Evaluation of biofilm production and characterization of genes encoding type III secretion system among Pseudomonas aeruginosa isolated from burn patients

Jabalameli, Fereshteh; Mirsalehian, Akbar; Khoramian, Babak; Aligholi, Marzieh; Khoramrooz, Seyed Sajjad; Asadollahi, Parisa; Taherikalani, Morovat; Emaneini, Mohammad

doi:10.1016/j.burns.2012.07.030

Cited by 90 publications

(80 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In another study that was conducted by Agnello et al, the prevalence of exoU, exoS , exoU/exoS or other exotoxin genes were 38%, 56% and 8%; respectively (4). Jabalameli et al, were reported the frequency of exoS, exoU and exoU/exoS in burn isolates 29%, 64.5% and 1%; respectively (20), which their finds was conducted with ours. In a survey that conducted by Lakshmi Priya et al, all of the isolates were carried one of the exoU, exoS or exoU/exoS genes.…”

Section: Discussionsupporting

confidence: 72%

Detection of Exotoxins and Antimicrobial Susceptibility Pattern in Clinical Pseudomonas Aeruginosa Isolates

Mohamad¹,

Rostami²,

Zamanzad³

et al. 2017

Avicenna J Clin Microb Infec

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 72%

Detection of Exotoxins and Antimicrobial Susceptibility Pattern in Clinical Pseudomonas Aeruginosa Isolates

Mohamad¹,

Rostami²,

Zamanzad³

et al. 2017

Avicenna J Clin Microb Infec

View full text Add to dashboard Cite

show abstract

“…To assess the presence of the MBL genes (bla IMP , bla VIM , bla SIM , bla GIM and bla SPM ) in P. aeruginosa strains, a multiplex PCR was performed, as described previously (Woodford, 2010). The presence of the TTSS virulence genes (exoS, exoY, exoT and exoU) was deterrmined by simplex PCR, as described previously (Jabalameli et al, 2012); and to assess the presence of the virulence factors (algD, toxA and lasB) a simplex PCR was performed, as described by Lanotte et al (2004). All PCRs were carried out in a Mastercycler Personal (Eppendorf).…”

Section: Methodsmentioning

confidence: 99%

Molecular epidemiological survey of the quinolone- and carbapenem-resistant genotype and its association with the type III secretion system in Pseudomonas aeruginosa

Ferreira

Dantas

Faria

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

This study evaluated the predictors of mortality and the impact of inappropriate therapy on the outcomes of patients with bacteraemia and ventilator-associated pneumonia (VAP). Additionally, we evaluated the correlation of the type III secretion system (TTSS) effector genotype with resistance to carbapenems and fluoroquinolones, mutations in the quinolone resistancedetermining regions (QRDRs), metallo-b-lactamase and virulence factors. A retrospective cohort was conducted at a tertiary hospital in patients with multidrug-resistant (MDR) P. aeruginosa bacteraemia (157 patients) and VAP (60 patients). The genes for bla IMP , bla VIM , bla SIM , bla GIM and bla SPM and virulence genes (exoT, exoS, exoY, exoU, lasB, algD and toxA) were detected; sequencing was conducted for QRDR genes on fluoroquinolone-resistant strains. The multivariate analyses showed that the predictors independently associated with death in patients with bacteraemia were cancer and inappropriate therapy. Carbapenem resistance was more frequent among strains causing VAP (53.3 %), and in blood we observed the bla SPM genotype (66.6 %) and bla VIM genotype (33.3 %). The exoS gene was found in all isolates, whilst the frequency was low for exoU (9.4 %). Substitution of threonine to isoleucine at position 83 in gyrA was the most frequent mutation among fluoroquinolone-resistant strains. Our study showed a mutation at position 91 in the parC gene (Glu91Lys) associated with a mutation in gyrA (Thre83Ile) in a strain of extensively drug-resistant P. aeruginosa, with the exoT + exoS + exoU + genotype, that has not yet been described in Brazil to the best of our knowledge. This comprehensive analysis of resistance mechanisms to carbapenem and fluoroquinolones and their association with TTSS virulence genes, covering MDR P. aeruginosa in Brazil, is the largest reported to date. INTRODUCTIONSevere infections such as bloodstream infection and ventilator-associated pneumonia (VAP) due to multidrugresistant (MDR) Pseudomonas aeruginosa result in greater morbidity and mortality, longer hospitalization and higher cost than infections caused by susceptible bacteria (Morales et al., 2012; Suárez et al., 2010;Voor In't Holt et al., 2014), and aspects related to epidemiology and the outcome of patients with these infections may change, resulting in high rates of resistance and as consequence difficulties in treatment (Kang et al., 2005; Lodise et al., 2007).Today, the emergence of MDR P. aeruginosa is a global problem (Sader et al., 2001; Van der Bij et al., 2011, resulting in the ability of this pathogen to develop resistance to almost all available antibiotics, either by selection of mutations in chromosomal genes or from horizontal gene transfer (Breidenstein et al., 2011). In Brazil this problem is hih5ghly significant and some studies show a very high density of antibiotic use, especially of carbapenems and fluoroquinolones (Moreira et al., 2013;Porto et al., 2013). The resistance of P. aeruginosa to carbapenems is higher than 60 % in some Brazilian hospitals ...

