Evaluation of bacterial adherence of clinical isolates of<i>Staphylococcus sp.</i>using a competitive model

Martínez-Pérez, Marta; Pérez‐Jorge, Concepción; Lozano, Daniel; Portal‐Núñez, Sergio; Pérez‐Tanoira, Ramón; Conde, A.; Arenas, Miguel; Hernández-López, Juan Manuel; Damborenea, J. de; Gómez‐Barrena, Enrique; Esbrit, Pedro; Esteban, Jaime

doi:10.1302/2046-3758.65.bjr-2016-0226.r2

Cited by 27 publications

(21 citation statements)

References 43 publications

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“…Following this hypothesis, some works are reported in the literature involving the bone-dedicated materials and combining, for example, the human osteosarcoma (U2OS or K12) cells and Staphylococcus epidermidis or Staphylococcus aureus [37], the mouse embryonic pre-fibroblasts (MC3T3-E1), and Staphylococcus epidermidis [38], but the results are not easy to interpret. In fact, most of the results coming from the present literature suggest that the outcome of the study depends mainly by the bacterial inoculum concentration used at the start of the competition [37,[39][40][41]; in fact, considering a concentration range from 1 × 10 2 to 1 × 10 5 bacteria/specimen, only the lower inoculum led to significant results between antibacterial and control surfaces. These results logically correlate the improvement of the aseptic surgery conditions and pharmacological prophylaxis that can dramatically lower the ratio of the infection with the success of implantology due to the intrinsic ability of the biomaterials to counteract the low rate of infections.…”

Section: Discussionmentioning

confidence: 83%

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

et al. 2020

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Nowadays, there is a large amount of research aimed at improving the multifunctional behavior of the biomaterials for bone contact, including the concomitant ability to induce apatite formation (bioactivity), fast and effective osteoblasts colonization, and antibacterial activity. The aim of this study is to develop antibacterial and bioactive surfaces (Ti6Al4V alloy and a silica-based bioactive glass) by chemical doping with strontium and/or silver ions. The surfaces were characterized by Scanning Electron Microscopy equipped with Energy Dispersive X ray Spectroscopy (SEM-EDS), X-ray photoelectron spectroscopy (XPS), and Transmission Electron Microscopy (TEM). To better focus on the cells–bacteria competition for the implant surface, in addition to the standard assays for the evaluation of the bacteria adhesion (ISO22196) and for single-cell cultures or biofilm formation, an innovative set of co-cultures of cells and bacteria is here proposed to simulate a competitive surface colonization. The results suggest that all the bioactive tested materials were cytocompatible toward the bone progenitor cells representative for the self-healing process, and that the doped ones were effective in reducing the surface colonization from a pathogenic drug-resistant strain of Staphylococcus aureus. The co-cultures experiments demonstrated that the doped surfaces were able to protect the adhered osteoblasts from the bacteria colonization as well as prevent the infection prior to the surface colonization by the osteoblasts.

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Section: Discussionmentioning

confidence: 83%

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

et al. 2020

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“…(i) Cell adhesion. Cell adhesion was evaluated using a previously described methodology (97). Briefly, the Ti-6Al-4V samples were placed into six-well plates before MCT3T3-E1 cells were seeded at a density of 10 5 cells/ml in 4 ml of alpha minimum essential medium with 10% fetal bovine serum, followed by incubation for 6 h at 37°C in 5% CO 2 under static conditions.…”

