This article presents studies on the evaluation of the impact of surface modification of cotton, viscose, and polyester fabrics using three techniques (flocking, layer by layer, and screen printing) with materials with electrically conductive properties on their structural, biophysical, and conductive properties. Each tested fabric is characterized by specific biophysical properties. which can be disturbed by various modification methods, therefore, the following tests were carried out in the article: optical microscopy, micro-computed tomography, guarded perspiration heating plate, air permeability, sorption and electrical conductivity tester. The use of screen printing increased the thermal resistance of the cotton woven fabric by 119%, the polyester woven fabric by 156%, and the viscose fabric by 261%. The smallest changes in thermal resistance compared to unmodified textiles were observed in layer by layer modified fabrics and are as follows: −15% (cotton woven fabric), +77% (PES woven fabric), and +80% (viscose woven fabric).