Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions

Garg, Rahul; Ramachandran, Krithiga; Jayashree, S.; Agarwal, Reshu; Gupta, Ekta

doi:10.1016/j.jcvp.2022.100111

Cited by 3 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These patients likely took ART inconsistently or had interrupted treatment. DBS has also been used to detect and quantify the respective hepatitis B virus (HBV) [26,[95][96][97] and hepatitis C virus (HCV) [25,27,98,99]. These studies indicated that DBS can provide a very accurate detection of the viruses and be utilised as point-of-care testing for patients.…”

Section: Dbs As a Matrix To Monitor Viral Loadmentioning

confidence: 99%

“…In addition, DBS has proven to be valuable in disease response monitoring (DRM), especially for conditions that require frequent monitoring biomarkers. For example, in the field of infectious diseases, DBS can be used to detect and monitor viral loads or antibodies in individuals infected with human immunodeficiency virus (HIV), hepatitis C or other viral pathogens [21][22][23][24][25][26][27]. With the applications of multi-omic analytical techniques [28,29], DBS can be explored as a platform for both TDM and DRM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dried Blood Spots—A Platform for Therapeutic Drug Monitoring (TDM) and Drug/Disease Response Monitoring (DRM)

Zailani,

2023

Eur J Drug Metab Pharmacokinet

View full text Add to dashboard Cite

This review provides an overview on the current applications of dried blood spots (DBS) as matrices for therapeutic drug (TDM) and drug or disease response monitoring (DRM). Compared with conventional methods using plasma/serum, DBS offers several advantages, including minimally invasiveness, a small blood volume requirement, reduced biohazardous risk, and improved sample stability. Numerous assays utilising DBS for TDM have been reported in the literature over the past decade, covering a wide range of therapeutic drugs. Several factors can affect the accuracy and reliability of the DBS sampling method, including haematocrit (HCT), blood volume, sampling paper and chromatographic effects. It is crucial to evaluate the correlation between DBS concentrations and conventional plasma/serum concentrations, as the latter has traditionally been used for clinical decision. The feasibility of using DBS sampling method as an option for home-based TDM is also discussed. Furthermore, DBS has also been used as a matrix for monitoring the drug or disease responses (DRM) through various approaches such as genotyping, viral load measurement, assessment of inflammatory factors, and more recently, metabolic profiling. Although this research is still in the development stage, advancements in technology are expected to lead to the identification of surrogate biomarkers for drug treatment in DBS and a better understanding of the correlation between DBS drug levels and drug responses. This will make DBS a valuable matrix for TDM and DRM, facilitating the achievement of pharmacokinetic and pharmacodynamic correlations and enabling personalised therapy.

show abstract

Section: Dbs As a Matrix To Monitor Viral Loadmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dried Blood Spots—A Platform for Therapeutic Drug Monitoring (TDM) and Drug/Disease Response Monitoring (DRM)

Zailani,

2023

Eur J Drug Metab Pharmacokinet

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

“…Moreover, sample and analyte stability is severely limited by the presence of water, whereas dried blood microsamples can be stable for months, or even years, and even when dealing with easily degradable analytes, such as DNA, peptides, proteins, etc. (Eshghi et al, 2020;Protti et al, 2021;Garg et al, 2022). Breken et al (2023) have found that, despite the many advantages and minimal invasiveness of the Hem-Col approach, patients with psychiatric disorders undergoing clozapine treatment still prefer classical venepuncture and find it even less painful than fingerpricking.…”

mentioning

confidence: 99%