Evaluation of bovine (Bos indicus) ovarian potential for in vitro embryo production in the Adamawa plateau (Cameroon)

Kouamo, Justin; Dawaye, SM; Zoli, A; Bah, G. S.

doi:10.5455/ovj.2014.v4.i2.p128

Cited by 16 publications

(15 citation statements)

References 20 publications

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“…Cows between 6 and 9 years old had a higher oocyte maturation rate than cows over 10 years old. This result agrees with that of Natumanya et al (2008) and Kouamo et al (2014), indicating a significantly higher oocyte maturation rate in cows belonging to the 6-9-year age group.…”

Section: Discussionsupporting

confidence: 93%

“…The average number of follicles per cow in this study was 24.71 ± 0.88. This value is higher than the 5.20 reported by Kumar et al (1997) in India and 23 examined by Taneja et al (2000) in the same country but lower than the 37.5 ± 25.2 follicles estimated by Kouamo et al (2014) in Cameroun and 32 follicles measured by Takaji et al (1992) for the ovaries of a cow reared in Japan. This variation in the number of follicles would be linked to parity (number of births) and the breed of the cow (Rhodes et al, 1995).…”

Section: Discussioncontrasting

confidence: 59%

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Prevalence and Antimicrobial Susceptibility of Escherichia coli Isolated from Goats in the Mekong Delta, Vietnam

Hachim¹,

Hervé²,

Jean-Pierre³

et al. 2024

WVJ

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A cow can give birth to an average of 6-7 calves in her entire reproductive period. The remaining oocytes could be used for the in vitro production of embryos. The present study was conducted to evaluate the effects of ovarian and non-ovarian factors on the follicular population and oocyte maturation of three Chadian cattle breeds (Arab, Kouri, and Toupouri). For this purpose, the ovaries of 166 cycled cows were collected at the Farcha slaughterhouse of Chad and placed individually in labeled conical tubes containing 0.9% NaCl and 0.5 mg/ml penicillin-streptomycin. After clearing the ovaries of tissue debris, they were weighed, and the follicles were counted. The diameter of each follicle was measured and classified into three categories. A total of 2734 oocytes were collected in 28 days with a minimum of 97 per day by the slicing method using a 10X stereoscope. They were then classified into four groups according to the structure of their cumulus oophorus. Immature oocytes (class 1 and 2 [1455]) were placed in different culture media consisting of Minimum Essential Medium (MEM) alone, MEM with 10% follicular fluid, and MEM with 50% follicular fluid for oocyte maturation. The results indicated that the mean follicular population and mean oocyte yield were 24.71 ± 0.88 and 11.65 ± 0.94, respectively. The mean oocyte index and the number of cultivable oocytes for in vitro embryo production (class 1 and 2) were 1.03 ± 0.23 and 1.65 ± 0.94, respectively. The number of follicles observed in the age group of 6-9 years was higher than in other age groups. Oocyte yield was significantly higher in cows with a body condition score of 4-5 compared to average and lean cows. Among the different culture media used for oocyte maturation, the medium consisting of MEM plus 10% follicular fluid recorded a higher maturation rate than the other culture media. Cows aged 6-9 years had a higher maturation rate than other age groups. In conclusion, the good follicle (follicle that produced oocyte) and appropriate oocyte performance were observed in cows with body condition score 3-5 and an age range of 6-9 years.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 59%

Prevalence and Antimicrobial Susceptibility of Escherichia coli Isolated from Goats in the Mekong Delta, Vietnam

Hachim¹,

Hervé²,

Jean-Pierre³

et al. 2024

WVJ

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“…The follicles of each ovary were measured by a Vernir and classified according to these measurements into three groups: small with a diameter of less than (3 mm), medium with a diameter of (3-8 mm), and large with a diameter greater than (8 mm). These follicles were counted and recorded for further analysis (Figure 2) according to [14].…”

Section: Classifying Follicles and Measuring Their Dimensionsmentioning

confidence: 99%

Effect of level of Interleukin 1 beta (Il-1B) and Luteinizing hormone (LH) impacts on the size of the ovarian follicles of local cows

