Evaluation of Bauhinia variegata L. bark fractions for in vitro antioxidant potential and protective effect against H2O2-induced oxidative damage to pBR322 DNA

Sharma, Neha

doi:10.5897/ajpp11.457

Cited by 16 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results of the presented work supported the previously reported antimutagenic activity of Kigelia africana leaves flavonoids assessed using cyclophosphamide induced micronuclei formation and chromosomal aberrations in mouse bone marrow [36]. It has previously been demonstrated that methanol extracts of Bauhinia variegata bark shows protective effect against H 2 O 2 induced pBR322 DNA damages [37] and cyclophosphamide induced micronuclei formation in Swiss bone marrow cells [16].…”

Section: Discussionsupporting

confidence: 88%

Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay

Abdul-Hafeez

Karamova

Ilinskaya

2018

Ecological genetics

View full text Add to dashboard Cite

Stem bark aqueous extracts of eight woody plants Brachychiton populneus, Ceiba pentandra, Bombax malabaricum, Chorisia speciosa, Albizia lebbeck, Bauhinia variegata, Kigelia africana and Pinus halepensis were tested for their mutagenic and antimutagenic potential in the Ames test with Salmonella typhimurium strains TA98 and TA100. The aqueous extracts were neither toxic nor mutagenic in S. typhimurium tester strains. All of the tested extracts showed detectable antimutagenic effect towards the direct acting mutagens 2-nitrofluorene (2-NF) in TA98 as well as sodium azide in TA100. The extract from Kigelia africana was the most effective in reducing the mutagenicity caused by the direct mutagen 2-NF in the TA98 with 85.42% inhibition rate. A. lebbeck stem bark extract demonstrated the highest antimutagenic activity reducing the base substitution mutations rate for strain TA100 by 94.66% in pre-incubation assay. The results obtained showed that the stem bark aqueous extracts tested can protect cells against induced gene mutations.

show abstract

Section: Discussionsupporting

confidence: 88%

Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay

Abdul-Hafeez

Karamova

Ilinskaya

2018

Ecological genetics

View full text Add to dashboard Cite

show abstract

“…Further, an increase in superoxide dismutase, catalase and total proteins was also observed [66]. A phytochemical investigation of the polar fractions of methanol extract of B. variegata bark revealed that the fractions were rich in phenolic and flavonoid content and a statistically significant correlation was observed between phenolic/flavonoid content and the antioxidant activity of the fractions [67]. The essential oil of B. variegata flowers has been reported to contain a substantial amount of nerolidol, α-bisabolol and β-bisabolene which have significant medicinal properties [68].…”

Section: Discussionmentioning

confidence: 99%

Isolation of Phytochemicals from Bauhinia variegata L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential

Sharma

Bhatia

et al. 2019

Antioxidants

Self Cite

View full text Add to dashboard Cite

Plants have been the basis of traditional medicine since the dawn of civilizations. Different plant parts possess various phytochemicals, playing important roles in preventing and curing diseases. Scientists, through extensive experimental studies, are playing an important part in establishing the use of phytochemicals in medicine. However, there are still a large number of medicinal plants which need to be studied for their phytochemical profile. In this study, the objective was to isolate phytochemicals from bark of Bauhinia variegata L. and to study them for their antioxidant and cytotoxic activities. The bark was extracted with methanol, followed by column chromatography and thus isolating kaempferol, stigmasterol, protocatechuic acid-methyl ester (PCA-ME) and protocatechuic acid (PCA). 2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 2, 2’-diphenyl-1-picrylhydrazyl radical (DPPH) radical scavenging assays were utilized for assessment of antioxidant activity, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye reduction assay was used to determine cytotoxic activity against C-6 glioma rat brain, MCF-7 breast cancer, and HCT-15 colon cancer cell lines. The compounds were found to have significant antioxidant and cytotoxic activity. Since there is a considerable increase in characterizing novel chemical compounds from plant parts, the present study might be helpful for chemotaxonomic determinations, for understanding of medicinal properties as well as for the quality assessment of herbal supplements containing B. variegata bark, thus establishing its use in traditional medicine.

show abstract

“…Samples of No. 12, 18, and 21 used in this study have been reported to have antioxidant contents, including phenolic, flavonoid, and tannic [20,21]. Thus, the samples of No.…”

Section: Discussionmentioning

confidence: 99%

In vitrobiological evaluation of 100 selected methanol extracts from the traditional medicinal plants of Asia

Wang

2014

Nutr Res Pract

View full text Add to dashboard Cite

BACKGROUND/OBJECTIVES:In Asia, various medicinal plants have been used as the primary sources in the health care regimen for thousands of years. In recent decades, various studies have investigated the biological activity and potential medicinal value of the medicinal plants. In this study, 100 methanol extracts from 98 plant species were evaluated for their biological activities. MATERIALS/METHODS:The research properties, including 1,1-diphenyl-2-pic-rylhydrazyl (DPPH) radical scavenging activity, α -glucosidase and α-tyrosinase inhibitory effects, anti-inflammatory activity, and anticancer activity were evaluated for the selected extracts. RESULTS: Fifteen of the extracts scavenged more than 90% of the DPPH radical. Among the extracts, approximately 20 extracts showed a strong inhibitory effect on α-glucosidase, while most had no effect on α-tyrosinase. In addition, 52% of the extracts showed low toxicity to normal cells, and parts of the extracts exhibited high anti-inflammatory and anticancer activities on the murine macrophage cell (RAW 264.7) and human colon cancer cell (HT-29) lines, respectively. CONCLUSIONS: Our findings may contribute to further nutrition and pharmacological studies. Detailed investigations of the outstanding samples are currently underway.

show abstract

Evaluation of Bauhinia variegata L. bark fractions for in vitro antioxidant potential and protective effect against H2O2-induced oxidative damage to pBR322 DNA

Cited by 16 publications

References 31 publications

Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay

Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay

Isolation of Phytochemicals from Bauhinia variegata L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential

In vitrobiological evaluation of 100 selected methanol extracts from the traditional medicinal plants of Asia

Contact Info

Product

Resources

About