Evaluation of biological effectiveness of amino acid mixture as potential stimulator of synthetic processes in skeletal muscles

Abstract

Background. The ability of certain amino acids to stimulate anabolic processes in skeletal muscles has been proved by fundamental research, which makes it important to search for effective agents based on amino acids for stimulation of synthetic processes in skeletal muscles.
 Aim. To study the effect of oral administration of the original amino acid mixture (Larginine, Lmethionine, Lleucine, Lisoleucine) on protein, lipid and carbohydrate metabolism in skeletal muscles and liver of CBA male mice.
 Material and Methods. Two series of experiments were performed. In the first series (n=36), the animals were divided into three groups. In group 1 (n = 12), the mice received a diet balanced in protein and carbohydrates for two months. The animals of group 2 (n=12) were kept on a carbohydrate, proteindepleted isocaloric diet, in which wheat gliadin served as the protein source. Mice of group 3 (n=12) were kept on a diet similar to the second group, in which deficit of protein was compensated for with the tested mixture of Lamino acids. In the animals of the second series (n=36) acute liver failure was modeled by a single intraperitoneal injection of 20% carbon tetrachloride solution (CTC) in olive oil. Three days after the injection of CTC, all animals of the second series were randomly divided into three groups, depending on the received diet.
 Results. The results of the first series of experiments showed that compensation for protein deficiency with amino acid mixture reliably prevented excessive buildup of glycogen in muscles, led to a decrease in lipids in tissue, and also prevented reduction in the level of muscle protein. The results of the second series of experiments showed that intake of the amino acid mixture prevented loss of protein in muscles and supported the proteinsynthetic function of the liver.
 Conclusion. The study demonstrated that the tested mixture, when taken orally, can prevent disorders of proteincarbohydratelipid ratio in the muscles.