Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Çakır, Özgür; Pekmez, Murat; Cepni, Elif; Candar, Bilgin; Fidan, Kerem

doi:10.1590/fst.2014.0060

Cited by 35 publications

(19 citation statements)

References 34 publications

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“…Bcl-2 family of genes plays a critical role in the regulation of the apoptotic process initiated at the mitochondria [ 39 ] and governed the mitochondrial outer membrane permeabilization [ 40 ]. Given that FAA has been identified as inducers of apoptosis, we sought to detect the Bcl-2 mRNA level in MCF-7 and MDA-MB-231 after treatment with FAA.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Proapoptotic Effects of Allium atroviolaceum Flower Extract by Modulating Cell Cycle Arrest and Caspase‐Dependent and p53‐Independent Pathway in Breast Cancer Cell Lines

Khazaei

Hamid

Ramachandran

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Breast cancer is the second leading cause of cancer death among women and despite significant advances in therapy, it remains a critical health problem worldwide. Allium atroviolaceum is an herbaceous plant, with limited information about the therapeutic capability. We aimed to study the anticancer effect of flower extract and the mechanisms of action in MCF-7 and MDA-MB-231. The extract inhibits the proliferation of the cells in a time- and dose-dependent manner. The underlying mechanism involved the stimulation of S and G2/M phase arrest in MCF-7 and S phase arrest in MDA-MB-231 associated with decreased level of Cdk1, in a p53-independent pathway. Furthermore, the extract induces apoptosis in both cell lines, as indicated by the percentage of sub-G0 population, the morphological changes observed by phase contrast and fluorescent microscopy, and increase in Annexin-V-positive cells. The apoptosis induction was related to downregulation of Bcl-2 and also likely to be caspase-dependent. Moreover, the combination of the extract and tamoxifen exhibits synergistic effect, suggesting that it can complement current chemotherapy. LC-MS analysis displayed 17 major compounds in the extract which might be responsible for the observed effects. Overall, this study demonstrates the potential applications of Allium atroviolaceum extract as an anticancer drug for breast cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Proapoptotic Effects of Allium atroviolaceum Flower Extract by Modulating Cell Cycle Arrest and Caspase‐Dependent and p53‐Independent Pathway in Breast Cancer Cell Lines

Khazaei

Hamid

Ramachandran

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Studies on the human colon adenocarcinoma cell line (HT-29), human hepatoma cell line (Hep3B), human breast adenocarcinoma cell line (MCF-7), human neuroblastoma cell line (SH-SY5Y), human osteosarcoma cell line (SaOS-2), and human prostate adenocarcinoma cell line (LNCap) reported promising IC 50 values of peruviana fruits (Antalya, Turkey) after 48 hours for SaOS-2, HT-29, Hep3B, and SH-SY5Y cells of 15.44, 40.79, 24.92, and 44.24 μg/mL. 36 Studies performed on fruits from other species have been accomplished using murine fibroblasts (L929) 37 and human cervical cancer cells (HeLa). 38 …”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells

Mier-Giraldo

Díaz

Delgado

et al. 2017

J Evid Based Complementary Altern Med

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It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts (P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC50) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.

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“…Physalis peruviana is a medicinal plant widely used in traditional medicine to treat diseases such as malaria, asthma, hepatitis, dermatitis, cancer and rheumatism. It has antispasmodic, diuretic, antiseptic, sedative, analgesic, antioxidant, antifungal, antibacterial, anti-inflammatory, cataract-cleansing, antidiabetic and antiparasitic properties [ 23 , 24 , 25 , 26 , 27 , 28 ]. It has been described as a good source of nutrients and bioactive compounds, β-carotene and vitamins A and C, and minerals including K, Mg and Cu, with moderate fibre content, phenolic compounds and low levels of calories [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants

et al. 2020

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The extraction of secondary metabolites by water, MeOH:water (8:2) containing NaF, methanol, ethanol and acetone (all of them diluted (7:3) in water)from the different parts (leaves, flowers, stems and roots) of Passiflora caerulea L., Physalis peruviana L. and Solanum muricatum Aiton via decoction and maceration methods was studied. The highest extraction yields were recorded by methanol for decoction and acetone for maceration. The total polyphenol content (TPC) obtained by decoction had the highest TPC contents, and MeOH containing NaF was the best solvent for the extraction of TPC. Maceration was suitable for flavonoid extractions, with ethanol and acetone being the best solvents. In general, the highest levels of TPC and flavonoids were obtained from Passiflora leaves regardless of the solvent or extraction method applied. Furthermore, the roots of Physalis and Solanum showed important levels of these compounds in consonance with the total antioxidant activity (TAA) evaluated in the different organs of the plant in the three species. In this study, the solvents and extraction methods applied were tools that determined significantly the level of extraction of bioactive compounds, showing a different impact on plant organs for each medicinal species studied.

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Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Cited by 35 publications

References 34 publications

Cytotoxicity and Proapoptotic Effects of Allium atroviolaceum Flower Extract by Modulating Cell Cycle Arrest and Caspase‐Dependent and p53‐Independent Pathway in Breast Cancer Cell Lines

Cytotoxicity and Proapoptotic Effects of Allium atroviolaceum Flower Extract by Modulating Cell Cycle Arrest and Caspase‐Dependent and p53‐Independent Pathway in Breast Cancer Cell Lines

Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells

Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants

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