Evaluation of barley genotypes for the content of β-glucans in grain and other valuable features in Eastern Siberia

Abstract

Background.The aim of the research was to identify barley germplasm accessions with the minimum and maximum content of β-glucans in their grain in combinations with other improved useful traits.Material and methods.The material for analysis were 18 hulled and 8 naked barley accessions of various ecogeographic origin from the collection of the N.I. Vavilov AllRussian Institute of Plant Genetic Resources (VIR), which were grown under the conditions of Eastern Siberia in 2016–2018.Results.Hulled accessions yielded grains with β-glucan content from 3.18 to 4.51%; naked ones, from 3.18 to 5.21%. According to the minimum value of the studied qualitative trait (3.18–3.43%), cultivars of Siberian breeding were identified: ‘Mayak’ (k-29622, Krasnoyarsk Territory) and ‘Tarsky 3’ (k-30719, Omsk Province), plus cv. ‘AC Albright’ (k-30599, Canada); according to the maximum value (5.06–5.21%), the naked accessions Nudum 155 (k-13328, Ukraine) and ‘Nudum 95’ (k-31125, Chelyabinsk Province). The highest protein content in grain (16.63–18.36%) was found in the accessions Nudum 155, Nudum 7566 (k-29453, Kyrgyzstan) and ‘Nudum 95’. The accessions Nudum 155 and ‘Nudum 95’ were characterized by combined high contents of β-glucans and protein in their grain. Early maturity and increased productivity were shown by the cultivars ‘Tarsky 3’, ‘Kolchan’ (k-31039, Altai Territory), ‘Zolotnik’ (k-30845, Altai Territory), ‘Abalak’ (Krasnoyarsk Territory, Tyumen Province), and ‘AC Albright’ (Canada). A significant positive relationship was found between the content of β-glucans and the weight of 1000 grains in both forms of barley.Conclusion.According to β-glucan content combined with other valuable characteristics in grain, the accessions ‘Mayak’, ‘Tarsky 3’ and ‘AC Albright’ are of interest for the improvement of fodder cultivars, while ‘Nudum 95’ and Nudum 155 are promising for breeding for food.

Weight method for determination of soluble β-glucans in barley grain

“…The results were compared with the data obtained on the same barley accessions by the arbitration enzymatic method AOAC 995.16 and ICC Standard Method No. 168 for β-glucans (Megazyme) (Polonskiy et al, 2021). The developed method was tested on naked and covered barley cultivars (Table 1).…”

Higher stability of oat, barley and wheat accessions in their 1000 grain weight is not associated with a smaller grain size

“…В связи с происходящим сегодня реальным изменением климата на планете, а также для территорий с резко континентальными погодными условиями, к которым относится Восточная Сибирь, актуально наличие сортов сельскохозяйственных культур, проявляющих не только высокую продуктивность и качество урожая, но и повышенную стабильность хозяйственно полезных признаков. Для указанного региона основными являются зерновые культуры, среди которых к приоритетным относятся яровые пшеница, овес и ячмень, в зерне которых содержится значительное количество биологически активных веществ, полезных для здоровья человека (Polonskiy et al, 2019;Loskutov, Khlestkina, 2021;Polonskiy et al, 2021;Shvachko et al, 2021;Shewry et al, 2022).…”