Evaluation of by-products from the biodiesel industry as fermentation feedstock for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Cupriavidus necator

Impact of different by-products from the biodiesel industry and bacterial strains on the production, composition, and properties of novel polyhydroxyalkanoates containing achiral building blocks

“…4 shows thermogravimetric curves and associated derivatives. Thermal degradation occurred in one or two stages of weight loss, which ranged between 95.29% (B. cepacia IPT 438 with CG III) and 98.96% (C. necator IPT 027 with CG II) and indicated the minimal presence of remaining impurities from the process of PHA extraction (González- García et al, 2013). The degradation mechanisms were similar to the mechanisms discussed by Zhu et al (2010) and Campos et al (2014).…”

Strategies for Large-scale Production of Polyhydroxyalkanoates

“…[40][41][42][43][44][45][46][47][48][49][50] Production of mcl-PHAs and other interesting PHA copolymers including scl-copolymers and scl-mcl copolymers has also been reported using unconventional substrates, such as lignocellulosics e.g., grass mass hydrolysate, rice bran, rapeseed meal hydrolysate, and industrial waste e.g., whey, biodiesel waste, vinnase etc. 35,48,[51][52][53][54][55][56] Utilization of waste whey for PHA production is particularly useful as only 50 % of the whey produced is used for production of food ingredients and animal feed, while the rest remains as a pollutant owing to its high biological oxygen demand. Production of scl-mcl copolymer using whey adds additional merit, as such copolymers are natural blends that combine the interesting chemical, mechanical and thermal properties of both scl and mcl PHAs.…”

“…73 Fed-batch cultivation using crude glycerol and rapeseed meal hydrolysate by C. necator DSM 545 demonstrated a two-fold increase in production of P(3HB-co-3HV) copolymer to 10.9 g L -1 as compared to a polymer concentration of 5.5 g L -1 obtained in batch cultivation. 54 Production of a PHA copolymer comprising predominantly 4 mcl-PHA monomer units was reported using an isolated thermotolerant Pseudomonas SG4502 strain when grown on biodiesel waste. A biomass concentration of 1.5 g L -1 and PHA content of 40.6 % dcw was obtained by fed-batch cultivation.…”

Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source

“…For example, PHB co-polyesters containing 3-hydroxyvalerate (P3HB-co-3HV) have reduced crystallinity between 33% and 49%. (Lee et al, 2008;Han et al, 2010;García et al, 2013). The P(3HB) produced by the recombinant E. coli using crude glycerol as the sole carbon source showed a significantly low crystallinity that was comparable to that of P3HB-co-3HV (Lee et al, 2008).…”