Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

doi:10.47278/journal.ijvs/2021.064

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Another study showed that GTE contains catechins, epi catechins, gallocatechin, epigallocatechin, gallocatechin gallate, and Epigalocathecin gallate (EGCG). EGCG is the most promising bioactive compound due to its strong antioxidant activity [ 19 ], in turn improving sperm motility, and viability, and increase fertility [ 20 , 21 ]. EGCG is a kind of catechin that makes up 13% of the total polyphenols in GTE and can only be found naturally in GTE, while other types of catechins can only be found in fruits and vegetables [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

et al. 2023

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Background:The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, using an extender material in addition to semen increasing spermatozoa's survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation.Methods: Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The divided samples into four treatments: T0 (no GTE added to the extender), T1 (0.05 mg GTE plus 100 mL extender), T2 (0.10 mg GTE plus 100 mL extender), and T3 (0.15 mg GTE plus 100 mL extender). The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and postthawing.Results: There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion:Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.

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Section: Discussionmentioning

confidence: 99%

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

et al. 2023

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“…To ensure the validity and reliability of inbreeding (IB) results obtained with sexing through the 5-10% BSA column, molecular verification needs to be conducted using the duplex PCR method. The applications of IB combined with spermatozoa sexing in several regions of Indonesia have achieved a sex match rate of 76-89% in field calves (Gunawan et al 2015;Sawitri et al 2021). Therefore, this study aims to molecularly verify sexing results of spermatozoa carrying X and Y chromosomes using the PCR method.…”

Section: Introductionmentioning

confidence: 99%

Molecular Verification of Spermatozoa Sexing Method in Pesisir Cattle Bull Using Bovine Serum Albumin Column

2023

Int J Vet Sci

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An accurate sexing process is crucial for preserving the purity of Pesisir cattle bulls and enhancing their productivity to meet the increasing beef demand in Indonesia. Therefore, this study aims to verify the separation of spermatozoa carrying X and Y chromosomes molecularly, using the Bovine Serum Albumin (BSA) column. The spin-column method was employed to isolate the separated X and Y spermatozoa. Amplification through PCR was performed using two sets of primers targeting the Sex-determining Region Y (SRY) gene, located on the Y chromosome, and the Ausal Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene on the X chromosome. The assessment of spermatozoa quality in coastal cattle bulls showed an average fresh semen volume, and sperm concentration to be 3±0.7mL, and 1.58±1.05x10 6 /mL, respectively. While the other parameters of assessment of spermatozoa quality including average motility, spermatozoa survival, sperm abnormalities, and mean progressive motility (MPU) were to be 80±10, 85.83±7.59, 6.36±3.50, and 79.81±5.97%, respectively. The quality evaluation after sexing included the average motility of spermatozoa with X at 56.66±11.54 and Y at 66.66±11.54%, as well as their average intact plasma membrane (IPM) being 55.58±1.01 and 59.35±7.78%, respectively. The quality of spermatozoa decreased by 20-30% after sexing. The results of spermatozoa separation with the 5% BSA column confirmed one GAPDH band (415bp), indicating the content to be X. In contrast, the 10% BSA column and the non-sexed spermatozoa exhibited two bands, SRY (318bp) and GAPDH (415bp), indicating a higher proportion of Y in the 10% BSA column. These demonstrated molecular verification of sexed spermatozoa using the 5 and 10% BSA column, enabling the separation of those carrying X and Y chromosomes in Pesisir bulls through the duplex PCR method.

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The detection of Pregnancy Associated Glycoproteins in the blood Serum as an Indicator of Pregnancy in Bali Cattle

Puja,

Sulabda,

Trinayani

et al. 2024

JAHP

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T he inability of national meat production in Indonesia has resulted in the government still importing beef from several countries. Of course, this indicates that beef production still needs to be put up to target (Sawitri et al., 2021). Efforts can be made to improve the livestock system in Indonesia, especially to achieve the target of one-year birth intervals or to shorten the calving-conception interval by detecting early pregnancy in cows after mating (Kaya et al., 2016). Early pregnancy diagnosis is necessary after mating for early removal of non-pregnant livestock. Early pregnancy diagnosis with a high level of accuracy is very important to improve the reproductive performance of cows (Abdullah et al., 2014;Lavon et al., 2022). Early pregnancy diagnosis is very important to determine Short communicationAbstract | Detection of early pregnancy in livestock is an important part of livestock reproductive management; therefore, choosing an accurate method for diagnosing early pregnancy is very important. This research aims to determine the potential utilization of pregnancy associated glycoproteins (PAG) circulating in the blood as a diagnostic tool for pregnancy in cows. A study was carried out on 37 Bali cows with previous experience giving birth. Blood samples were taken from the jugular vein on the 45th day after artificial insemination and stored at -20°C until PAG testing using the ELISA method. The sensitivity of the test for pregnancy detection was 0.33 ng/mL. As a control, samples from non-pregnant cows were used. The PAG concentration in pregnant Bali cows was 0.55 ng/mL, and in non-pregnant cows, it was 0.06 ng/mL. PAG concentration in pregnant cows was significantly different from that of cows that were not pregnant. The results of research on Bali cows showed that the accuracy of the PAG test in diagnosing pregnancy was 100%. Therefore, it was concluded that the circulating concentration of PAG is a suitable marker for determining pregnancy in Bali cattle. However, in order to better manage the reproductive system of Bali cows, further research is needed to increase the prediction value to a more acceptable starting point.

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Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

Cited by 3 publications

References 19 publications

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

Molecular Verification of Spermatozoa Sexing Method in Pesisir Cattle Bull Using Bovine Serum Albumin Column

The detection of Pregnancy Associated Glycoproteins in the blood Serum as an Indicator of Pregnancy in Bali Cattle

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