Evaluation of Brucellacapt for the diagnosis of human brucellosis

Casao, María Angeles; Navarro, Elena; Solera, J

doi:10.1016/j.jinf.2004.03.004

Cited by 34 publications

(30 citation statements)

References 14 publications

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“…Brucellacapt ve Coombs testinin insan brusellozunun tanısında benzer duyarlılık ve özgüllük gösterdiğini söylemek mümkündür. Buna ek olarak brucellacapt, Coombs testinde olduğu gibi, SAT ile tespit edilemeyen değişim geçirmiş uzun süreli olgularda tanıya yardımcı olabilmektedir [9].…”

Section: Discussionunclassified

Nonspesifik semptomlu olgularda bruselloz sıklığı

Yıldırım¹,

Sümer²

2013

CMJ

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ÖzetAmaç. Brucella spp. türü bakterilerin neden olduğu Bruselloz, tüm dünyada olduğu gibi ülkemizde de endemik olarak bulunmaktadır. Bu çalışmada 2011 Haziran, 2012 Ocak ayları arasında ateş, eklem ağrısı, halsizlik ve yorgunluk şikayeti ile çeşitli servislerden laboratuvarımıza başvuran 1429 hastada, brucellacapt testi sonuçları ve sedimentasyon sonuçlarının retrospektif olarak incelenmesi amaçlanmıştır. Yöntem. Testte Coombs test antijeni (Metser, İSTANBUL) kullanılarak brucellacapt yöntemi uygulandı. Sedimentasyon (Vacoplus, ESR120) değerlerine autoanalyzer cihazında fotometrik tüp yöntemi ile bakıldı. Bulgular. Çalışmamızda toplamda 103 hastada %7,2 lik bir pozitiflik oranı görüldü. Pozitif hastaların %6,2'si (n=49) kadın, %8,5'i (n=54) erkekti. Brusella pozitif ve brusella negatif hastalar arasında sedimentasyon değeri açısından doğrudan bir ilişki bulunmadı (p≥0,195). Sonuç. Morbiditesi yüksek bir hastalık olan bruselloz, bölgemizde sık denilecek bir oranda görülmektedir. Nonspesifik semptomları olan hastalarda, akılda tutulması ve araştırılması gerekmektedir.Anahtar sözcükler: Brucella spp., brucelloz, brucellacapt testi, sedimentasyon Abstract Aim. Brucellosis, caused by Brucella spp. type bacterium is an endemic disease in our country like all over the world. In this study, we aimed analysing retrospectively the results of brucellacapt test and sedimantation in 1429 patient applicated to our laboratory with high temperature, joint pain, weakness and fatigue complaints between 2011 June and 2012 January from different services. Method. In the test, brucellacapt method was performed using Coombs test antigen. Sedimantations were evaluated with photometric tube method in autoanalyzer device. As a total, 7.2% positivity was detected in 103 patient 6.2% of patients detected positive were women (n=49) and, 8.5% of them are male (n=54). Result. There is no correlation for sedimantation between brucella positive and brucella negative patients (p≥0,195). Conlusion. Brucellosis disease that has high morbidity occurs in our region frequently. It must be thought and researched in the patients who have nonspecific symptoms. GirişBruselloz, Brucella türü bakterilerin neden olduğu, tüm dünyada yaygın görülen bir zoonozdur. Bruselloz çiftlik hayvanlarından insanlara bulaşır, çoklu sistem tutulumu ile seyreder. Brucella spp. vücutta birçok sistemi tutabilmektedir, enfeksiyon sırasında özellikle lenf nodlarına yerleşir, bakteriyemi oluşturur [1].

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Section: Discussionunclassified

Nonspesifik semptomlu olgularda bruselloz sıklığı

Yıldırım¹,

Sümer²

2013

CMJ

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“…The Brucellacapt test has recently been developed by the Vircell Company (Santa Fe, Granada, Spain) as an immunocapture-agglutination test; it uses dilution buffer at pH 5.0, a cell suspension of Brucella in buffer, also at pH 5.0, and U-bottomed microtiter plates coated with anti-total human immunoglobulin, included in the Brucellacapt kit. Reported results have been very promising and are closely related to those of the Coombs test; however, it has been suggested that they may become a better marker of disease activity (4,16).…”

Section: Discussionmentioning

confidence: 99%

Secondary Serological Response of Patients with Chronic Hepatosplenic Suppurative Brucellosis

Díaz

Ariza

Alberola

et al. 2006

Clin Vaccine Immunol

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Chronic hepatosplenic suppurative brucellosis (CHSB) is a local reactivation of a previous brucellosis, coursing with an immunoglobulin G (IgG) and IgA secondary immunological response. The observation of two cases of CHSB with an apparent IgM response gave rise to a detailed serological study of three of our patients. We studied the first sample from all three patients and successive samples from two of them. In cases 1 and 2, we found samples with positive IgM lateral flow and IgM enzyme-linked immunosorbent assay results concomitantly with rheumatoid factor (RF); after absorption with anti-RF serum, these results were rendered negative. In patients 2 and 3 the diagnosis of brucellosis was delayed, because none of the test results were initially very significant. However, a clear seroconversion of IgG antibodies was observed in subsequent months; titers of the Brucellacapt and Coombs tests increased in similar ways, although Brucellacapt decreased more rapidly than Coombs, which persisted at high titers for years. In patient 3 a relapse was observed in the fourth year of follow-up, detected by Coombs and also by IgG lateral flow and counterimmunoelectrophoresis (CIEP), although not by the rose bengal, agglutination, or Brucellacapt tests. Serological changes in CHSB may sometimes be mild and are detected mainly by the Coombs test. Brucellacapt does not offer additional information, although IgG lateral flow and CIEP may be of some use. Careful surveillance of titer changes in the Coombs test is the best marker of infection activity. As the disease progresses, an intense IgG response may develop and RF sometimes appears, simulating an IgM response.

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“…However, they vary considerably regarding several aspects including: applications in various situations and usefulness as reflected their sensitivity and specificity as shown in Table 2, [49,63,188,[217][218][219][220][221][222][223][224][225][226][227][228][229][230].…”

Section: Serology In the Diagnosis Of Brucellosismentioning

confidence: 99%