Section: Methodsmentioning

confidence: 99%

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Aguilera-Correa

Mediero

Conesa-Buendía

et al. 2019

Appl Environ Microbiol

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Joint prosthesis failure is mainly related to aseptic loosening and prosthetic joint infections, both of which are associated with high morbidity and substantial costs for patients and health systems. The development of a biomaterial that is capable of stimulating bone growth while minimizing bacterial adhesion would reduce the incidence of prosthetic failure. We report antibacterial and osteostimulatory effects in a novel fluorine-phosphorus (F-P)-doped TiO2 oxide film grown on Ti-6Al-4V alloy with a nanostructure of bottle-shaped nanotubes (bNT) using five bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia) and MCT3T3-E1 osteoblastic cells. The interaction between the bacteria and bNT Ti-6Al-4V was complex, as the adhesion of four bacterial species decreased (two staphylococcus species, E. coli, and S. maltophilia), and the viability of staphylococci and S. maltophilia also decreased because of the aluminum (Al) released by bNT Ti-6Al-4V. This released Al can be recruited by the bacteria through siderophores and was retained only by the Gram-negative bacteria tested. P. aeruginosa showed higher adhesion on bNT Ti-6Al-4V than on chemically polished (CP) samples of Ti-6Al-4V alloy and an ability to mobilize Al from bNT Ti-6Al-4V. The cell adhesion and proliferation of MCT3T3-E1 osteoblastic cells significantly increased at 48 and 168 h, as did the matrix mineralization of these cells and the gene expression levels of three of the most important markers related to bone differentiation. According to our results, the bNT Ti-6Al-4V alloy could have clinical application, preventing infection and stimulating bone growth and thus preventing the two main causes of joint prosthesis failure. IMPORTANCE This work evaluates F-P-doped bNT Ti-6Al-4V from microbiological and cellular approaches. The bacterial results highlight that the antibacterial ability of bNT Ti-6Al-4V is the result of a combination of antiadhesive and bactericidal effects exerted by Al released from the alloy. The cell results highlight that F-P bNT Ti-6Al-4V alloy increases osseointegration due to modification of the chemical composition of the alloy resulting from P incorporation and not due to the nanostructure, as reported previously. A key finding was the detection of Al release from inside the bNT Ti-6Al-4V nanostructures, a result of the nanostructure growth during the anodizing process that is in part responsible for its bactericidal effect.

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“…Therefore, local antibiotic therapy was proposed as an alternative and/or adjuvant to systemic prophylaxis or treatment, preventing systemic toxicity and favoring drug release directly within the implant site (Zhao et al, 2009). Additionally, if the infection probability is reduced, the osteointegration would be improved (Gristina, 1987;Zhao et al, 2014;Martinez-Perez et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A New Antibiotic-Loaded Sol-Gel Can Prevent Bacterial Prosthetic Joint Infection: From in vitro Studies to an in vivo Model

et al. 2020

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The aim of this study was to evaluate the effect of a moxifloxacin-loaded organicinorganic sol-gel with different antibiotic concentration in the in vitro biofilm development and treatment against Staphylococcus aureus, S. epidermidis, and Escherichia coli, cytotoxicity and cell proliferation of MC3T3-E1 osteoblasts; and its efficacy in preventing the prosthetic joint infection (PJI) caused by clinical strains of S. aureus and E. coli using an in vivo murine model. Three bacterial strains, S. epidermidis ATCC 35984, S. aureus 15981, and, E. coli ATCC 25922, were used for microbiological studies. Biofilm formation was induced using tryptic-soy supplemented with glucose for 24 h, and then, adhered and planktonic bacteria were estimated using drop plate method and absorbance, respectively. A 24-h-mature biofilm of each species growth in a 96well plate was treated for 24 h using a MBECTM biofilm Incubator lid with pegs coated with the different types of sol-gel, after incubation, biofilm viability was estimated using alamrBlue. MC3T3-E1 cellular cytotoxicity and proliferation were evaluated using CytoTox 96 Non-Radioactive Cytotoxicity Assay and alamarBlue, respectively. The microbiological studies showed that sol-gel coatings inhibited the biofilm development and treated to a mature biofilm of three evaluated bacterial species. The cell studies showed that the sol-gel both with and without moxifloxacin were non-cytotoxic and that cell proliferation was inversely proportional to the antibiotic concentration containing by sol-gel. In the in vivo study, mice weight increased over time, except in the E. coliinfected group without coating. The most frequent symptoms associated with infection were limping and piloerection; these symptoms were more frequent in infected groups

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Evaluation of bacterial adherence of clinical isolates ofStaphylococcus sp.using a competitive model

Cited by 27 publications

References 43 publications

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

A New Antibiotic-Loaded Sol-Gel Can Prevent Bacterial Prosthetic Joint Infection: From in vitro Studies to an in vivo Model

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