Evaluation of Brugia malayi sheath protein (Shp-1) as a diagnostic antigen for human lymphatic filariasis

Jawaharlal, Jeya Prita Parasurama; Ravishankaran, Rajendran; Shridharan, Radhika Nagamangalam; Lawrence, Ansel Vishal; Karande, Anjali A.; Kaliraj, Perumal

doi:10.1016/j.diagmicrobio.2013.11.019

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…malayi were positive with the assay. Furthermore, when compared with two other ELISA sandwich assays, WbSXP-1 and Og4C3, only the anti-BmShp-1 ELISA gave positive results for healthy individuals from the endemic area (12%) and those with chronic pathology (29%), highlighting the potential use for this assay in monitoring the effectiveness of mass drug administration [ 84 ].…”

Section: Diagnostic Testsmentioning

confidence: 99%

Recombinant antigens used as diagnostic tools for lymphatic filariasis

et al. 2021

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Lymphatic filariasis (LF) is a parasitic disease caused by the worms Wuchereria bancrofti, Brugia malayi, or Brugia timori. It is a tropical and subtropical illness that affects approximately 67 million people worldwide and that still requires better diagnostic tools to prevent its spread and enhance the effectiveness of control procedures. Traditional parasitological tests and diagnostic methods based on whole protein extracts from different worms are known for problems related to sample time collection, sensitivity, and specificity. More recently, new diagnostic tools based on immunological methods using recombinant antigens have been developed. The current review describes the several recombinant antigens used as tools for lymphatic filariasis diagnosis in antigen and antibody capture assays, highlighting their advantages and limitations as well as the main commercial tests developed based on them. The literature chronology is from 1991 to 2021. First, it describes the historical background related to the identification of relevant antigens and the generation of the recombinant polypeptides used for the LF diagnosis, also detailing features specific to each antigen. The subsequent section then discusses the use of those proteins to develop antigen and antibody capture tests to detect LF. So far, studies focusing on antibody capture assays are based on 13 different antigens with at least six commercially available tests, with five proteins further used for the development of antigen capture tests. Five antigens explored in this paper belong to the SXP/RAL-2 family (BmSXP, Bm14, WbSXP-1, Wb14, WbL), and the others are BmShp-1, Bm33, BmR1, BmVAH, WbVAH, BmALT-1, BmALT-2, and Wb123. It is expected that advances in research with these antigens will allow further development of tests combining both sensitivity and specificity with low costs, assisting the Global Program to Eliminate Lymphatic Filariasis (GPELF).

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Section: Diagnostic Testsmentioning

confidence: 99%

Recombinant antigens used as diagnostic tools for lymphatic filariasis

et al. 2021

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“…The results were articulated as mean absorbance at 450 nm for each group ± standard deviation (OD±SD). Samples were identified as positive when the optical density was higher than the mean OD+3SD value of 10 NEN control sera [11] .…”

Section: Methodsmentioning

confidence: 99%

“…The ICT filarial antigen test and the Og4C3 assay are based on detection of adult worm antigens [9] , [10] . Later, detection assays utilizing the mf stage antigens were developed using various targets such as r Wb Shp-1, Bm 14 and r Wb SXP-1 [11] , [12] , [13] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Rapid Blood Sample Collection in the Detection of Circulating Filarial Antigens for Epidemiological Survey by rWbSXP-1 Capture Assay

et al. 2014

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BackgroundLymphatic filariasis is a neglected tropical disease leading to profound disfiguring causing socio economic burden in the tropics. Current diagnosis strategies available during field surveys and epidemics are based on traditional microscopic detections and a few antigen/antibody assays. We have compared different sampling methodologies and standardized the highly sensitive and reliable rWbSXP-1 antigen detection assay to our new sampling methodology.MethodologySamples collected as serum, whole blood, whole blood on filter paper and whole blood on microscopic slides from patients belonging to various clinical groups of filariasis [endemic normal(EN), chronic pathology(CP), microfilaraemic(MF) and non-endemic normal(NEN)] were collected and standardized the rWbSXP-1 antigen detection assay using monoclonal antibody raised against rWbSXP-1 protein. The whole blood collected on microscopic slide based sampling method was employed in the field and the presence of circulating filarial antigen (CFA) was assessed using the rWbSXP-1 assay.Principal FindingsThe sampling methods were compared and no significant difference was observed for the detection of CFA (MF, P = 0.304, EN, P = 0.675, CP, P = 0.5698, NEN, P = 0.4494). Further the optimized sampling method was utilized to collect the 1106 samples from Polur, Tiruvannamalai. The rWbSXP-1 assay gave 98 antigen positive results whereas the microscopic method gave only 17.ConclusionsFour sampling methodologies were analyzed and the new sampling methodology of whole blood collected on microscopic slide was found to be convenient for the detection of CFA using rWbSXP-1 antigen detection assay. The 1106 samples from Polur were collected using the new method. The rWbSXP-1 antigen assay perceived a 7.32% increased result which was read as false negatives on the conventional microscopic staining method. This new sampling methodology coupled with the rWbSXP-1 antigen assay can be used in epidemiological surveys for lymphatic filariasis and the same sampling methodology can be expanded to other antigen based high affinity assays.

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“…For expression of Shp-1, E. coli GJ1158 strain was transformed with the plasmid pRSET-B Shp-1 and cultures at OD of 0.6 were induced with 100 mM NaCl for 3 h. Shp-1 was purified by electro-elution method as described previously by Jawaharlal et al [8]. The purified protein was passed through polymyxin B affinity column to remove endotoxin and analyzed by Limulus Amebocyte Lysate (LAL) test using chromogenic endotoxin quantitation kit (Thermo Fisher Scientific, Rockford, IL).…”

Section: Recombinant Production Of Rbmshp-1 In E Colimentioning

confidence: 99%