Evaluation of bone healing following Er:YAG laser ablation in rat calvaria compared with bur drilling

Ohsugi, Yujin; Aoki, Akira; Mizutani, Koji; Katagiri, Seiji; Komaki, Motohiro; Noda, Masahiro; Toru, Takeshi; Kakizaki, Sho; Meinzer, Walter; Izumi, Yuichi

doi:10.1002/jbio.201800245

Cited by 28 publications

(39 citation statements)

References 34 publications

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“…This result is associated with the promoted calcification of osteoblasts by Er:YAG laser irradiation. We also reported that Bglap expression was significantly increased in the granulation tissues of Er:YAG laser-ablated bone defects compared to that of bur-drilled bone defects at 1 week after treatment in rat (Ohsugi et al, 2019). A previous study reported that mineralization of rat calvarial osteoblasts was initiated when cultured in osteogenic induction medium for 3 days, which was earlier than that of mouse osteoblasts on FIGURE 6 | qPCR analysis of mRNA expression in osteoblast-like cells irradiated by low-level Er:YAG laser.…”

Section: Discussionmentioning

confidence: 67%

Effects of Low-Level Er:YAG Laser Irradiation on Proliferation and Calcification of Primary Osteoblast-Like Cells Isolated From Rat Calvaria

Niimi

Ohsugi

Katagiri

et al. 2020

Front. Cell Dev. Biol.

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Several reports have shown that the photo-bio-modulation of cells by various lasers has favorable biological effects. However, the effects of low-level Er:YAG laser irradiation on osteoblasts remain unclear. The purpose of this study was to evaluate the effects of lowlevel Er:YAG laser irradiation on proliferation and osteogenic differentiation of primary osteoblast-like cells isolated from the calvariae of 3-5-day-old Wistar rats. Cells were irradiated by Er:YAG laser at energy fluences of 2.2, 3.3, and 4.3 J/cm 2 , respectively. After irradiation, cell surface temperatures were measured and cell proliferation was evaluated by flow cytometry and CCK-8. Calcification was evaluated by measuring areas of Alizarin red S staining after 7, 14, and 21 days culture in osteoinductive medium. Gene expression in non-irradiated and laser-irradiated cells was evaluated by qPCR at 3, 6, and 12 h, as well as 1, 3, 7, and 14 days after irradiation. Microarray analysis was performed to comprehensively evaluate the gene expression of non-irradiated and irradiated cells at 3.3 J/cm 2 at 6 h after irradiation. No pronounced increase of cell surface temperature was induced by irradiation. Irradiation did not affect osteoblastlike cell proliferation. Osteoblast-like cell calcification was significantly increased 7 days after Er:YAG laser irradiation at 3.3 J/cm 2. Bglap expression was significantly increased in cells irradiated at 3.3 J/cm 2 6 h post-irradiation. Microarray analysis showed that irradiation at 3.3 J/cm 2 caused an upregulation of inflammation-related genes and downregulation of Wisp2. Gene set enrichment analysis also clarified enrichment of inflammation-related and Notch signaling gene sets. In conclusion, low-level Er:YAG laser irradiation at 3.3 J/cm 2 enhanced calcification of primary osteoblast-like cells via enhanced Bglap expression and enriched Notch signaling.

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Section: Discussionmentioning

confidence: 67%

Effects of Low-Level Er:YAG Laser Irradiation on Proliferation and Calcification of Primary Osteoblast-Like Cells Isolated From Rat Calvaria

Niimi

Ohsugi

Katagiri

et al. 2020

Front. Cell Dev. Biol.

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“…Therefore, the increase of inflammation-related genes might be the evidence of suppressed new bone formation in the bur-drilled bone compared to the laser-ablated bone, as reported previously. 17 Furthermore, Hippo signaling pathway was significantly enriched in the laser-ablated bone compared to that of the untreated control bone at 6 hours, although no significant enrichment of the pathway was observed in the bur-drilled bone. Mechanical stress suppresses Sost expression in bone.…”

Section: Discussionmentioning

confidence: 88%

“…Then, serial frontal sections were stained with hematoxylin and eosin, as previously described. 17 The distance from the treated bone surface to the empty lacuna was measured.…”

Section: Histological and Immunohistochemical Analysismentioning

confidence: 99%

“…11,12 Recently, lasers have been applied for bone ablation during periodontal surgery, 13 anticipating advantageous photo-bio-modulation effects other than just mechanical ablation. As shown in many previous papers, [14][15][16] we also reported that bone ablation using Er:YAG laser showed a faster bone healing compared to the conventional bur drilling in rat using 3D micro CT. 17 Based on the above findings, we hypothesized that there was a possibility of Er:YAG laser irradiation modifying Sost expression in bone. 17 However, no study has reported comprehensive and sequential biological response of bone healing with Er:YAG laser ablation.…”

