2021
DOI: 10.1186/s12934-021-01670-8
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Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

Abstract: Hydroxycinnamic acids such as p-coumaric acid (CA) are chemically linked to lignin in grassy biomass with fairly labile ester bonds and therefore represent a straightforward opportunity to extract and valorize lignin components. In this work, we investigated the enzymatic conversion of CA extracted from lignocellulose to 4-vinylphenol (4VP) by expressing a microbial phenolic acid decarboxylase in Corynebacterium glutamicum, Escherichia coli, and Bacillus subtilis. The performance of the recombinant strains was… Show more

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Cited by 19 publications
(12 citation statements)
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“…Significant advancements have been achieved in the biological depolymerization of lignin macromolecules, the metabolism of aromatic derivatives, and the bioproduction of 4VPs. 18,19,90 However, the substrate specificity and catalytic activity of enzymes, as well as the compatibility of chassis bacteria with heterologous genes, and their tolerance to aromatic substrates and products, remain significant challenges in the biological valorization of lignin for 4VPs. Fortunately, advanced strategies are currently available that provide effective solutions to overcome these obstacles.…”
Section: Emerging Cutting-edge Technologies Promoted Biological Ligni...mentioning
confidence: 99%
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“…Significant advancements have been achieved in the biological depolymerization of lignin macromolecules, the metabolism of aromatic derivatives, and the bioproduction of 4VPs. 18,19,90 However, the substrate specificity and catalytic activity of enzymes, as well as the compatibility of chassis bacteria with heterologous genes, and their tolerance to aromatic substrates and products, remain significant challenges in the biological valorization of lignin for 4VPs. Fortunately, advanced strategies are currently available that provide effective solutions to overcome these obstacles.…”
Section: Emerging Cutting-edge Technologies Promoted Biological Ligni...mentioning
confidence: 99%
“…This microbial cell factory allowed a maximum conversion of lignin-derived aromatic precursors to 4VP with a titer of 187 g L −1 in a biphasic system. 19 Synthetic biology has also been applied to enhance the synthesis and mass transfer of the pathway intermediate, namely tyrosine, to facilitate the de novo biosynthesis of 4VP. 106 The endogenous toxin gene hipA in E. coli was utilized as a selection element, with its expression controlled by the aroP promoter.…”
Section: Synthetic Biology Technologies Boosted the Construction Of L...mentioning
confidence: 99%
“…( R )‐Mandelic acid was produced from glycerol in E. coli , and a biphasic system using n‐ hexadecane increased the titer threefold by reducing the toxicity of the intermediates styrene and ( S )‐styrene oxide (Lukito et al, 2019). A high titer of 4‐vinylphenol was produced from p ‐coumaric acid in C. glutamicum expressing phenolic acid decarboxylase ( PAD ) and combined with solvent extraction (Rodriguez et al, 2021). Styrene was also produced from glucose in E. coli by the expression of PAL and ferulic acid decarboxylase ( FDC ), and combined with solvent extraction and/or gas stripping (K. Lee et al, 2019; Liang et al, 2020).…”
Section: Bioprocess Concepts For Phenylpropanoid Productionmentioning
confidence: 99%
“…The development of robust hosts which can tolerate high concentrations of phenylpropanoids and extreme process conditions is also required for whole‐cell biocatalysis to be economical, especially when co‐factor regeneration is required. When E. coli, C. glutamicum , and Bacillus subtilis were compared as PAD whole‐cell biocatalysts, C. glutamicum produced the highest titer of 4‐vinylphenol (Rodriguez et al, 2021). C. glutamicum was also successfully used as a PAL whole‐cell biocatalyst for the production of cinnamic acid, where it remained catalytically active for 12 h at alkaline pH and elevated temperature (Son et al, 2021).…”
Section: Bioprocess Concepts For Phenylpropanoid Productionmentioning
confidence: 99%
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