Evaluation of biocontrol efficiency of different Bacillus preparations and field application methods against Phytophthora blight of bell pepper

Jiang, Zhiqiang; Guo, Yin; Li, Shimo; Qi, Hong-Ying; Guo, Jianhua

doi:10.1016/j.biocontrol.2005.10.012

Cited by 64 publications

(19 citation statements)

References 25 publications

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“…These hydrolases contribute to degradation of fungal cell walls (Korsten et al 1993;Paulitz and Belanger 2001;Helistö et al 2001). Species of Bacillus have been shown to be effective and are commercially applied as biological control agents against fungal and nematode pathogens and pests (Weller 1988;Stabb et al 1994;Backman et al 1994;Tang 1994;Paulitz and Bélanger 2001;Berger et al 1996;Jiang et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant

Jiang

et al. 2007

BioControl

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A chitinase-secreting strain CH2 was one of 353 strains isolated from rhizosphere of eggplant. Based on 16S rDNA sequence alignment and several biochemical and physiological characteristics, the strain was identified to be of Bacillus cereus. On chitinAyers (CA) medium, the strain secreted chitinase. Evaluation of its activity, combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), showed it to be a 15.0-KD chitinase. On glass slides, germination of the fungal spores was effectively suppressed by the bacterial suspension, supernatant from the suspension, and 0.005% solution of chitinase extracted from the strain CH2. The optimum pH for chitinase was 7.1 and optimum temperature was 40°C. At that temperature, high-level chitinolytic activity was retained for 10 days. In greenhouse experiments, suspension of the cells of the CH2 strain reduced the severity of Verticillium wilt on eggplant by 69.69%, its supernatant by 54.04%, and the enzyme diluted to 0.01% strength by 53.13% in 14 days. Strain CH2 and its chitinase have good commercial potential in controlling Verticillium wilt.

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Section: Introductionmentioning

confidence: 99%

Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant

Jiang

et al. 2007

BioControl

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“…Many BCAs are used against Phytophthora spp in the soil (Smith, Wilcox, and Harman 1990;Yuan and Crawford 1995;Berger et al 1996;Utkhede, Sholberg, and Smirle 2001;Jiang, Guo, Li, Qi, and Guo 2006), and a foliar treatment for Phytophthora is something that has not been tested as extensively. Biocontrol treatments might perform better in the soil and leaf litter against P. ramorum inoculum if they are indigenous to these environments.…”

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confidence: 99%

Evaluation of several commercial biocontrol products on European and North American populations ofPhytophthora ramorum

Elliott

Shamoun

Sumampong

et al. 2009

Biocontrol Science and Technology

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Five commercially available biological control products were tested in vitro with seven isolates of Phytophthora ramorum from North American (NA1, NA2), and European (EU1) populations. The in vitro tests included dual culture methods and detached leaf assays on wounded Rhododendron and Camellia leaves. Variability in response to biocontrol agents among isolates of P. ramorum from North American and European populations was examined. In dual culture tests, both Bacillus subtilis products (Companion † and Serenade † ) resulted in better inhibition of the NA1 group than NA2 and EU1. Actinovate † (Streptomyces lydicus) was the least effective of the three bacterial biocontrol agents and there was no difference in percent inhibition among P. ramorum lineages. Two products containing Trichoderma spp. were tested: Plant Helper † (T. atroviride) caused 100% inhibition of all lineages of P. ramorum, while SoilGard TM (T. virens) was only about 30% effective. There was great variability among P. ramorum isolates in their response to biocontrol agents. All treatments reduced P. ramorum lesion size on both Rhododendron and Camellia. Combined treatments of Actinovate † with one other BCA did not perform as well as either treatment used individually. Best results were obtained with Serenade † on Rhododendron and Camellia foliage, especially against the NA1 group. Lack of a linear relationship between percent inhibition of P. ramorum by BCAs in vitro and foliar treatments on detached Rhododendron and Camellia leaves indicates that in vitro testing is a poor predictor of BCA performance on plant material.

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“…This disease is very difficult to control, since the pathogen disperses rapidly via splashing rain as well as flowing irrigation and surface water. Thus, potato plants can be destroyed rapidly in 3–5 days under the condition of wet soils above 18°C and prolonged wet periods with air temperatures ranging from 24 to 29 °C (Jiang, Guo, Li, Qi, & Guo, ). In field experiment, even though all treated groups were finally infected, Bacillus treatment effectively suppressed plant infection when compared to nontreatment group during early outbreak periods (Day 43).…”

Section: Resultsmentioning

confidence: 99%

Potential of anaerobic digestate of dairy manure in suppressing soil‐borne plant disease

Pan

Andriamanohiarisoamanana

et al. 2018

Animal Science Journal

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Frequent use of pesticides to control soil-borne plant disease leads to environmental pollution and the development of pesticide resistance in phytopathogens. Soil amendment is considered to have the potential of suppressing plant disease because of its biological properties. However, information on anaerobic digestate is limited. In this study, potential of antagonistic activities of anaerobic digestate against phytopathogens were investigated by detecting the amounts of antagonistic bacteria (Bacillus and Pseudomonas) in anaerobic digestates of dairy manure. The results showed that anaerobic digestion increased the total amounts of Bacillus and Pseudomonas in digestate. Bacillus suppressed growth of phytopathogens, while Pseudomonas did not show any antagonistic activities. These results indicated that Bacillus was an effective antagonistic bacterium in digestate against phytopathogens. Furthermore, two selected isolates, B11 (Bacillus subtilis) and B59 (Bacillus licheniformis), were applied in field experiments and showed significant reduction in percent infection of potato late blight (Phytophthora infestans). These results demonstrate the benefits of digestate in suppressing soil-borne plant diseases caused by antagonistic bacteria.

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Evaluation of biocontrol efficiency of different Bacillus preparations and field application methods against Phytophthora blight of bell pepper

Cited by 64 publications

References 25 publications

Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant

Characterization of chitinase secreted by Bacillus cereus strain CH2 and evaluation of its efficacy against Verticillium wilt of eggplant

Evaluation of several commercial biocontrol products on European and North American populations ofPhytophthora ramorum

Potential of anaerobic digestate of dairy manure in suppressing soil‐borne plant disease

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