quantified; total microorganisms and four functional groups (cellulolytics, nitrogen-fixers, phosphate solubilizers, and siderophores producers) were quantified, isolated and characterized in both plots.Total microbial activity was assessed by the hydrolysis of fluorescein-diacetate and soil respiration methods. As results, in P1, a 51 % decrease in fruit-yield was observed, while in P2 it was closer to the yield obtained in P1 during the first and second year of cultivation. Total microbial number and activity were 23 % and 70 % lower in P1 than in P2 at the end of the cropping, respectively. In general, the quantity of total culturable microorganisms and the functional groups analyzed were significantly higher in P2 than in P1 (p< 0.05).The most prevailing bacteria putatively identified were Cellulosimicrobium cellulans, Paenibacillus sp., Azospirillum brasilense, and Burkholderia sp. According to our results, the intensive cultivation of strawberry for several years in the same field exerted a negative impact on the soil quality, affecting the native microbial population, which might be linked to the fruit yield decline.

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Evaluation of Bacillus-fortified organic fertilizer for controlling tobacco bacterial wilt in greenhouse and field experiments

Cited by 85 publications

References 45 publications

Rhizosphere microbial community manipulated by 2 years of consecutive biofertilizer application associated with banana Fusarium wilt disease suppression

Rhizosphere microbial community manipulated by 2 years of consecutive biofertilizer application associated with banana Fusarium wilt disease suppression

Changes in the abundance and structure of bacterial communities under long-term fertilization treatments in a peanut monocropping system

Strawberry monocropping: Impacts on fruit yield and soil microorganisms

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