Evaluation of blood collection filter papers for HIV-1 DNA PCR

Masciotra, Silvina; Khamadi, Samoel; Bilé, Ebi; Puren, Adrian; Fonjungo, Peter N.; Nguyen, Shon; Girma, Mulu; Downing, Robert; Ramos, Artur; Subbarao, Shambavi; Ellenberger, Dennis

doi:10.1016/j.jcv.2012.06.010

Cited by 17 publications

(13 citation statements)

References 41 publications

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“…These filter card systems are highly uniform and have very similar absorption characteristics so that analytical results obtained from capillary blood prepared on either of them do not differ by more than 4% -5% 28 . Not surprisingly, Masciotra and co-workers 29 therefore detected HIV-1 RNA equally well with a qualitative assay after elution from blood collection cards from different sources. Given these data, it can be virtually excluded that any of the discrepancies observed when testing coupled serum/DBS pairs for markers of HBV, HCV and HIV infections 17 was caused by the choice of the filter card alone.…”

Section: Parametersmentioning

confidence: 82%

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Grüner

Stambouli

Roß

2015

JoVE

125

110

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The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since then, DBS testing for decades has remained predominantly focused on the diagnosis of infectious diseases especially in resourcelimited settings or the systematic screening of newborns for inherited metabolic disorders and only recently have a variety of new and innovative DBS applications begun to emerge. For many years, pre-analytical variables were only inappropriately considered in the field of DBS testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the preparation and processing of DBS for their final analysis has not been standardized. Given this background, a comprehensive step-by-step protocol, which covers al the essential phases, is proposed, i.e., collection of blood; preparation of blood spots; drying of blood spots; storage and transportation of DBS; elution of DBS, and finally analyses of DBS eluates. The effectiveness of this protocol was first evaluated with 1,762 coupled serum/DBS pairs for detecting markers of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus infections on an automated analytical platform. In a second step, the protocol was utilized during a pilot study, which was conducted on active drug users in the German cities of Berlin and Essen.

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Section: Parametersmentioning

confidence: 82%

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Grüner

Stambouli

Roß

2015

JoVE

125

110

View full text Add to dashboard Cite

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“…To that end, dried blood spots (DBS) have emerged as a convenient route for home blood sampling allowing preservation of dried samples and the application on laboratory-consumer communication by postal services. DBS technology was first used in neonatal screening for inherited metabolic disorders and is extensively used in HIV studies (16)(17) . The use of DBS sampling has been extended to many biochemical parameters, including hormones, cytokines, vitamins and lipids (18)(19)(20) .…”

Section: Personalised Nutrition Based On Personalised Phenotypic Datamentioning

confidence: 99%

The future direction of personalised nutrition: my diet, my phenotype, my genes

Gibney

Walsh

2013

Proc. Nutr. Soc.

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Although personalised nutrition is frequently considered in the context of diet-gene interactions, increasingly, personalised nutrition is seen to exist at three levels. The first is personalised dietary advice using Internet-delivered services, which ultimately will become automated and which will also draw on mobile phone technology. The second level of personalised dietary advice will include phenotypic information on anthropometry, physical activity, clinical parameters and biochemical markers of nutritional status. It remains possible that in addition to personalised dietary advice based on phenotypic data, advice at that group or metabotype level may be offered where metabotypes are defined by a common metabolic profile. The third level of personalised nutrition will involve the use of genomic data. While the genomic aspect of personalised nutrition is often considered as its main driver, there are significant challenges to translation of data on SNP and diet into personalised advice. The majority of the published data on SNP and diet emanate from observational studies and as such do not offer any cause-effect associations. To achieve this, purpose-designed dietary intervention studies will be needed with subjects recruited according to their genotype.

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“…The LOD information for the Roche CAPCTM Qual is not available to the end user. The 8E5 cells have been used as quality control standards in HIV testing (Burgard et al, 2000; Jani et al, 2012; Garcia et al, 2014) and in a DBS collection filter paper study for HIV-1 DNA PCR (Masciotra et al, 2012). However, no other information was found in the literature about using the 8E5 cells for the LOD determination of other diagnostic assays for early infant detection of HIV.…”

Section: Discussionmentioning

confidence: 99%

Field evaluation of Abbott Real Time HIV-1 Qualitative test for early infant diagnosis using dried blood spots samples in comparison to Roche COBAS Ampliprep/COBAS TaqMan HIV-1 Qual Test in Kenya

Chang

Omuomo

Anyango

et al. 2014

Journal of Virological Methods

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Timely diagnosis and treatment of infants infected with HIV are critical for reducing infant mortality. High-throughput automated diagnostic tests like Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Qual Test (Roche CAPCTM Qual) and the Abbott Real Time HIV-1 Qualitative (Abbott Qualitative) can be used to rapidly expand early infant diagnosis testing services. In this study, the performance characteristics of the Abbott Qualitative were evaluated using two hundred dried blood spots (DBS) samples (100 HIV-1 positive and 100 HIV-1 negative) collected from infants attending the antenatal facilities in Kisumu, Kenya. The Abbott Qualitative results were compared to the diagnostic testing completed using the Roche CAPCTM Qual in Kenya. The sensitivity and specificity of the Abbott Qualitative were 99.0% (95% CI: 95.0–100.0) and 100.0% (95% CI: 96.0–100.0), respectively, and the overall reproducibility was 98.0% (95% CI: 86.0–100.0). The limits of detection for the Abbott Qualitative and Roche CAPCTM Qual were 56.5 and 6.9 copies/mL at 95% CIs (p = 0.005), respectively. The study findings demonstrate that the Abbott Qualitative test is a practical option for timely diagnosis of HIV in infants.

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Evaluation of blood collection filter papers for HIV-1 DNA PCR

Cited by 17 publications

References 41 publications

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

The future direction of personalised nutrition: my diet, my phenotype, my genes

Field evaluation of Abbott Real Time HIV-1 Qualitative test for early infant diagnosis using dried blood spots samples in comparison to Roche COBAS Ampliprep/COBAS TaqMan HIV-1 Qual Test in Kenya

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