Evaluation of biochemical responses in Palearctic and Lake Baikal endemic amphipod species exposed to CdCl2

Timofeyev, Maxim A.; Shatilina, Z. M.; Bedulina, Daria; Протопопова, М. В.; Павличенко, В. В.; Grabelnych, O.I.; Колесниченко, А. В.

doi:10.1016/j.ecoenv.2007.06.014

Cited by 24 publications

(11 citation statements)

References 40 publications

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“…A decrease in peroxidase activity at intermediate temperatures (±6 °C around the optimum) may reflect either a decrease in ROS production, or serve as an energy-saving mechanism that redirects resources to other protective mechanisms (e.g., chaperones) to ensure optimal cellular protection at high temperatures. A similar decrease in activities of antioxidant enzymes has been previously shown in response to a variety of environmental stressors in amphipods (Timofeyev et al, 2008; Timofeyev et al, 2009). No changes in activity of catalase and glutathione S-transferase were found in the freshwater or saltwater populations of amphipods.…”

Section: Discussionsupporting

confidence: 82%

Salinity modulates thermotolerance, energy metabolism and stress response in amphipodsGammarus lacustris

Vereshchagina

Lubyaga

Shatilina

et al. 2016

PeerJ

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Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was observed, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress.

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Section: Discussionsupporting

confidence: 82%

Salinity modulates thermotolerance, energy metabolism and stress response in amphipodsGammarus lacustris

Vereshchagina

Lubyaga

Shatilina

et al. 2016

PeerJ

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“…In ecotoxicology, experiments with gammarids as biological models were frequently conducted on males only (Naylor et al 1989;Pantani et al 1997;Maltby et al 2002;Felten et al 2008;Xuereb et al 2009) or gender where not distinguished (Timofeyev et al 2006(Timofeyev et al , 2008Adam et al 2009;Alonso et al 2010). Our results reveal a gender-based sensitivity probably due to physiological properties corresponding with gender.…”

Section: Discussionmentioning

confidence: 49%

Effects of sublethal copper exposure on two gammarid species: which is the best competitor?

Sroda¹,

Cossu-Leguille²

2010

Ecotoxicology

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Biomarker responses in organisms exposed to copper were examined by comparing two gammarid species, Gammarus roeseli and Dikerogammarus villosus, based on gender. G. roeseli specimens were exposed to 20 μg/L of copper for 6, 12, 24 and 48-h periods, while D. villosus were exposed to 20 and 30 μg/L of copper for 12, 48 and 72 h. Males and females of each species were exposed separately and biomarker measurements were performed for each species and gender. The selected biomarkers were antioxidant enzymes as total glutathione peroxidase (GPxtot), selenium-dependent glutathione peroxidase (SeGPx), and catalase activities. Malondialdehyde level (MDA) was measured as a biomarker of toxic effect. Energy reserves were evaluated by means of lipid, glycogen and protein levels. For both species and gender, antioxidant enzyme activities were weakly modified by copper exposure and differences were transient. MDA levels were increased in both species and genders in exposed animals compared to controls, when energy reserves were decreased. G. roeseli was more rapidly overwhelmed by copper toxicity while the first response of D. villosus was the mobilization of its energetic content. D. Villosus probably has specific physiological properties that enable it to cope with copper toxicity and thus become the best competitor.

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“…A similar study involving Potamonautes warreni found that MTLP bound Cd more effectively than Cu and Zn. The biochemical responses of amphipod species were examined by exposing them to CdCl 2 and examining various biochemical parameters (Timofeyev et al 2008). The results showed that antioxidant enzyme activity and stress protein (HsPs) contents increased depending on the Cd concentration.…”

Section: Antioxidative Enzymesmentioning

confidence: 99%

Evaluation of effects of exposure conditions on the biological responses of Gammarus pulex exposed to cadmium

Kar

2013

Int. J. Environ. Sci. Technol.

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This study was conducted to determine the single and combined effects of cadmium (Cd) exposure at different concentrations and durations on biological responses in Gammarus pulex. The animals were exposed to 4, 8, 16 or 32 lg l -1 of CdCl 2 for 24, 48, 72 or 96 h, respectively. Cd accumulation, protein content, malondialdehyde content (MDA) and three antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were studied. The results were analyzed with two-way analysis of variance (two-way ANOVA) to assess the significance of the effects of exposure concentration and duration and their combined effects. The highest accumulation of Cd was found as 15.07 lg g -1 at 32 lg l -1 and 96-h Cd exposure. There was a significant correlation between accumulated Cd concentration and MDA and protein content and GPx activity. Although there was no significant synergistic effect of duration and concentration on Cd accumulation, a significant synergy of duration and concentration was observed in all other studied parameters. Interestingly, with the exception of GPx activity, the effect of concentration was greater than the effect of duration for all studied parameters. GPx activity showed that duration had a greater effect than concentration. Additionally, both SOD and GPx are more effective than CAT during Cd stress. The results of this study may be useful for understanding the relationship between organisms and environmental toxins.

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Evaluation of biochemical responses in Palearctic and Lake Baikal endemic amphipod species exposed to CdCl2

Cited by 24 publications

References 40 publications

Salinity modulates thermotolerance, energy metabolism and stress response in amphipodsGammarus lacustris

Salinity modulates thermotolerance, energy metabolism and stress response in amphipodsGammarus lacustris

Effects of sublethal copper exposure on two gammarid species: which is the best competitor?

Evaluation of effects of exposure conditions on the biological responses of Gammarus pulex exposed to cadmium

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