Evaluation of bactericidal and anti-biofilm properties of a novel surface-active organosilane biocide against healthcare associated pathogens and Pseudomonas aeruginosa biolfilm

Abstract

Healthcare acquired infections (HAI) pose a great threat in hospital settings and environmental contamination can be attributed to the spread of these. De-contamination and, significantly, prevention of re-contamination of the environment could help in preventing/reducing this threat. Goldshield (GS5) is a novel organosilane biocide marketed as a single application product with residual biocidal activity. We tested the hypothesis that GS5 could provide longer-term residual antimicrobial activity than existing disinfectants once applied to surfaces. Thus, the residual bactericidal properties of GS5, Actichlor and Distel against repeated challenge with Staphylococcus aureus ATCC43300 were tested, and showed that GS5 alone exhibited longer-term bactericidal activity for up to 6 days on 316I stainless steel surfaces. Having established efficacy against S. aureus, we tested GS5 against common healthcare acquired pathogens, and demonstrated that, on average, a 1 log10 bactericidal effect was exhibited by GS5 treated surfaces, although biocidal activity varied depending upon the surface type and the species of bacteria. The ability of GS5 to prevent Pseudomonas aeruginosa biofilm formation was measured in standard microtitre plate assays, where it had no significant effect on either biofilm formation or development. Taken together the data suggests that GS5 treatment of surfaces may be a useful means to reducing bacterial contamination in the context of infection control practices.

<p>Design and characterization of a new hybrid peptide from LL-37 and BMAP-27</p>

“…It was tested against MR S. aureus ATCC 33951 and MDR P. aeruginosa ATCC BAA-2114. These bacteria are known as biofilm formers 57,58. The MBEC values were two-fold and six-fold higher than the MIC values against the planktonic form of the aforementioned bacterial strains, respectively (Table 4).…”

Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility

“…What is clear is that the novel filamentous fungal isolate described here is a strong biofilm former; additionally, this study highlights the importance of testing antimicrobial surfaces with microbes other than the classic bacterial biofilm formers, Pseudomonas aeruginosa and Staphylococcus aureus. The antimicrobial compound used in this study was selected because it demonstrated substantial biocidal activity against both of these classic bacterial biofilm formers [25]; however, our study here indicates that when filamentous fungus is grown in the presence of the coating, the biofilm is actually larger (Figure 2). The reason for this enlarged biofilm on the coated surfaces as compared to the uncoated surfaces is not known, and further testing with microorganisms under different growth conditions could help to explain this finding.…”

“…Commercially available and patented organosilane, a surface-penetrating compound, was used as an antimicrobial coating on the tested surfaces. The active ingredient in the antimicrobial compound tested is 3-(trihydroxysilyl) propyldimethyloctadecyl ammonium chloride and previously assessed to control bacterial biofilm formation [25]. In the presence of hydroxyl groups at the surface of the glass, minerals, or metals (e.g., aluminum, steel), silanols formed a stable Si bond.…”

Effective antiviral coatings for deactivating SARS-CoV-2 virus on N95 respirator masks or filters

“…The technology is currently registered with the US Environmental Protection Agency (85556–1 and 85556–2). Previous work has demonstrated that Goldshield products have bactericidal properties [ [23] , [24] , [25] ]. For example, Goldshield 5 used as a coating on masks had been shown to be an effective antibacterial agent against both gram-positive and gram-negative bacteria [ 26 ].…”