Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum

Abstract

Fifty clinical isolates ofwere included as quality controls. All isolates produced clearly detectable growth only after 7 days of incubation. MICs were significantly independent of the incubation temperature (28 or 35°C) (P < 0.05). Different incubation periods resulted in MICs which were consistently different for each medium when azoles and griseofulvin were tested (P < 0.05). MICs obtained from different media at the same incubation time for the same isolate were significantly different when azoles and griseofulvin were tested (P < 0.05). MICs were consistently higher (usually 1 to 2 dilutions) with RPMI than with MVM or SDB (P < 0.05). When terbinafine was tested, no parameter had any influence on MICs (P < 0.05). RPMI standard medium appears to be a suitable testing medium for determining the MICs for T. rubrum. MICs obtained at different incubation times need to be correlated with clinical outcome to demonstrate which time has better reliability.

In vitro susceptibility testing of dermatophytes isolated from pediatric cases in Nigeria against five antifungals

“…Nevertheless, our result on terbinafine as the most active agent for example, agrees with the observation from previous authors in other continents and region 5,18 . This antimycotic showed an excellent in vitro potency and broad-spectrum activity against all the tested species.…”

“…We therefore recorded our MIC values after four days of incubation. However, our results disagreed from SANTOS & HAMADA 18 and from FERNANDEZ-TORRES et al 5 . In another study FERNANDEZ-TORRES et al 4 tested 508 strains belonging to 24 species of dermatophytes against conventional (itraconazole and fluconazole) and some newer antifungal agents like voriconazole and UR-9825, with similar results.…”

<strong>4,6,6-trimethylbicyclo [3.1.1] hept-3-ene: Analysis of the Inhibitory Effect of Monoterpere on <em>Pseudomonas aeruginosa</em> Strain</strong>

“…The experiments were performed in triplicate and the result was expressed by the arithmetic mean of the MICs obtained in the three trials [11].…”

Medium Optimization for Production of Beauveriabassiana BNBCRC Spores from Biohydrogen Effluent of Palm Oil Mill Using Taguchi Design

“…Sterile distilled water (10 mL) was added on the fungal colonies. The spore suspensions were prepared by scraping using a spreader and gently probing the surface with the tip of a Pasture pipette [12]. The spore suspension was mixed thoroughly using a vortex and diluted to obtain a suspension of 1×10 6 spores mL -1 .…”