Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Kim, Meyoung Kon; Oh, Sangnam; Lee, Ji Hye; Im, Hosub; Ryu, Yeon Mi; Oh, Eunha; Lee, Joohyun; Lee, Eunil; Sul, Donggeun

doi:10.1038/emm.2004.52

Cited by 27 publications

(16 citation statements)

References 29 publications

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“…Other studies assessing occupational exposure to pollutants have also found higher concentrations of 1-HOP: coke oven and graphite electrode producing plant workers [41,42]; coal liquefaction workers [43]; road pavers [44]; aluminum plant workers [35]; carbon black workers [45]. The same observation has been made in studies assessing 2-NAP concentrations in the urine of workers exposed to different classes of pollutants: charcoal workers [46,47] and emission inspectors [47]. In general, exposure assessments and biomarkers have found differences between the control and exposed populations, suggesting a likely linkage between the class of agent measured by the exposure assessment and the damage detected by biomarker [1].…”

Section: Discussionsupporting

confidence: 58%

Biomonitoring of genotoxic risk of workers exposed to heavy air pollution

Rainho¹,

Corrêa²,

Aiub³

et al. 2015

WIT Transactions on Ecology and the Environment

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Epidemiological studies found an increased risk of cancers in occupations exposed to traffic air pollution. PM2.5 are toxic and can enter into the respiratory tract and circulatory system. PM2.5 can adsorb various substances, such as polycyclic aromatic hydrocarbons (PAHs) nitro-PAHs. The present study was carried out with 15 Rebouças tunnel (Rio de Janeiro, Brazil) workers (exposed group) and 11 healthy men (control group). The participants were informed about the study and asked to sign an informed consent form and to complete a standard questionnaire to obtain necessary data on their lifestyle. Samples of buccal mucosa cells and peripheral blood were evaluated using micronucleus (MN) assay. Urine samples were used to estimate the concentration of 1-hydroxyprene (1-HOP) and 2-naphthol (2-NAP). A significantly higher frequency (10.82) of MN in buccal cells and (4.42) binucleated lymphocytes were detected for the exposed workers. Higher concentrations of 1-HOP (16.47 µmol/mol creatinine) and 2-NAP (6.56 µmol/mol creatinine) were also detected in the exposure group. In conclusion, damage to the genetic material and the high concentrations of metabolites of PAHs detected in the biological samples taken from Rebouças tunnel workers can be related to daily exposure to pollutants in the tunnel.

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Section: Discussionsupporting

confidence: 58%

Biomonitoring of genotoxic risk of workers exposed to heavy air pollution

Rainho¹,

Corrêa²,

Aiub³

et al. 2015

WIT Transactions on Ecology and the Environment

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“…GSTM3, one of the oxidative stress response genes, was upregulated in humans with chloracne (McHale et al, 2007), in waste incineration workers (Kim et al, 2004), and in animal models (Shertzer et al, 1998) following TCDD exposure. Our study identified the "glutathione S-transferase, mu type 3" as an upregulated protein spot, the intensity of which in the 125-ng/kg/day group was around 2.5-fold higher than for the control.…”

Section: Discussionmentioning

confidence: 99%

“…The TCDD-AhR complex is subsequently translocated to the nucleus of the cell, forming a heterodimer with the AhR nuclear translocator (ARNT). The TCDD-AhR-ARNT complex adheres to dioxin-responsive elements (DRE), inducing the transcription of a number of genes (Tang et al, 2008), and alters the expression of a wide range of proteins (Kim et al, 2004).…”

Section: 378-tetrachlorodibenzo-p-dioxin (Tcdd) Is a Ubiquitouslymentioning

confidence: 99%

Proteomic Analysis of the Rat Ovary Following Chronic Low-Dose Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)

Chen

et al. 2009

Journal of Toxicology and Environmental Health, Part A

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“…The previous studies in animal models affirmed a sustained oxidative stress response in mice following TCDD exposure. Another report on global gene expression (1152 genes) in waste incineration workers occupationally exposed to dioxin, showed upregulation of five genes related to oxidative stress (Kim et al, 2004;McHale et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Expression of AhR, CYP1A1, GSTA1, c-fos and TGF-α in skin lesions from dioxin-exposed humans with chloracne

et al. 2008

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Occupational exposure to certain polychlorinated aromatic hydrocarbons such as dioxins has been suggested to cause chloracne which is a kind of skin disease. The molecular mechanisms of dioxin-mediated chloracne have not been clarified. It is possible that dioxins contribute to the pathogenesis through activation of aryl-hydrocarbon receptor (AhR)-mediated transcription and downstream genes such as CYP1A1, GSTA1 and TGF-α. The study on genes was through chloracne lesional skin, which has rarely been reported on previously. The expression levels of key genes, such as AhR, CYP1A1, GSTA1, c-fos and TGF-α in human epidermal tissue of chloracne cases and controls were detected by real-time PCR. Compared with controls, AhR, CYP1A1, GSTA1 and c-fos transactivations were significantly induced in the skins of chloracne patients who had long-term exposure to dioxins and dibenzofuranes. The TGF-␣ mRNA content of epidermal tissue was increased, but not significantly compared with controls. The study demonstrates that constitutive activation of the AhR pathway is probably a prerequisite of chloracne pathogenesis. The changes of genes expression may disturb normal proliferation and differentiation of human epidermis cells, and then lead to chloracne.

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Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins

Abstract: Abbreviations: 1-OHP, 1-hydroxy pyrene; 2-DE, second dimensional electrophoresis; GC/MS, gas chromatography/mass spectrometry; HPLC, High performance liquids chromatography; PAHs, polycyclic aromatic hydrocarbons

Cited by 27 publications

References 29 publications

Biomonitoring of genotoxic risk of workers exposed to heavy air pollution

Biomonitoring of genotoxic risk of workers exposed to heavy air pollution

Proteomic Analysis of the Rat Ovary Following Chronic Low-Dose Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)

Expression of AhR, CYP1A1, GSTA1, c-fos and TGF-α in skin lesions from dioxin-exposed humans with chloracne

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