Evaluation of C-Reactive Protein in Iraqi Children Presented with Acute Enteropathogenic Escherichia Coli Associated Diarrhea with Special Emphasis to Age and Gender

Abstract

Background and Objectives:The aim is to determine the role of Enteropathogenic Escherichia coli (EPEC) serotypes (O111, O55, O26, O86, O119, O127, O114, and O142) in C-reactive protein (CRP) level in acute diarrhea among children; to determine the possible effect of age and gender in CRP level. Methods: Culture of stool samples from 64 children in MacConkey agar for primary isolation of E.coli and confirmed via API20E . Sero-grouping of E.coli performed via specific antisera . CRP detected by latex agglutination. Results: In this study, 64 children with diarrhea were involved. The mean age was 2.92 years. Males represent 34/64, 53.1%. The range of CRP level 12-96 mg/dl. EPEC1 and EPEC3 not detected. EPEC2 detected among 14/64, 21.9% and EPEC4 in 18.8%. Among the age group ˂1 year, EPEC2 detected in 9.38%, with mean CRP level 12mg/dl; 3.13%, 48 mg/dl 6.25% . EPEC4 detected in 2/64, 3.13%, with mean CRP level 12mg/dl. Age group 1-4 year, EPEC2 detected in 6/64 , 9.38% , with mean CRP level 24mg/dl. EPEC4 detected in 6/64, 9.38%; with mean CRP level 12mg/dl, 24mg/dl, 48mg/dl 3.13% in each. Among the age group 4.1-8 year, EPEC4 detected in 4/64 ,6.25% , with CRP mean level 48 mg/dl. EPEC not detected in 8˃ years age group .EPEC2 detected in male 28/64 , 43.75% and the CRP level range from 12-96mg/dl .EPEC2 detected in 22/64 females, 34.38% , CRP level range from 12-96mg/dl . No significant difference or correlation between gender and CRP level detected. EPEC4 detected in males 26/64 , 40.63% , CRP level range from 12-96mg/dl . EPEC4 detected in 26/64 females , 40.63% , CRP level range from 12-96mg/dl . No significant difference or correlation between gender and CRP level was detected. EPEC2 and CRP level significantly correlated with patients age . while infection with EPEC2 inversely correlated EPEC4. No significant correlation between gender and EPEC serotypes. Conclusion: Although EPEC2 and EPEC4 frequently detected in diarrhea cases of 1-8 years old, only EPEC2 positively correlated with age. Even with a fluctuation in CRP level, it was positively correlated with age and not correlated with EPEC serotypes and gender.

Evaluation of Antioxidant, Nephroprotective and Immunomodulatory Activity of Vitamins C and E -Sodium Selenite in Mice Intoxicated with Sodium Nitrate

“…Data were expressed as (mean±SD) Al-Ezzy et al, 2020). ANOVA test of Vassar Stats online program was used for analysis Hameed and Al-Ezzy, 2019).…”

Effectiveness of Aqueous Versus Alcoholic Extracts of Melia azedarach in Amelioration of Lipid Profile, Liver Enzymes and Innate Inflammatory Indices for White New Zealand Rabbits

“…Blood samples were taken at (0, 7, 14) day for separation of serum as described by Al-Ezzy et al, 2020). Serum cholesterol and triglyceride, total serum protein, albumin, globulin, total serum cortisol, total serum bilirubin, liver enzymes (ALT, AST, Alkaline phosphatase) were determined by Cobas Integra 400 Plus according to (Al-Ezzy et al, 2016;Hameed et al, 2020).…”