Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering

Wang, Z H; Zhang, J; Zhang, Q; Gao, Yunkai; Jing, Yan; Zhao, Xiaoyan; Yang, Y Y; Kong, Demin; Zhao, Jingyi; Shi, Yiling; Li, X L

doi:10.4238/gmr.15038431

Cited by 10 publications

(9 citation statements)

References 13 publications

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“…Thrombin acts as an enzyme and converts the fibrogen into fibrin in about 10-60 s [31]. Fibrin glue has been used as a scaffold for a long time to culture a variety of cells and tissue analogues [31][32][33][34]. As a hydrogel, it has also been widely used in nerve tissue engineering to repair peripheral nervous system (PNS) injuries and CNS injuries [35,36].…”

Section: Cell Proliferationmentioning

confidence: 99%

Feasibility of chitosan-alginate (Chi-Alg) hydrogel used as scaffold for neural tissue engineering: a pilot studyin vitro

Wang

Huang

2017

Biotechnology & Biotechnological Equipment

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In tissue engineering, scaffolding plays an important role in accommodating and stimulating new tissue growth. Chitosan and alginate are two widely used natural polymers in tissue engineering. Here, we prepared the chitosan-alginate (Chi-Alg) hydrogel from naturally derived chitosan and alginate polymers. The Fourier-transformed infrared spectroscopy and X-ray diffraction results demonstrated that a chitosan-alginate hydrogel was constructed due to the strong ionic interaction between the positively charged amino groups of chitosan and the negatively charged carboxyl groups of alginate. The scanning electron microscopy and contact angle results showed the inner porous structure and highly hydrophilic property of chitosan-alginate hydrogel. As the two most promising cell types in nerve tissue engineering, both olfactory ensheathing cells and neural stem cells proliferated well on the chitosan-alginate hydrogel. All results indicated the good potential application of a chitosan-alginate hydrogel for neural tissue engineering.

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Section: Cell Proliferationmentioning

confidence: 99%

Feasibility of chitosan-alginate (Chi-Alg) hydrogel used as scaffold for neural tissue engineering: a pilot studyin vitro

Wang

Huang

2017

Biotechnology & Biotechnological Equipment

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate otologic tissue engineering 15,25‐35,38‐43,45,46,107‐144 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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“…A study by Filardo et al (24) showed improvement in postoperative scaffold stability and integrity using fibrin glue. Wang et al (25), however, reported that a bone matrix gelatin and fibrin glue scaffold showed signs of degradation after 8 weeks despite the presence of supporting chondrocyte attachment, proliferation, and biosynthesis of cartilaginous matrix components. Another study proposed that fibrin sealant supports the migration and proliferation of human chondrocytes via thrombin (26).…”

Section: Amic Microfracturing Osteochondral Defects Osteochondrosis Dmentioning

confidence: 99%

Functional Medium-Term Results After Autologous Matrix-Induced Chondrogenesis for Osteochondral Lesions of the Talus: A 5-Year Prospective Cohort Study

Gottschalk

Altenberger

Baumbach

et al. 2017

The Journal of Foot and Ankle Surgery

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Autologous matrix-induced chondrogenesis (AMIC) has gained popularity in the treatment of osteochondral lesions of the talus. Previous studies have presented promising short-term results for AMIC talar osteochondral lesion repair, a 1-step technique using a collagen type I/III bilayer matrix. The aim of the present study was to investigate the mid-term effects. The 5-year results of a prospective cohort study are presented. All patients underwent an open AMIC procedure for a talar osteochondral lesion. Data analysis included general demographic data, preoperative magnetic resonance imaging findings, intraoperative details, and German version of the Foot Function Index (FFI-D) scores preoperatively and at 1 and 5 years after surgery. The primary outcome variable was the longitudinal effect of the procedure, and the influence of various variables on the outcome was tested. Of 47 consecutive patients, 21 (45%) were included. Of the 21 patients, 8 were female (38%) and 13 were male (62%), with a mean age of 37 ± 15 (range 15 to 62) years and a body mass index of 26 ± 5 (range 20 to 38) kg/m. The defect size was 1.4 ± 0.9 (range 0.2 to 4.0) cm. The FFI-D decreased significantly from preoperatively to 1 year postoperatively (56 ± 18 versus 33 ± 25; p = .003), with a further, nonsignificant decrease between the 1- and 5-year follow-up examination (33 ± 25 versus 24 ± 21; p = .457). Similar results were found for the FFI-D subscales of function and pain. The body mass index and lesion size showed a positive correlation with the preoperative FFI-D overall and subscale scores. These results showed a significant improvement in pain and function after the AMIC procedure, with a significant return to sports by the 5-year follow-up point. The greatest improvement overall was seen within the first year; however, further clinical satisfaction among the patients was noticeable after 5 years.

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Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering

Cited by 10 publications

References 13 publications

Feasibility of chitosan-alginate (Chi-Alg) hydrogel used as scaffold for neural tissue engineering: a pilot studyin vitro

Feasibility of chitosan-alginate (Chi-Alg) hydrogel used as scaffold for neural tissue engineering: a pilot studyin vitro

Tissue engineering applications in otolaryngology—The state of translation

Functional Medium-Term Results After Autologous Matrix-Induced Chondrogenesis for Osteochondral Lesions of the Talus: A 5-Year Prospective Cohort Study

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