Evaluation of basal micronucleus frequency and hexavalent chromium effects in fish erythrocytes

Lemos, Clarice Torres de; Rödel, Patrícia Milan; Terra, Nara Regina; Erdtmann, Bernardo

doi:10.1002/etc.5620200621

Cited by 64 publications

(34 citation statements)

References 28 publications

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“…The researchers also proposed the mean of 2.0 ± 3.3 x 10 -4 for micronucleus erythrocytes. This frequency baseline is similar to those presented in several fish species studied fish species (Al-Sabti & Metcalfe, 1995;Grisolia & Starling, 2001;Lemos et al, 2001;Bolognesi et al, 2006). Considering this baseline value, A. jacuhiensis from rio Uruguai basin present in both environments and seasons analyzed values higher than the one pointed as baseline for Lemos et al (2008).…”

Section: Discussionsupporting

confidence: 87%

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

et al. 2015

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Due to intense agricultural activity in the rio Uruguai (South Brazil), there is the potential for aquatic contamination by agrochemicals. In this region, there are many reservoirs to meet the water demand for rice fields, forming lentic environments. In line with this information, the aim of this study was to show a comparative analysis of some biomarkers, such as lipid peroxidation (TBARS), gluthatione S-transferase (GST), non-protein thiols (NPSH), amino acids (AA) and piscine micronucleus tests (MNE) in Astyanax jacuhiensis from lentic and lotic environments in the middle rio Uruguai region, comparing warm and cold seasons. Eight pesticides were found in water samples, with propoxur having the highest concentration found in both environments and seasons. Fish from the warm season showed higher levels of biochemical biomarkers, and fish from the cold season showed higher levels of MNE and AA. TBARS and AA presented higher levels in fish from the river, while GST, NPSH, MNE and AA presented higher levels in fish from dams. These environments have different characteristics in terms of redox potential, aeration, sedimentation, trophic structure, agrochemicals input and others, which may affect the physiological and biochemical responses of fish in against adverse situations.Devido à intensa atividade agrícola no rio Uruguai (Sul do Brasil), há potencial para contaminação aquática por agrotóxicos. Há muitos reservatórios para atender a demanda de água de campos de arroz, formando ambientes lênticos. De acordo com estas informações, o objetivo do presente estudo foi mostrar uma análise comparativa de alguns biomarcadores como a peroxidação lipídica (TBARS), glutationa S-transferase (GST), tióis não-protéicos (NPSH), aminoácidos (AA) e teste písceo de micronúcleos (MNE) em Astyanax jacuhiensis amostrados em ambientes lóticos e lênticos da região do médio rio Uruguai, comparando estações quentes e frias. Oito pesticidas foram encontrados em amostras de água, sendo propoxur a maior concentração encontrada em ambos os ambientes e estações. Peixes da estação quente apresentaram maiores níveis de biomarcadores bioquímicos e peixes da estação fria apresentaram maiores níveis de MNE e AA. TBARS e AA apresentaram maiores níveis nos peixes de rio, enquanto GST, NPSH, MNE e AA apresentaram níveis mais elevados em peixes da represa. Estes ambientes têm características diferentes, com potencial redox, aeração, sedimentação, estrutura trófica, a entrada de agroquímicos e outros que podem afetar as respostas fisiológicas e bioquímicas de peixe contra situação adversa.

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Section: Discussionsupporting

confidence: 87%

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

et al. 2015

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“…This is in agreement with some (De Lemos et al, 2001;Cavas et al, 2005) studies that have reported decrease in the number of MN in fish erythrocytes after 14-21 of exposure. This could mean that DNA repair occurred after the day 14 onwards.…”

Section: Discussionsupporting

confidence: 82%

Cytotoxic and genotoxic effects of perfluorododecanoic acid (PFDoA) in Japanese medaka

Ayanda

Yang

Zhang

et al. 2018

Knowl. Manag. Aquat. Ecosyst.

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-This study investigated the cytotoxic and genotoxic potential of perfluorododecanoic acid (PFDoA), a perfluorinated carboxylic chemical (PFC) that has broad applications and distribution in the environment in Japanese medaka, Oryzias latipes. Micronucleus (MN) test and Comet assay were used for the toxicity study. Three groups of fish were exposed to 0.1 mg/L, 0.5 mg/L and 2.5 mg/L concentration of the chemical for 28 days. Another group served as control. Sampling of the fish blood and liver were done after days 1, 4, 7, 14, 21 and 28 for analysis of different erythrocyte abnormalities and damage to DNA using the MN test and Comet assay respectively. Results showed that there was a significant time and concentration dependent increase (p < 0.05) in percent tail length of DNA and frequency of erythrocyte abnormalities. Nuclear abnormalities observed include micronucleus, fragmented apoptotic cells, lobed nuclei, and bean-shaped cells. Increase in induction of erythrocyte abnormalities and percent tail length of DNA peaked at days 14 and 7, respectively, after which there was a gradual decline. The results indicate that sub-chronic exposure of PFDoA to Japanese medaka caused DNA damage with a simultaneous induction of different erythrocyte abnormalities.

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“…Induction of micronuclei was reported previously for fish exposed to Cd [Ayllon and Garcia Vazquez, 2000;Sanchez-Galan et al, 2001;Zhu et al, 2004;Arkhipchuk and Garanko, 2005;Cavas et al, 2005], Cu [Zhu et al, 2004;Arkhipchuk and Garanko, 2005;Cavas et al, 2005], Cr [Al-Sabti et al, 1994;de Lemos et al, 2001;Zhu et al, 2004], and Pb Ferraro et al, 2004]. Therefore, it is plausible that elevated concentrations of heavy metals could have contributed to the elevated MN and NA frequencies produced by the downstream Berdan River water samples.…”

Section: Discussionmentioning

confidence: 75%

Evaluation of river water genotoxicity using the piscine micronucleus test

Ergene

Çavaş

Çelık

et al. 2007

Environ and Mol Mutagen

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The Berdan River, which empties into the Mediterranean Sea on the east coast of Turkey, receives discharges of industrial and municipal waste. In the present study, the in vivo piscine micronucleus (MN) test was used to evaluate the genotoxicity of water samples collected from different locations along the Berdan River. Nile tilapia (Oreochromis niloticus) were exposed in the laboratory for 2, 4, and 6 days, and micronuclei were evaluated in peripheral blood erythrocytes, gill cells, and caudal fin epithelial cells. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear abnormalities (NAs), such as binucleated cells and blebbed, notched, and lobed nuclei, were assessed in the erythrocytes, and chemical analyses were carried out to determine the amount of heavy metals in the water samples. MN and NA frequencies were significantly elevated (up to 2- to 3-fold) in fish exposed to river water samples taken downstream of potential discharges, and the elevated responses in gill and fin cells were related to the concentration of heavy metals in the water. MN frequencies (expressed as micronucleated cells/1,000 cells), in both treated and untreated fish, were greatest in gill cells (range: 0.80-3.70), and generally lower in erythrocytes (range: 0.50-2.80), and fin cells (range: 0.45-1.70). The results of this study indicate that the Berdan River is contaminated with genotoxic pollutants and that the genotoxicity is related to the discharge of wastes into the river water.

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Evaluation of basal micronucleus frequency and hexavalent chromium effects in fish erythrocytes

Cited by 64 publications

References 28 publications

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil

Cytotoxic and genotoxic effects of perfluorododecanoic acid (PFDoA) in Japanese medaka

Evaluation of river water genotoxicity using the piscine micronucleus test

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