Evaluation of Biofuel Cells with Hemoglobin as Cathodic Electrocatalysts for Hydrogen Peroxide Reduction on Bare Indium-Tin-Oxide Electrodes

Ayato, Yusuke; Matsuda, Naoki

doi:10.3390/en7010001

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…Great efforts have been mainly conducted by a mediated electron transfer including modified or immobilized electrodes in the Hb electrochemistry (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). On the other hand, we have successfully observed direct electron transfer characteristics and the electrocatalysis for H 2 O 2 of Hb and O 2 reduction by red blood cells (RBCs) on bare indium-tin oxide (ITO) electrodes (16,(31)(32)(33)(34). No strong denaturation of Hb protein structure was observed on the ITO electrodes (32).…”

Section: Introductionmentioning

confidence: 99%

Oxygen Reduction Reaction of Hemoglobin on Indium-Tin-Oxide Electrodes and Its Application to Biofuel Cell Cathode

Ayato¹,

Sugimoto²

2015

ECS Trans.

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Direct electron transfer characteristics of hemoglobin (Hb) molecules were enhanced on indium-tin oxide (ITO) electrodes synthesized by using a dip-coating solution with a molar ratio of In:Sn = 1:1. The quantity of electrochemically active Hb was estimated to be 222 pmol cm-2 from cyclic voltammetry in a pH 7.4 phosphate buffered saline (PBS) solution. In addition, the Hb exhibited oxygen reduction reaction (ORR) activity in O2 saturated PBS solution at negative potential range from around -0.15 V vs. Ag|AgCl|KCl(satd.). The ORR current reached to 90 mA cm-2 at -0.45 V vs. Ag|AgCl|KCl(satd.). A single cell with the O2|Hb|ITO cathode showed an open circuit voltage of ca. 0.72 V and a maximum power density of ca. 1.1 mW cm-2.

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Section: Introductionmentioning

confidence: 99%

Oxygen Reduction Reaction of Hemoglobin on Indium-Tin-Oxide Electrodes and Its Application to Biofuel Cell Cathode

Ayato¹,

Sugimoto²

2015

ECS Trans.

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“…With the development of molecular biology and protein design techniques, these proteins have found industrial applications. Hemeproteins can be used: as biocatalysts in drug production, in biological remediation, biosensor applications, and biofuel cells [29]- [33]. In order to increase the widespread use of hemeproteins in these areas, it is necessary to produce a high amounts of heme bound (holo-) hemeproteins [34], [35].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Hnox Protein Production in Escherichia Coli

Sürmeli¹

2017

Cumhuriyet Science Journal

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Hemeproteins carry a variety of different functions in organisms ranging from steroid biosynthesis to respiration, signaling to drug metabolism. In industry, hemeproteins are used for production of drugs such as: pravastatin for lowering cholesterol, progesterone for hormonal treatment of cancers of uterus and cervix, and cortisone used against allergy and inflammation. Hemeproteins can also be used in drug development and biological remediation. The industrial applications of hemeproteins will expand with development of molecular biology and protein design techniques. One of the obstacles to the widespread use of hemeproteins is difficulties in production of high levels of heme bound protein. This study aims to maximize the amount of heme cofactor bound hemeprotein produced. Here, three important factors affecting hemeprotein production in bacteria are examined: induction by isopropyl β-D-1-thiogalactopyranoside (IPTG), δ-aminolevulinic acid (ALA), precursor for heme biosynthesis, and expression temperature. Effects of these factors on production of thermophilic hemeprotein TtHNOX are investigated. Since ALA is an expensive molecule, optimization of the amount of ALA used is important. The most suitable conditions to produce TtHNOX is at low temperature, 0.5 mM IPTG and 1 mM ALA. This study concludes that ALA concentration and expression temperature are important in production of heme bound hemeproteins.

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“…Another challenge in the design of biofuel cells is the choice of the electrode conducting material, which is important for effective operation of enzyme biocatalytic systems. While many biofuel cells were based on traditional electrode materials (gold , indium tin oxide , glassy carbon , carbon paste , etc. ), recently the attention has been given to highly porous carbon‐based materials using, for example, carbon papers (made of carbon fibers or carbon nanotubes ,).…”

Section: Introductionmentioning

confidence: 99%

A Biofuel Cell Based on Biocatalytic Reactions of Lactate on Both Anode and Cathode Electrodes – Extracting Electrical Power from Human Sweat

2017

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Electrodes composed of carbon fibers were modified with graphene nano‐sheets in order to increase their surface area and facilitate electrochemical reactions. Electrocatalytic species, such as Meldola's blue (MB) and hemin were immobilized on the graphene surface due to their π‐π stacking and then used for electrocatalytic oxidation of NADH and reduction of H2O2, respectively. Further modification of these electrodes with enzymes producing NADH and H2O2 in situ (lactate dehydrogenase, LDH, and lactate oxidase, LOx, respectively), allowed assembling of a biofuel cell operating in the presence of lactate, oxygen and NAD+. The cathode of the biofuel cell required lactate and O2 for its operation, while the anode operated in the presence of lactate and NAD+. Notably, both bioelectrocatalytic electrodes operated in the presence of lactate, one producing H2O2 in the reaction catalyzed by LOx in the presence of O2, second producing NADH in the reaction catalyzed by LDH in the presence of NAD+. Both reactions were performed in the biofuel cell without separation of the cathodic and anodic solutions and with no need of a membrane. The biofuel cell was tested in solutions mimicking human sweat and then in real human sweat samples, demonstrating substantial power release being able to activate electronic devices.

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Evaluation of Biofuel Cells with Hemoglobin as Cathodic Electrocatalysts for Hydrogen Peroxide Reduction on Bare Indium-Tin-Oxide Electrodes

Cited by 4 publications

References 43 publications

Oxygen Reduction Reaction of Hemoglobin on Indium-Tin-Oxide Electrodes and Its Application to Biofuel Cell Cathode

Oxygen Reduction Reaction of Hemoglobin on Indium-Tin-Oxide Electrodes and Its Application to Biofuel Cell Cathode

Optimization of Hnox Protein Production in Escherichia Coli

A Biofuel Cell Based on Biocatalytic Reactions of Lactate on Both Anode and Cathode Electrodes – Extracting Electrical Power from Human Sweat

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