Section: Introductionmentioning

confidence: 97%

“…As shown in many previous papers, [14][15][16] we also reported that bone ablation using Er:YAG laser showed a faster bone healing compared to the conventional bur drilling in rat using 3D micro CT. 17 Based on the above findings, we hypothesized that there was a possibility of Er:YAG laser irradiation modifying Sost expression in bone. 17 However, no study has reported comprehensive and sequential biological response of bone healing with Er:YAG laser ablation. Moreover, bio-stimulation effect by Er:YAG laser on osteocyte is unclear.…”

Section: Introductionmentioning

confidence: 97%

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Laser irradiation decreases sclerostin expression in bone and osteogenic cells

et al. 2020

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Anti‐sclerostin monoclonal antibody romosozumab, a treatment for osteoporosis, reduced vertebral fracture risk and clinical fracture. Laser irradiation triggers various effects, including bio‐stimulation, which can induce beneficial therapeutic effects and biological responses. Originally, we performed in vivo experiments to clarify the mechanism of better bone healing in laser‐ablated bone. Here, we evaluated comprehensive and sequential gene expression in Er:YAG laser‐ablated, bur‐drilled, and nontreated control bones, and found laser irradiation suppressed Sost (coding protein: sclerostin) expression in the bone, possibly via stimulation of mechanotransducers. Surprisingly, bio‐stimulation effect of laser suppressed Sost expression in the primary osteogenic cells. Decreased sclerostin expression after laser irradiation was also validated both in vivo and in vitro. In addition, sequential microarray analysis revealed that the gene expression pattern was clearly different at 24 hours after bone ablation between bur‐drilled and laser‐ablated bones. The Hippo signaling pathway was significantly enriched, whereas inflammation‐related pathways were not affected at 6 hours after the laser ablation, indicating that laser irradiation caused mechanical stimulation. Only bur‐drilled bone showed enriched inflammation‐related gene sets and pathways at 24 hours, not in the laser‐ablated bone. Our study suggests that laser irradiation may become a new treatment modality for osteoporosis, by inhibiting sclerostin expression without inducing inflammation.

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Er:YAG laser‐assisted comprehensive periodontal pocket therapy for residual periodontal pocket treatment: A randomized controlled clinical trial

Aoki

Mizutani

Mikami

et al. 2023

Journal of Periodontology

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BackgroundThis study evaluated the effectiveness of a novel pocket therapy (Er:YAG laser‐assisted comprehensive periodontal pocket therapy [Er‐LCPT]) for residual pocket treatment, compared with conventional mechanical treatment alone, in a randomized controlled clinical trial.MethodsTwo sites in 18 patients having residual periodontal pockets of ≥5 mm depth, extant following initial active therapy, or during supportive therapy, were randomized into two groups in a split mouth design: the control group received scaling and root planing (SRP) by curette, and the test group received Er‐LCPT using curette and laser. With Er‐LCPT, after root debridement, inflamed connective tissue on the inner gingival surface and on the bone surface/within extant bone defects was thoroughly debrided. Furthermore, removal of proximate oral epithelium and coagulation of the blood clot in the pocket entrance were performed with laser. Clinical parameters were evaluated, before and after treatment, through 12 months.ResultsBoth groups showed significant improvements in clinical parameters. With Er‐LCPT, pocket debridement was thoroughly and safely performed, without any adverse side effects and complications, and favorable healing was observed in most of the cases. At 12 months, Er‐LCPT demonstrated significantly higher probing pocket depth reduction (2.78 mm vs. 1.89 mm on average; p = 0.012, Wilcoxon signed‐rank test), clinical attachment gain (1.67 mm vs. 1.06 mm; p = 0.004) as primary outcomes, and reduced BOP value (0.89 vs. 0.56; p = 0.031), compared with SRP alone.ConclusionThe results of this study indicate that Er‐LCPT is more effective for residual pocket treatment, compared with SRP alone.

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Evaluation of bone healing following Er:YAG laser ablation in rat calvaria compared with bur drilling

Cited by 28 publications

References 34 publications

Effects of Low-Level Er:YAG Laser Irradiation on Proliferation and Calcification of Primary Osteoblast-Like Cells Isolated From Rat Calvaria

Effects of Low-Level Er:YAG Laser Irradiation on Proliferation and Calcification of Primary Osteoblast-Like Cells Isolated From Rat Calvaria

Laser irradiation decreases sclerostin expression in bone and osteogenic cells

Er:YAG laser‐assisted comprehensive periodontal pocket therapy for residual periodontal pocket treatment: A randomized controlled clinical trial

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