Evaluation of Caesalpinia bonducella flower extract for anti-inflammatory action in rats and its high performance thin layer chromatography chemical fingerprinting

Abstract

Objective:The study is aimed to evaluate anti-inflammatory activity of Caesalpinia bonducella Fleming (Caesalpiniaceae) flower extract (CBFE) and to study its effect on radiographic outcome in adjuvant induced arthritis and authentication by high performance thin layer chromatography (HPTLC) chemical fingerprinting.Materials and Methods:CBFE was administered orally (30, 100, and 300 mg/kg b.wt.) and tested for its anti-inflammatory activity in carrageenan-induced inflammation, cotton pellet induced chronic granulomatous inflammation and autacoids-induced inflammation. Effect on radiographic outcome was tested in adjuvant-induced arthritis. CBFE was HPTLC fingerprinted in suitable solvent system.Result:In carrageenan-induced inflammation, CBFE produced significant inhibition in edema volume at all the doses (30, 100 and 300 mg/kg b.wt.) and percentage of inhibition was 28.68, 31.00, and 22.48, respectively as compared to control at 5 h of its administration. In cotton pellet granuloma assay, CBFE significantly decreased the granuloma weight at 300 mg/kg dose level by 22.53%. CBFE (300 mg/kg) caused significant inhibition by 37.5, 44.44, and 35.29% edema volume, at ½, 1 and 3 h after 5-hydroxytryptamine injection, respectively. Radiographic score of animals treated with 300 mg/kg CBFE was significantly decreased when compared to arthritic control animals.Conclusion:The extract was found to possess significant anti-inflammatory activity. CBFE treatment improved the bony architecture in adjuvant-induced arthritis in rats. The developed HPTLC fingerprint would be helpful in the authentication of C. bonducella flower extract.

Phytochemical screening and GC-MS analysis of bioactive compounds in Caesalpinia bonduc L. from Alagarkovil Reserve Forest (ARF), Dindigul District, South India

“…The herb endowed with antidiarrheal property helps to cure diarrhea/ loose motion 4 . As a botanical it is used to treat joint pain and mitigate arthritis 5 . Traditionally the herb is used to cure many skin problems including leucoderma, leprosy, blisters, boils, and wounds 6 .…”

Phytochemical and Network Pharmacology Based Evaluation of Antiepileptic Potential of Identified Metabolites in Argimone mexicana

“…Automation of sample application, detection (spraying or dipping), closed developing chambers providing standardized conditions (relative humidity, saturation, drying), and digital documentation systems for capturing chromatograms are the essential tools in HPTLC. [8,9] Because of easy handling and great progress in instrumentation leading to the reproducibility of data, HPTLC will remain the most important technique for qualitative analysis of plants. With its extreme flexibility concerning choices of mobile phases and detection methods, the technique can cope with the diversity of substances from primary and secondary metabolism.…”