Evaluation of Bacteriocin Activities among Enterococcal Poultry Isolates from East Azarbaijan Iran

Javaherzadeh, Vahid; Jamshidian, M; Zahraei, Mohammadtaghi; Youseftabar, Arash; Milani, Morteza; Hassan, Maryam; Lotfipour, Farzaneh

doi:10.15171/ps.2015.20

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Novel Microbial Methods In Quality Control1

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Cited by 2 publications

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“…Furthermore multiplex PCR method was applied by our group in several studies to rapidly identify the entrococci in clinical, dairy and poultry samples. [41][42][43][44] In IMS systems, paramagnetic beads are coated with a variety of molecules (like antibodies, antigens and DNA) to capture target cells (like E. coli O157, Listeria). Then, the beads are plated on agar.…”

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An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

et al. 2016

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Section: Novel Microbial Methods In Quality Controlmentioning

confidence: 99%

“…Multiplex PCR (as diagnostic method) Clinical, dairy and poultry samples Rapidly identify the entrococci in clinical, dairy and poultry samples [41][42][43][44] …”

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An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

et al. 2016

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Screening of the Enterocin-Encoding Genes and Their Genetic Determinism in the Bacteriocinogenic Enterococcus faecium GHB21

Merzoug

Mosbahi

Walker

et al. 2018

Probiotics & Antimicro. Prot.

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Enterococci are well-known for their ability to produce a variety of antimicrobial peptides called enterocins. Most of these enterocins withstand extreme conditions and are very effective against a broad spectrum of undesirable bacteria including some Gram-negative bacteria. The same enterococci strain can produce multiple enterocins simultaneously. The genetic determinants of these bacteriocins can either be located on plasmids or on bacterial chromosome. Digestion of Enterococcus faecium GHB21 plasmids with various restriction endonucleases suggests the presence of two plasmids named pGHB-21.1 and pGHB-21.2 whose respective sizes are ~ 10.0 kb and ~ 3.3 kb. The screening of enterocin-encoding genes among E. faecium GHB21 genome by PCR followed by amplicon sequencing indicated the presence of three different enterocin structural genes similar to entA, entB, and entP genes previously detected in other E. faecium strains. These enterocin genes were, subsequently, localized on the bacterial chromosome based on PCR-targeted screening using total DNA and plasmids of E. faecium GHB21 as separate templates.

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Evaluation of Bacteriocin Activities among Enterococcal Poultry Isolates from East Azarbaijan Iran

Cited by 2 publications

References 43 publications

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

Screening of the Enterocin-Encoding Genes and Their Genetic Determinism in the Bacteriocinogenic Enterococcus faecium GHB21

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