Evaluation of bilayer disks as plant cell membrane models in partition studies

Boija, Elisabet; Lundquist, Anna; Edwards, Katarina; Johansson, Gunnar

doi:10.1016/j.ab.2007.02.012

Cited by 36 publications

(26 citation statements)

References 30 publications

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“…In previous studies we have shown that the composition of the discs can be varied and e.g. tailored to reflect that of a biological membrane [14,15]. Further, the size of the discs can be altered by changing the PEG-lipid content [13].…”

Section: Discussionmentioning

confidence: 99%

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Influence of preparation path on the formation of discs and threadlike micelles in DSPE-PEG2000/lipid systems

Sandström

Johansson

Edwards

2008

Biophysical Chemistry

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In a recent study we showed that the surfactant 1,2-distearoyl-sn-glycero-3-phosphatidylethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG 2000 ) induce mixed micelles of either threadlike or discoidal shape when mixed with different types of lipids .In certain lipid systems the discoidal micelles adapt sizes large enough to be characterized as bilayer discs. The discs hold great potential for use in various biotechnical applications and may e.g. be used as model membranes in drug/membrane partition studies . Depending on the application, discs with certain characteristics, such as a particular size or size homogeneity, may be required. These factors can in our experience be influenced by the preparation method. In this study we systematically investigated three different PEG-lipid/lipid mixtures prepared by four commonly used preparation techniques. The techniques used were simple hydration, freezethawing, sonication and detergent depletion, and the aggregate size and structure was analyzed by cryo transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS). Our results show that the type and size of the micellar structure found, as well as the structure homogeneity of the preparation, can be modified by the choice of preparation path.

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Section: Discussionmentioning

confidence: 99%

“…The discs have e.g. been utilized as a model membranes in drug/membrane partitioning studies [13][14][15]. Results of these studies suggest that the discs, due to their stable and open structure, in many cases are superior to liposomes as model membranes.…”

Section: Introductionmentioning

confidence: 99%

Influence of preparation path on the formation of discs and threadlike micelles in DSPE-PEG2000/lipid systems

Sandström

Johansson

Edwards

2008

Biophysical Chemistry

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“…Studies of model membranes using liposomes or lipid bilayer discs demonstrate partitioning of model phenolic substrates into the membrane (Boija and Johansson, 2006;Boija et al, 2007). It is not clear how the desorption of these compounds from that hydrophobic environment into the apoplast would occur in this model.…”

Section: Discussionmentioning

confidence: 99%

Tracking Monolignols during Wood Development in Lodgepole Pine

Kaneda

Rensing²,

Wong³

et al. 2008

Plant Physiology

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Secondary xylem (wood) formation in gymnosperms requires that the tracheid protoplasts first build an elaborate secondary cell wall from an array of polysaccharides and then reinforce it with lignin, an amorphous, three-dimensional product of the random radical coupling of monolignols. The objective of this study was to track the spatial distribution of monolignols during development as they move from symplasm to apoplasm. This was done by feeding [ 3 H]phenylalanine ([ 3 H]Phe) to dissected cambium/ developing wood from lodgepole pine (Pinus contorta var latifolia) seedlings, allowing uptake and metabolism, then rapidly freezing the cells and performing autoradiography to detect the locations of the monolignols responsible for lignification. Parallel experiments showed that radioactivity was incorporated into polymeric lignin and a methanol-soluble pool that was characterized by high-performance liquid chromatography. [ 3 H]Phe was incorporated into expected lignin precursors, such as coniferyl alcohol and p-coumaryl alcohol, as well as pinoresinol. Coniferin, the glucoside of coniferyl alcohol, was detected by high-performance liquid chromatography but was not radioactively labeled. With light microscopy, radiolabeled phenylpropanoids were detected in the rays as well as the tracheids, with the two cell types showing differential sensitivity to inhibitors of protein translation and phenylpropanoid metabolism. Secondary cell walls of developing tracheids were heavily labeled when incubated with [ 3 H]Phe. Inside the cell, cytoplasm was most strongly labeled followed by Golgi and low-vacuole label. Inhibitor studies suggest that the Golgi signal could be attributed to protein, rather than phenylpropanoid, origins. These data, produced with the best microscopy tools that are available today, support a model in which unknown membrane transporters, rather than Golgi vesicles, export monolignols.

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“…The size of the disks can be varied by alteration of the PEG-lipid content (17,22). Furthermore, the lipid composition can be adjusted to closely mimic that of biological cell membranes (16,18). Similar to the case with liposomes, membrane spanning, as well as peripheral, membrane proteins can be incorporated into the lipodisks (16,23).…”

Section: Introductionmentioning

confidence: 99%

“…PEG-stabilized bilayer disks, henceforth referred to as lipodisks, have emerged as an interesting alterative to liposomes for the use as model membranes in interaction studies (16)(17)(18)(19)(20). The lipodisks are flat circular lipid aggregates consisting of a lipid bilayer surrounded by a highly curved rim (see schematic picture in Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Immobilized lipodisks as model membranes in high-throughput HPLC-MS analysis

et al. 2013

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This is an accepted version of a paper published in Analytical and Bioanalytical Chemistry. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination.Citation for the published paper: Meiby, E., Morin Zetterberg, M., Ohlson, S., Agmo Hernańdez, V., Edwards, K. (2013 AbstractLipodisks, also referred to as polyethylene glycol (PEG)-stabilized bilayer disks, have previously been demonstrated to hold great potential as model membranes in drug partition studies. In this study, an HPLC-MS system with stably immobilized lipodisks is presented. Functionalized lipodisks were immobilized on two different HPLC support materials either covalently by reductive amination or by streptavidin-biotin binding. An analytical HPLC column with immobilized lipodisks was evaluated by analysis of mixtures containing15 different drug compounds. The efficiency, reproducibility and stability of the system were found to be excellent. In situ incorporation of cyclooxygenase-1 (COX-1) in immobilized lipodisks on a column was also achieved. Specific binding of COX-1 to the immobilized lipodisks was validated by interaction studies with QCM-D. These results taken together open up for the potential to study ligand interactions with membrane proteins by weak affinity chromatography (WAC).

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Evaluation of bilayer disks as plant cell membrane models in partition studies

Cited by 36 publications

References 30 publications

Influence of preparation path on the formation of discs and threadlike micelles in DSPE-PEG2000/lipid systems

Influence of preparation path on the formation of discs and threadlike micelles in DSPE-PEG2000/lipid systems

Tracking Monolignols during Wood Development in Lodgepole Pine

Immobilized lipodisks as model membranes in high-throughput HPLC-MS analysis

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