Evaluation of Beauveria brongniartii and its Metabolite Oosporein Regarding Phytotoxicity on Seed Potatoes

Abendstein, Daniela; Pernfuß, B.; Strasser, Hermann

doi:10.1080/09583150020017235

Cited by 11 publications

(5 citation statements)

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“…Oosporein is a red dibenzoquinone pigment initially reported in Oospora colorans (7) and subsequently found in a number of soil and endophytic fungi, as well as in the entomopathogenic Beauveria genus (8)(9)(10)(11). The physiochemical properties of oosporein have been explored, and its chemical synthesis has been reported (12)(13)(14).…”

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confidence: 99%

Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death

Fan

Liu

Keyhani

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

124

119

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The regulatory network and biological functions of the fungal secondary metabolite oosporein have remained obscure. Beauveria bassiana has evolved the ability to parasitize insects and outcompete microbial challengers for assimilation of host nutrients. A novel zinc finger transcription factor, BbSmr1 (B. bassiana secondary metabolite regulator 1), was identified in a screen for oosporein overproduction. Deletion of Bbsmr1 resulted in up-regulation of the oosporein biosynthetic gene cluster (OpS genes) and constitutive oosporein production. Oosporein production was abolished in double mutants of Bbsmr1 and a second transcription factor, OpS3, within the oosporein gene cluster (ΔBbsmr1ΔOpS3), indicating that BbSmr1 acts as a negative regulator of OpS3 expression. Real-time quantitative PCR and a GFP promoter fusion construct of OpS1, the oosporein polyketide synthase, indicated that OpS1 is expressed mainly in insect cadavers at 24-48 h after death. Bacterial colony analysis in B. bassiana-infected insect hosts revealed increasing counts until host death, with a dramatic decrease (∼90%) after death that correlated with oosporein production. In vitro studies verified the inhibitory activity of oosporein against bacteria derived from insect cadavers. These results suggest that oosporein acts as an antimicrobial compound to limit microbial competition on B. bassiana-killed hosts, allowing the fungus to maximally use host nutrients to grow and sporulate on infected cadavers.Beauveria bassiana | oosporein | biological role | transcription factor | fungal-bacterial competition

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mentioning

confidence: 99%

Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death

Fan

Liu

Keyhani

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

124

119

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“…Petri dish cultures of B. brongniartii (BIPESCO 2) were grown on Sabouraud-2-Glucose agar medium at 25 °C for 9 days as described in the literature 31. Petri dish cultures of M. brunneum (BIPESCO 5) were cultivated on Sabouraud-4-Glucose agar medium at 25 °C for 12 days.…”

Section: Methodsmentioning

confidence: 99%

A convenient workflow to spot photosensitizers revealed photo-activity in basidiomycetes

et al. 2019

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“…Oosporein is a quinone-type antifungal and antibacterial metabolite produced by Beauveria brongniartii (Abendstein et al, 2000) that exhibits a significant growth-inhibitory effect on Phytophthora infestans in comparison with other phytopathogenic fungi (Nagaoka et al, 2004).…”

Section: Antibiotics (Antifungal and Antibacterial Metabolites)mentioning

confidence: 99%

Scientific support, literature review and data collection and analysis for risk assessment on microbial organisms used as active substance in plant protection products –Lot 1 Environmental Risk characterisation

Mudgal

Toni

Tostivint

et al. 2013

EFSA Supporting Publications

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Evaluation of Beauveria brongniartii and its Metabolite Oosporein Regarding Phytotoxicity on Seed Potatoes

Cited by 11 publications

References 1 publication

Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death

Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death

A convenient workflow to spot photosensitizers revealed photo-activity in basidiomycetes

Scientific support, literature review and data collection and analysis for risk assessment on microbial organisms used as active substance in plant protection products –Lot 1 Environmental Risk characterisation

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