Evaluation of Bacterial Contamination in Goat Milk Powder Using PacBio Single Molecule Real-Time Sequencing and Droplet Digital PCR

Ma, Hongbing; Li, Jing; Xi, Xiaoxia; Xu, Haiyan; Laga, Wuri; Bian, Yanfei; Yu, Zhou; Min, Ren; Duo, Lana; Sun, Yaru; Sun, Zhihong; Sun, Tiansong; Menghe, Bilige

doi:10.4315/0362-028x.jfp-17-535

Cited by 4 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, infant formula in the Liaoning province (42%) was contaminated with B. cereus more seriously than that in Yunnan province (12%) ( Table 1 ). The regular rates of B. cereus in other cities or provinces, such as Beijing, Liaoning, Gansu, Yunnan, northeast China, were found to be 30%, 27%, 19%, 10% and 16%, respectively [ 13 , 75 , 76 , 77 ]. Among these data, Yunnan province and the Northeast China indeed had a relatively low prevalence of B. cereus in powdered infant formula (PIF).…”

Section: Prevalence Of Bacillus Cereus From Daimentioning

confidence: 99%

Characterization of Bacillus cereus in Dairy Products in China

Liu

et al. 2020

Toxins

View full text Add to dashboard Cite

Bacillus cereus is a common and ubiquitous foodborne pathogen with an increasing prevalence rate in dairy products in China. High and unmet demands for such products, particularly milk, raise the risk of B. cereus associated contamination. The presence of B. cereus and its virulence factors in dairy products may cause food poisoning and other illnesses. Thus, this review first summarizes the epidemiological characteristics and analytical assays of B. cereus from dairy products in China, providing insights into the implementation of intervention strategies. In addition, the recent achievements on the cytotoxicity and mechanisms of B. cereus are also presented to shed light on the therapeutic options for B. cereus associated infections.

show abstract

Section: Prevalence Of Bacillus Cereus From Daimentioning

confidence: 99%

Characterization of Bacillus cereus in Dairy Products in China

Liu

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

“…As evidenced by a number of studies, raw milk, dairy products and dairy farm environments are a common source of toxigenic B. cereus [4,5,6,12,13,15]. Simultaneously, psychrotrophic B. cereus strains capable of growing at temperatures below 8 °C can also be isolated [16].…”

Section: Introductionmentioning

confidence: 99%

Goat Colostrum—Source of Toxigenic Bacillus Cereus

et al. 2019

View full text Add to dashboard Cite

The aim of this study was to evaluate the toxigenic potential of Bacillus cereus strains isolated from frozen goat colostrum. Of the 50 phenotypically suspected B. cereus isolates, 39 (78.0 %) were confirmed as B. cereus by the polymerase chain reaction (PCR) method based on the gyrB gene detection. In these isolates, genes encoding the production of haemolysin BL (Hbl), a complex of non-haemolytic enterotoxins (Nhe) and emetic toxin were detected by the PCR method. In 36 (92.3 %) confirmed B. cereus isolates, genes encoding at least one type of toxins of interest were detected. In all toxigenic isolates, we found the presence of genes for Nhe production, and in 16 (41.0 %) of the isolates, genes encoding both Nhe and haemolysin BL were shown. Eight (20.5 %) of the emetic strains of B. cereus were identified. The emetic toxin production gene was always detected simultaneously with genes encoding non-haemolytic enterotoxin production. The ability to produce BL haemolysin and non-haemolytic enterotoxins were confirmed by the immunochromatographic method. In summary, goat colostrum can be a significant source of toxigenic strains of B. cereus.

show abstract

Bacillus cereus food intoxication and toxicoinfection

Jovanović

Ornelis

Madder

et al. 2021

Comp Rev Food Sci Food Safe

127

View full text Add to dashboard Cite

Bacillus cereus is one of the leading etiological agents of toxin‐induced foodborne diseases. Its omnipresence in different environments, spore formation, and its ability to adapt to varying conditions and produce harmful toxins make this pathogen a health hazard that should not be underestimated. Food poisoning by B. cereus can manifest itself as an emetic or diarrheal syndrome. The former is caused by the release of the potent peptide toxin cereulide, whereas the latter is the result of proteinaceous enterotoxins (e.g., hemolysin BL, nonhemolytic enterotoxin, and cytotoxin K). The final harmful effect is not only toxin and strain dependent, but is also affected by the stress responses, accessory virulence factors, and phenotypic properties under extrinsic, intrinsic, and explicit food conditions and host‐related environment. Infamous portrait of B. cereus as a foodborne pathogen, as well as a causative agent of nongastrointestinal infections and even nosocomial complications, has inspired vast volumes of multidisciplinary research in food and clinical domains. As a result, extensive original data became available asking for a new, both broad and deep, multifaceted look into the current state‐of‐the art regarding the role of B. cereus in food safety. In this review, we first provide an overview of the latest knowledge on B. cereus toxins and accessory virulence factors. Second, we describe the novel taxonomy and some of the most pertinent phenotypic characteristics of B. cereus related to food safety. We link these aspects to toxin production, overall pathogenesis, and interactions with its human host. Then we reflect on the prevalence of different toxinotypes in foods opening the scene for epidemiological aspects of B. cereus foodborne diseases and methods available to prevent food poisoning including overview of the different available methods to detect B. cereus and its toxins.

show abstract

Evaluation of Bacterial Contamination in Goat Milk Powder Using PacBio Single Molecule Real-Time Sequencing and Droplet Digital PCR

Cited by 4 publications

References 43 publications

Characterization of Bacillus cereus in Dairy Products in China

Characterization of Bacillus cereus in Dairy Products in China

Goat Colostrum—Source of Toxigenic Bacillus Cereus

Bacillus cereus food intoxication and toxicoinfection

Contact Info

Product

Resources

About