Evaluation of BPA effects on autophagy in Neuro-2a cells

Li, Jinglong; Yin, Zhihong; Hua, Liushuai; Wang, Xinrui; Ren, Fei; Ge, Yaming

doi:10.1177/07482337221076587

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…These associations suggest that decreased autophagy in the HF + BP group may be linked to changes in the eIF2α/ATF4 pathway [13]. In a previous study using Neuro-2a cells, autophagy was decreased by BPA, as determined by the reduction in beclin-1 and LC3-II/LC3-I levels, suggesting BPA-induced neurotoxicity [38]. A macrophage study also showed a BPA-induced reduction in LC3-II/LC3-I levels and an increase in p62 levels, contributing to BPA-induced immune cell injury [39].…”

Section: Discussionmentioning

confidence: 78%

Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice

Kim,

Youn

et al. 2023

Foods

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The simultaneous exposure to a high-fat (HF) diet and to bisphenol A (BPA) from delivered foods and food-delivery containers is on the rise in humans, according to the increased frequency of food delivery during the COVID-19 pandemic. This co-exposure could cause harmful tissue toxicity in the human body. Here, the preventive effect of Allium macrostemon Bunge (AM) extract against dysfunction in adipose tissue and the liver under co-exposure to BPA and an HF diet was examined using mice. C57BL/6N mice were divided into four groups (n = 6 or 7/group) according to diet and treatment: control diet with vehicle (CON), HF diet with vehicle (HF), HF diet with an oral injection of BPA (HF + BP), and HF diet with an oral injection of BPA and AM extract (HF + BP + AM). HF feeding increased body weight gain compared to CON feeding, while BP + HF and BP + HF + AM feeding suppressed body weight gain compared with HF feeding. The BP + HF group had lower body weight than the HF group, but the two groups had similar epididymal fat mass. The HF + BP + AM group showed lower pro-inflammatory gene expression levels in adipose tissue and epididymal fat mass compared to the HF + BP group. Altered endoplasmic reticulum (ER) stress response in the liver was partly observed in the HF + BP group, as shown by increased total phosphorylated Jun N-terminal kinase protein levels compared to those in the HF group. In addition, ecdysterone 25-O-β-D-glucopyranoside and 6-gingerol were identified in AM extract by mass spectrometry and molecular networking analysis. In summary, the AM extract diminished adipose tissue inflammation and hepatic ER stress in an HF diet and BPA co-exposure condition. To utilize AM as a potential food component to alleviate the harmful effect of an HF diet and BPA exposure, further research investigating the specific impact of AM extract supplementation using additional experimental groups or various treatment doses is warranted.

show abstract

Section: Discussionmentioning

confidence: 78%

Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice

Kim,

Youn

et al. 2023

Foods

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show abstract

“…In a previous study using Neuro-2a cells, autophagy was decreased by BPA, as determined by the reduction in beclin-1 and LC3-II/LC3-I levels, suggesting BPA-induced neurotoxicity (Li et al 2022). A macrophage study also showed a BPA-induced reduction in LC3-II/LC3-I levels and an increase in p62 levels, contributing to BPA-induced immune cell injury (Wu et al 2022).…”

Section: Discussionmentioning

confidence: 90%

Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice

Kim

Youn

Seo

et al. 2023

Preprint

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Exposure of humans to high-fat (HF) diet and bisphenol A (BPA) has increased in recent times, and co-exposure to these causes harmful tissue toxicity. Here, the preventive effect of Allium macrostemon (AM) extract against dysfunction of adipose tissue and the liver under co-exposure to BPA and HF diet was examined. C57BL/6 mice were fed control (CON) diet, HF diet, HF diet with an oral injection of BPA (HF + BP), or HF diet with an oral co-injection of BPA and AM extract (HF + BP + AM). While HF feeding increased body weight gain compared to CON feeding, BP + HF suppressed body weight gain. BP + HF with lower body weight than HF had similar epididymal fat mass and inflammatory stress as HF, but significantly higher serum triglyceride and free fatty acid levels. In the liver, altered endoplasmic reticulum (ER) stress response and decreased autophagy were observed in HF + BP, as shown by increased t-IRE1α, p-JNK, and p62 levels and decreased beclin-1 protein levels, compared to those in HF. HF + BP + AM reduced HF and BPA-induced pro-inflammatory responses in adipose tissue, ER stress in liver, and total cholesterol level in serum. In addition, ecdysterone 25-O-β-D-glucopyranoside and 6-gingerol were firstly identified in AM extract by mass spectrometry and molecular networking analysis. In summary, the AM extract diminished liver and adipose tissue dysregulation, including hepatic ER stress and adipose tissue inflammation while a HF diet and BPA co-exposure induced harmful health outcomes in adipocytes and the liver. Therefore, AM has the potential to alleviate the harmful effects of a HF diet and BPA via anti-ER stress and anti-inflammatory activities.

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Bisphenol A-induced autophagy ameliorates human B cell death through Nrf2-mediated regulation of Atg7 and Beclin1 expression by Syk activation

Park

Jang

Moon

2023

Ecotoxicology and Environmental Safety

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Evaluation of BPA effects on autophagy in Neuro-2a cells

Cited by 4 publications

References 38 publications

Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice

Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice

Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice

Bisphenol A-induced autophagy ameliorates human B cell death through Nrf2-mediated regulation of Atg7 and Beclin1 expression by Syk activation

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