Al-Janabi,

Aldulaimi

2023

JKAS

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This study was conducted in the laboratories of the Department of Animal Production Techniques at the Technical College in Mussaib (TCM) from September 2021 to January 2022 to study the relationship between Interleukin 1 beta (il-1B) and Luteinizing hormone (LH) with ovarian follicle size on local cows. 150 reproductive systems were collected from non-pregnant local cows, which slaughtered in the abattoirs of Babylon Province and were transported to the laboratory within two hours. Ovaries were removed (300 ovaries) and their dimensions were measured. The follicles were measured and classified into three categories. Follicular fluid was drawn from the visible follicles (2-8) on the surface of each ovary and placed in an Eppendorf tube to measure the concentration level of Interleukin 1 beta (il-1B) and Luteinizing hormone (LH) in the follicular fluid. The results showed a significant high superiority (P<0.01) of an increase in the level of interleukin-1beta over the size of large, medium and small follicles. A significant high effect (P<0.01) of increasing the level of the ovulation hormone on follicle size was observed for all small, medium and large groups. We conclude from this study that the concentrations of cytokines (interleukin-1beta) and hormones (Luteinizing hormone) are influenced by the size of the ovarian follicle.

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“…The collection of ovaries from slaughterhouses offers a feasible and abundant source of primary oocytes for the large-scale production of embryos through in vitro maturation (IVM) and in vitro fertilization (IVF) (Kouamo et al, 2014). However, the follicular population, oocyte yield, and quality of local cattle breeds are often suboptimal, influenced by various factors including breed, age, weight, body condition score (BCS), and physiological, nutritional, and pathological status of females (Dorice et al, 2019;Hachim et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Ovarian potential of Cameroonian Zebu cattle (Bos indicus) slaughtered in Etoudi – Yaoundé

Katte,

Herve,

Dorice

et al. 2023

J Anim Reprod Biotechnol

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Background: This study helps to evaluate the Ovarian potential of Cameroonian Zebu cattle (Bos indicus) slaughtered in Etoudi -Yaoundé for implementing Assisted Reproductive Techniques (ARTs). The aim was to enhance the productivity of the cattle sub-sector in Cameroon while conserving local genetic resources. Methods: A total of 144 cows, including 94 cycled cows and 50 pregnant cows, were included in the study. Live weights were determined by measuring the thoracic perimeter of each cow using a Rondo measuring tape. Age was determined postmortem through examination of dentition and horns, while the uterus and ovaries were removed, weighed, and classified based on physiological status (pregnant or nonpregnant). Follicles were counted, and their diameters were measured and categorized into small (Ø < 3 mm), medium (Ø 3-8 mm), and large (Ø > 8 mm). Results: The results revealed an average follicular population of 32.02 ± 9.31 per cow, with 22.43 ± 8.45 small follicles, 8.42 ± 3.87 medium follicles, and 0.76 ± 0.34 large follicles. The weight of the right ovary was significantly higher than that of the left ovary (p < 0.05), and cows aged 6 to 9 years exhibited a higher number of follicles compared to other age groups. Cows with medium (BCS = 3) and large (BCS = 4-5) body reserves had the heaviest ovaries. Additionally, pregnant cows had heavier uteri compared to non-pregnant cows, and cows with a body condition score of 3 or higher had higher uterine weights than lean cows (BCS = 1-2). Conclusions: This study demonstrates that age, body condition score, and pregnancy status influence ovarian weight. Body Condition Score serves as a reliable indicator of cow nutritional status, and cows with BCS of 3-5 are excellent candidates for in vitro production of embryos.

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Evaluation of bovine (Bos indicus) ovarian potential for in vitro embryo production in the Adamawa plateau (Cameroon)

Cited by 16 publications

References 20 publications

Prevalence and Antimicrobial Susceptibility of Escherichia coli Isolated from Goats in the Mekong Delta, Vietnam

Prevalence and Antimicrobial Susceptibility of Escherichia coli Isolated from Goats in the Mekong Delta, Vietnam

Effect of level of Interleukin 1 beta (Il-1B) and Luteinizing hormone (LH) impacts on the size of the ovarian follicles of local cows

Ovarian potential of Cameroonian Zebu cattle (Bos indicus) slaughtered in Etoudi – Yaoundé

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