Evaluation of C. pareira L stem and T. peruviana (pers.) K. Schum leaf for estrogenic activity

Abstract

Context: Methanolic extract of C. pareira stem and T. peruviana leaves are capable of producing antifertility activity in female rat by increasing estrogen level and decreasing progesterone level. Aim: The aim of the present study is to C. pareira L stem and T. peruviana (Pers.) K. Schum leaf for estrogenic activity. Material and Method: Methanolic extract were made from C. pareira stem and T. peruviana leaves (after removal of cardiac glycoside) using cold maceration process. Extracts were tested for estrogenic activity on female immature rat (50-60 gm) at dose level of 250mg/kg. Obtained data were compared with control and standard (estrogen treated) group data. Statistical Analysis: Statistical analysis of all data was made with statistical packages (GraphPad Instat) and ANOVA followed by Dunnett’s ttest was used for statistical analysis. Result: Decrease in the absolute weight of the uterus and uterine weight to body weight ratio in CPS-Me and TPL-Me-G group treated were significant (p<0.05) as compared to control. Conclusion: In our earlier studies it was found that methanolic extract of C. pareira stem and T. peruviana leaves increased serum estrogen level and decreased progesterone level in pregnant rat. In the present study, these extracts decreased the absolute weight of the uterus and uterine weight to body weight ratio significantly (p<0.05). So, we can conclude that these two-extract possessed partial estrogenic activity.

Cissampelos pareira ethanolic extract modulates hormonal indices, lipid profile, and oxidative parameters in transient infertility-induced female albino rats

“…The leaves and aerial parts have been used for birth control and to induce abortion in human (Tiwari et al, 1982;Dangol and Gurung, 1991). The root has also been reported to be used traditional for birth control and as anti-fertility agent (Maurya et al, 2004;Samanta et al, 2015). Similarly, in Africa, reports on the fertility-related traditional usage of C. pareira are contrasting.…”

“…Moreover, several pharmacological studies have demonstrated the crude extracts of C. pareira to possess various activities such as antipyretic, anti-inflammatory, antiarthritic, antiulcer, antioxidant, antidiabetic, anticancer, antimicrobial, antioxidant, antivenom, antifertility, antimalarial and immunomodulatory (Kumari et al, 2021). Till date, despite the popular traditional usage of C. pareira in the management of fertility related conditions, scientific study of this plant to this effect remains fragmentary, as a few, findings from scientific studies indicated the antifertility properties of C. pareira (Samanta et al, 2015;Ganguly et al, 2007), the traditional usage and claim of Cissampelos spp. as a pro-fertility agent persists, hence, the need for more scientific investigation for verification of these claims and usages.…”