Evaluation of Bacillus subtilis as a probiotic to Indian major carp Labeo rohita (Ham.)

Kumar, Rajesh; Mukherjee, Subhas; Prasad, K. Pani; Pal, A. K.

doi:10.1111/j.1365-2109.2006.01551.x

Cited by 186 publications

(138 citation statements)

References 17 publications

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“…Above probiotics and plant extracts were checked for their antimicrobial activity against the fish bacterial pathogens using agar well diffusion method (Gram et al, 2004). The culture medium was poisoned with pathogenic bacterial culture with concentration 1.64 × 10 10 cfu/ml.…”

Section: In Vitro Antagonistic Testmentioning

confidence: 99%

Effect of probiotics, antibiotics and herbal extracts against fish bacterial pathogens

Pannu

Dahiya²,

Sabhlok³

et al. 2024

Ecotoxicol. Environ. Contam.

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In this study the effect of probiotic preparations and plant extract was investigated against eleven fish bacterial pathogens viz. Aeromonas hydrophila, Cellobiococcus sp., Enterobacter aerogenes, E. cloacae, Klebsiella pneumoniae, Salmonella sp., Shigella sp., Streptobacillus sp., Streptococcus sp., Pseudomonas fluorescens and Staphylococcus aureus. All these pathogens were found to be resistant for antibiotics viz. nitrofurantoin, amoxycillin, bacitracin, cephalothin, erythromycin, novobiocin, vancomycin , amphicillin, oxacillin and colistin. In vitro antagonism test of the probiotics was performed by using well diffusion method. In case of probiotic Aquapro TM , effective zone of inhibition of 2.433 cm was observed for P. fluorescens and K. pneumoniae. The extracts of Azadirachta indica, Aloe barbadensis, Withania somnifera and Momordica charantia were studied in vitro alone as well as in combination with probiotic (Lactobacillus sporogenes). The zone of inhibition observed in mixed sample was less as compared to individual sample. Among the four plants extract, the W. somnifera extract was found to be the most effective and it leads to maximum inhibition (1.1 cm) recorded for E. aerogenes.

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Section: In Vitro Antagonistic Testmentioning

confidence: 99%

Effect of probiotics, antibiotics and herbal extracts against fish bacterial pathogens

Pannu

Dahiya²,

Sabhlok³

et al. 2024

Ecotoxicol. Environ. Contam.

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show abstract

“…The significant increases in WBCs counts in fish fed with the nigella or combination diets may be due to the activation of the hemopoietic tissues either by the black cumin [26] or B. subtilis [27]. Several types of leukocytes participate in the cellular immune response, including lymphocytes, monocytes, granulocytes (neutrophils, eosinophils and basophils), and cytotoxic cells [28].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulation of Nile Tilapia, Oreochromis niloticus, by Nigella sativa and Bacillus subtilis

Elkamel¹,

Mosaad²

2012

J Aquac Res Development

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The aim of this study is to investigate the modulation of the immune system of Nile tilapia, Oreochromis niloticus, by the black cumin seeds, Nigella sativa, or Bacillus subtilis PB6 (CloSTAT) as feed additives. Four diet regimes, a basic (control), CloSTAT, nigella, or combination of the black cumin seeds and CloSTAT were formulated and used to feed fish for 30 successive days. Half of the fish were used to investigate some of the immune parameters as serum globulins, white blood cell counts, and phagocytic activities and phagocytic indices. The other half of fish was subjected to infection challenge with Aeromonas hydrophila to investigate the disease resistance ability of the fish received the feeding additives. Results showed that the serum globulins have significantly increased in fish received the combination diet, while white blood cells have significantly increased in fish fed with nigella or combination ration. Phagocytic activities and indices of the fish fed the combination ration were significantly higher than those of the control groups. The mortality rate of fish fed with nigella or combination ration and challenged with A. hydrophila were significantly lower than those that received the basic diet. The current study clearly demonstrated that black cumin, CloSTAT or both can be used to modulate the immune system of Nile tilapia to the favour of resistance to diseases. replicates into sets and groups. Each replicate included two sets, where one set was used for evaluating the immune blood parameters of fish (blood set), while the other set was used for the challenge experiment (challenge set). Each blood set had four equal experimental groups that represent the 4 nutritional treatments (corresponding to the 4 diet variants). Thus, a control group received the basic diet, the second group received the CloSTAT diet, the third group received the nigella diet, while the fourth group received the combination diet. While each challenge set had the same four groups as the blood set and assigned as challenge groups. Each challenge set had 2 additional groups assigned as challenge control groups that received the basic diet and remained unchallenged as a control for the challenge experiment. Each group had 7 fish and was fed ad libitum with only one diet variant twice daily for 30 successive days. Immunomodulation of NileA total of 30 glass aquaria of 100 L capacity were used to conduct this study. Throughout the experiment, the temperature was maintained at 24°C and the dissolved oxygen level at 6.0-7.0 mg/L, while the pH levels were 7.2-7.5. Haematological studiesBy the end of the feeding experiment, fish of all groups of the blood sets were anesthetized using tricaine methanesulfonate (MS-222, Argent Chemical Labs, Redmond, Washington, USA). Blood samples were collected from the caudal vein of fish and were divided into two portions. One part was collected with 3% sodium citrate and used for white blood cells count or mixed with Candida albicans culture to assess phagocytic activity. The second po...

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“…Kumar and colleagues [34] observed higher survival rate of carp Labeo rohita fed Bacillus subtilis, submitted to intraperitoneal injection with Aeromonas hydrophila.…”

Section: Immune Systemmentioning

confidence: 97%

Use of Probiotics in Aquaculture

Azevedo¹,

Braga²

2012

Probiotic in Animals

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Evaluation of Bacillus subtilis as a probiotic to Indian major carp Labeo rohita (Ham.)

Cited by 186 publications

References 17 publications

Effect of probiotics, antibiotics and herbal extracts against fish bacterial pathogens

Effect of probiotics, antibiotics and herbal extracts against fish bacterial pathogens

Immunomodulation of Nile Tilapia, Oreochromis niloticus, by Nigella sativa and Bacillus subtilis

Use of Probiotics in Aquaculture

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