Evaluation of bifidobacteria for the production of antimicrobial compounds and assessment of performance in cottage cheese at refrigeration temperature

O’Riordan,; Fitzgerald,

doi:10.1046/j.1365-2672.1998.00474.x

Cited by 68 publications

(37 citation statements)

References 26 publications

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“…Bi®dobacteria are currently attracting considerable scienti®c interest due to increasing recognition of their health promoting potential [5]. These organisms have variously been implicated in competition with pathogenic and putrefactive bacteria for nutrients and attachment sites in the gut [6], immune stimulation [7], production of organic acids that acidify the large intestine and the formation of substances that inhibit the growth of human pathogens [8,9].…”

Section: Introductionmentioning

confidence: 99%

Effect of short-chain carbohydrates on human intestinal bifidobacteria and Escherichia coli in vitro

Sharp¹,

Fishbain

Macfarlane

2001

Journal of Medical Microbiology

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Plate counts and small subunit (SSU) rRNA abundance were used to study the effects of fructo-oligosaccharides (FOS), fructose, or galacto-oligosaccharides (GOS) on bi®dobac-terial populations in human faecal microbiotas. The bacteria were grown in pHcontrolled anaerobic fermentation vessels. Untreated cultures and fructose-amended fermenters were used as controls. Bi®dobacterium longum, B. adolescentis and B. angulatum comprised the dominant bi®dobacterial populations throughout the experiment. No major differences were found in the four treatments, in terms of viable counts of the organisms or of total populations of bi®dobacteria at any time point. However, large differences were observed with respect to the abundance of bi®dobacterial SSU rRNA between the treatments. Greatest bi®dobacterial SSU rRNA abundance was seen in FOS cultures, with the lowest in the untreated control fermentation. GOS and fructose also increased bi®dobacterial SSU rRNA. Cultures supplemented with FOS and GOS were also associated with lower colony counts and SSU rRNA abundance for Escherichia coli, compared with fructose-supplemented and control fermenters. At the 24-h time point, the untreated control contained 19.8 ìg of enterobacterial SSU rRNA/ml of culture¯uid, compared with 11.4 ìg/ml for the fructose fermentation, and 2.6 and 0.5 ìg/ml for the FOS and GOS culture vessels, respectively.

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Section: Introductionmentioning

confidence: 99%

Effect of short-chain carbohydrates on human intestinal bifidobacteria and Escherichia coli in vitro

Sharp¹,

Fishbain

Macfarlane

2001

Journal of Medical Microbiology

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“…In order to efficiently exploit this information, we used a proteomic approach to analyze the regulation of bifidobacterial proteins during growth in the presence of bile salts by separation through two-dimensional (2D) electrophoresis and subsequent identification by MALDI-TOF mass spectrometry. B. longum NCIMB 8809, a human isolate with the capacity to produce an antimicrobial substance (40), was chosen as a model microorganism for this study. To the best of our knowledge, this is the first report dealing with the Bifidobacterium response to bile at a molecular level and constitutes the first proteomic analysis of this genus under stress conditions.…”

mentioning

confidence: 99%

Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809

Sánchez

Champomier‐Vergès²,

Anglade³

et al. 2005

J Bacteriol

179

171

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Adaptation to and tolerance of bile stress are among the main limiting factors to ensure survival of bifidobacteria in the intestinal environment of humans. The effect of bile salts on protein expression patterns of Bifidobacterium longum was examined. Protein pattern comparison of strains grown with or without bile extract allowed us to identify 34 different proteins whose expression was regulated. The majority of these proteins were induced after both a minor (0.6 g liter ؊1 ) and a major (1.2 g liter ؊1 ) exposure to bile. These include general stress response chaperones, proteins involved in transcription and translation and in the metabolism of amino acids and nucleotides, and several enzymes of glycolysis and pyruvate catabolism. Remarkably, xylulose 5-phosphate/fructose 6-phosphate phosphoketolase, the key enzyme of the so-called bifidobacterial shunt, was found to be upregulated, and the activity on fructose 6-phosphate was significantly higher for protein extracts of cells grown in the presence of bile. Changes in the levels of metabolic end products (acetate and lactate) were also detected. These results suggest that bile salts, to which bifidobacteria are naturally exposed, induce a complex physiological response rather than a single event in which proteins from many different functional categories take part. This study has extended our understanding of the molecular mechanism underlying the capacity of intestinal bifidobacteria to tolerate bile.

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“…PCA showed slightly lower antimicrobial activity than lactic acid bacteria. O'Riordan and Fitzgerald [24] examined twelve strains of Bifidobacteria which exhibited a broad spectrum of antagonistic activity against both Gram-positive and Gram-negative indicators, especially Pseudomonas species, using deferred antagonism spot plate assays. Inhibitory action was shown to be unrelated to hydrogen peroxide production and not solely dependent on acidity.…”

Section: P109mentioning

confidence: 99%

Bio-control of Pseudomonas fluorescens in Domiati Cheese

Hussein¹

2015

J Adv Dairy Res

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Concerning the antimicrobial activities of some probiotics bacteria Lactobacillus acidophilus P109, Lactobacillus plantarum P164, E. durans P174 and B. longum CHRS using an agar well-diffusion as In Vitro assay against selected isolates of Pseudomonas fluorescens, Bacillus cereus, Enterococcus fecalis and Staphylococcus aureus, indicated that B. longum CHRS appeared to have antimicrobial activity against these isolates. B. longum CHRS was injected with and without Ps. fluorescens in Domiati cheese during manufacturing as In Vivo experiment, results revealed that this strain of probiotic bacteria was reduced the count of Ps. fluorescens, while the chemical composition showed reduce production of soluble nitrogen, which has relation with the decomposition of protein as well as led to reduced volatile fatty acids, which refers to the decomposition of fat as a result of antimicrobial activity of B. longum against Ps. fluorescens.

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Evaluation of bifidobacteria for the production of antimicrobial compounds and assessment of performance in cottage cheese at refrigeration temperature

Cited by 68 publications

References 26 publications

Effect of short-chain carbohydrates on human intestinal bifidobacteria and Escherichia coli in vitro

Effect of short-chain carbohydrates on human intestinal bifidobacteria and Escherichia coli in vitro

Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809

Bio-control of Pseudomonas fluorescens in Domiati Cheese

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