Evaluation of BD Vacutainer SST™ II plus tubes for special proteins testing

Li, Zhiyan; Yan, Cunling; Yan, Rong; Zheng, Xiaoyan; Feng, Zhenru

doi:10.1002/jcla.20458

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Samples were obtained from a single cheek-puncture for each individual mouse. Whole blood samples were collected using BD Vacutainer SST Blood Collection Tubes (BD Worldwide; Product Number: 367381) for serum separation as described previously ( 20 ). Specimens were centrifuged at 1,100 × g for 20 min after clotting for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis

et al. 2017

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There is an urgent need in multiple sclerosis (MS) patients to develop biomarkers and laboratory tests to improve early diagnosis, predict clinical relapses, and optimize treatment responses. In healthy individuals, the transport of proteins across the blood–brain barrier (BBB) is tightly regulated, whereas, in MS, central nervous system (CNS) inflammation results in damage to neuronal tissues, disruption of BBB integrity, and potential release of neuroinflammatory disease-induced CNS proteins (NDICPs) into CSF and serum. Therefore, changes in serum NDICP abundance could serve as biomarkers of MS. Here, we sought to determine if changes in serum NDICPs are detectable prior to clinical onset of experimental autoimmune encephalomyelitis (EAE) and, therefore, enable prediction of disease onset. Importantly, we show in longitudinal serum specimens from individual mice with EAE that pre-onset expression waves of synapsin-2, glutamine synthetase, enolase-2, and synaptotagmin-1 enable the prediction of clinical disease with high sensitivity and specificity. Moreover, we observed differences in serum NDICPs between active and passive immunization in EAE, suggesting hitherto not appreciated differences for disease induction mechanisms. Our studies provide the first evidence for enabling the prediction of clinical disease using serum NDICPs. The results provide proof-of-concept for the development of high-confidence serum NDICP expression waves and protein biomarker candidates for MS.

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Section: Methodsmentioning

confidence: 99%

Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis

et al. 2017

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“…Some of these limitations include inappropriate performance in prolonged storage of the blood samples and difficulties for suitable separation of the blood serum from red blood cells. Moreover, long contact between serum and blood cells may change the serum color from yellow to red [6]. In order to overcome these limitations and issues, the blood serum separator tubes (SST) was introduced to laboratories in 1976 by Becton Dickinson [7].…”

Section: Introductionmentioning

confidence: 99%

A New Formulation for Polymeric Separator Gels for Potential use in Blood Serum Separator Tubes

Babakhani

Movahed

Ghazy

2018

Progress in Rubber, Plastics and Recycling Technology

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Blood serum separator tubes (SST) are used for collecting blood samples for performing clinical chemistry assays. Some SST's have separator gels inside the tube which enable them better separation of the blood serum from packed cells during centrifugation. The cost, weak performance and interaction with blood ingredients are the most concerns of the available commercial gels. A commercial and cheap silicone oil as a polymeric base of the gel was chosen and formulated without and with several fillers. Subsequently, the compounds were crosslinked through a free radical crosslinking mechanism using dicumyl peroxide (DCP). The crosslinking took place in both, an oven and as well as under microwave irradiation in normal and under pressure conditions. The FTIR spectrometer analysis showed that both chain ends of the used silicone oil were terminated with a vinyl group. It also revealed that blood serum separator gel can be produced from selected silicone oil type. Among of different curing apparatus, curing in an oven was preferred due to less curing time and electrical energy consumption. The curing in normal pressure showed better results when compared with curing under pressure. Increasing the filler and DCP with various amounts had positive effect on gel densities. Silica was the most efficient filler among of the studied fillers. The cured compound filled with 10 and 8 phr silica and DCP, respectively, was chosen as appropriate gel for SST due to suitable density and thixotropy. The selected gel was cured in oven under normal pressure for 30 minutes at 160°C.

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“…20 LI, et al, 2011 (22) Evaluation of BD Vacutainer SST TM II Plus Tubes for Special Proteins Testing Dependendo das condições de coleta, manuseio e processamento da amostra, alterações clinicamente significativas nas concentrações de analito podem ocorrer nos tubos separadores de soro.…”

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“…Ocasionalmente, as partículas do ativador de coágulo podem não se aglomerar completamente com ele e permanecerem na camada de soro, causando interferências ao revestir os dispositivos de pipetagem, principalmente durante o armazenamento do tubo de coleta em temperaturas inadequadas. (19,21,22) Essa é uma das muitas causas responsáveis por aproximadamente 70% do total de erros em testes laboratoriais ainda ocorrerem na fase pré-analítica, sendo uma fonte potencial de distorção dos resultados. Vale salientar que o tipo de tubo usado para amostragem de soro, em particular tubos com gel de barreira (tubos de gel), pode fazer com que os analitos sejam adsorvidos durante o armazenamento.…”

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Interferências do gel separador em análises bioquímicas e possíveis soluções: uma revisão bibliográfica

Oliveira¹,

Bandeira²,

Batista³

et al. 2021

RBAC

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Este trabalho é uma revisão bibliográfica sobre as interferências dos tubos de coleta com gel separador em análises bioquímicas, comparando estes resultados com os obtidos em tubos com separador mecânico (Barricor). Para tanto, buscamos artigos nas bases de dados PubMed, Google acadêmico, Scielo e LILACS. Após a aplicação dos critérios de inclusão e exclusão, foram selecionados 21 artigos para a escrita da revisão. Estes trabalhos mostraram que o gel pode causar instabilidade nas dosagens de íons e de diversas substâncias orgânicas. Além disso, em alguns casos a análise da amostra fica impossibilitada devido ao fenômeno de formação do gel flutuante. No entanto, através do uso do tubo com separador mecânico foi possível minimizar essas interferências e instabilidades. Outra vantagem está relacionada ao tempo reduzido de centrifugação da amostra com o tubo de separador mecânico (3 min. vs. 10 min.). Este tipo de separação também permite uma centrifugação mais eficaz da amostra, com menor contaminação do plasma com células e plaquetas, quando comparada à centrifugação do soro obtido nos tubos tradicionais com gel separador. Por estas razões, fica evidente que as amostras de plasma obtidas em tubos de separação mecânica demonstram ser de melhor qualidade para as análises bioquímicas.

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Evaluation of BD Vacutainer SST™ II plus tubes for special proteins testing

Cited by 6 publications

References 12 publications

Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis

Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis

A New Formulation for Polymeric Separator Gels for Potential use in Blood Serum Separator Tubes

Interferências do gel separador em análises bioquímicas e possíveis soluções: uma revisão bibliográfica

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