show abstract

“…There are several virulence factors in UPEC isolates that increase their ability to colonize and persist in the urogenital tract. An important virulence factor in various pathogenic bacteria causing human UTI is the biofilm formation according to the study conducted by Jabalameli et al 17 Urinary tract infections are among the most common bacterial diseases worldwide which involve (infects) about 250 million people in developing countries annually. 18,19 Uropathogenic E. coli on its own account for 70-90% of the UTI infections and their susceptibility patterns against different antibiotics vary in different geographical regions.…”

Section: Discussionmentioning

confidence: 99%

Detection of biofilm formation by Escherichia coli with its antibiogram profile

Risal¹,

Shrestha²,

Kunwar³

et al. 2018

Int J Community Med Public Health

View full text Add to dashboard Cite

Background: In urinary tract infections, an important role is played by bacterial biofilms which are responsible for persistence infections together with the antimicrobial resistance. Higher resistance can be seen in biofilm forming uropathogens in comparison with free-floating bacteria. So, the present study was performed with a goal to find the prevalence of biofilm formation and also the antimicrobial resistant pattern of uropathogens.Methods: A descriptive method was conducted at Modern Technical College, Sanepa, Lalitpur in samples isolated from UTI suspected patients. The overall duration of this study was approximately 3 months. Total of 50 isolated E. coli was tested for biofilm formation and antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method on Mueller Hinton agar as per CLSI guidelines.Results: From the 50 isolates of E. coli, 32 were biofilm producers (3 strong and 29 moderate) and 18 were weak/non-biofilm producers. Among the biofilm producers, cefotaxime was more resistant in 20 of the isolates followed by ceftriaxone in 16 and amoxyclav in 13, whereas amikacin was least resistant in 2 of the isolates.Conclusions: Among the isolated E. coli, biofilm-forming isolates showed higher antimicrobial resistance as compared to the non-biofilm producer. Thus, uropathogen should be routinely screened for biofilm formation.

show abstract

Evaluation of biofilm production and characterization of genes encoding type III secretion system among Pseudomonas aeruginosa isolated from burn patients

Cited by 90 publications

References 25 publications

Detection of Exotoxins and Antimicrobial Susceptibility Pattern in Clinical Pseudomonas Aeruginosa Isolates

Detection of Exotoxins and Antimicrobial Susceptibility Pattern in Clinical Pseudomonas Aeruginosa Isolates

Molecular epidemiological survey of the quinolone- and carbapenem-resistant genotype and its association with the type III secretion system in Pseudomonas aeruginosa

Detection of biofilm formation by Escherichia coli with its antibiogram profile

Contact Info

Product

Resources

About