Evaluation of calcium hydrogen carbonate mesoscopic crystals as a disinfectant for influenza A viruses

Nakashima, Ryuji; Kawamoto, Masaomi; Miyazaki, Shigeru; Onishi, Rumiko; Furusaki, Koichi; Osaki, Maho; Kirisawa, Rikio; Sakudo, Akikazu; Onodera, Takashi

doi:10.1292/jvms.16-0603

Cited by 19 publications

(51 citation statements)

References 15 publications

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“…Recently, we generated a meso-structure comprising a fine particle structure of about 50~500 nm, primarily composed of carbon and calcium from mineral components derived from plants and soil. 9 An electrically charged disinfectant (CAC-717) was then made by placing this meso-structure in water. 9 CAC-717 has been demonstrated to inactivate enveloped viruses such as influenza viruses.…”

Section: Introductionmentioning

confidence: 99%

“…9 An electrically charged disinfectant (CAC-717) was then made by placing this meso-structure in water. 9 CAC-717 has been demonstrated to inactivate enveloped viruses such as influenza viruses. 9 Although free alkaline ions are present in CAC-717, upon contact with human and animal tissue the pH is almost physiological (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…9 CAC-717 has been demonstrated to inactivate enveloped viruses such as influenza viruses. 9 Although free alkaline ions are present in CAC-717, upon contact with human and animal tissue the pH is almost physiological (i.e. neutral pH).…”

Section: Introductionmentioning

confidence: 99%

“…neutral pH). 9 Consequently, CAC-717 does not cause irritation in animal eyes. 9 Therefore, by comparison with current chemical disinfectants, such as chlorine bleach, CAC-717 could be a safer alternative.…”

Section: Introductionmentioning

confidence: 99%

“…9 Consequently, CAC-717 does not cause irritation in animal eyes. 9 Therefore, by comparison with current chemical disinfectants, such as chlorine bleach, CAC-717 could be a safer alternative. Due to its potential effectiveness, CAC-717 could be useful in reducing the risk of food poisoning in the food and dairy industries.…”

Section: Introductionmentioning

confidence: 99%

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<p>Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome</p>

Sakudo

Yamashiro

Haritani

et al. 2020

IJN

Self Cite

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Introduction: A previous study demonstrated the virucidal effect of an electrically charged disinfectant (CAC-717), which contains meso-structure nanoparticles, on enveloped viruses (influenza viruses). However, the effect of CAC-717 on other microorganisms and the mechanisms by which CAC-717 inactivates the microorganisms remain unclear. In this study, CAC-717 was further evaluated in terms of its biocidal and virucidal activity as well as its effect on bacterial and viral nucleic acids. Methods: The inactivation effects of CAC-717 against various microorganisms [non-enveloped virus, feline calicivirus (FCV); bacteria, Salmonella enterica and Escherichia coli] were investigated by comparing the viral titer of the medium tissue culture infectious dose (TCID 50) and the D value (estimated treatment time required to reduce the number of microorganisms by 90%). Furthermore, the effects of CAC-717 on viral and bacterial genomic RNA/DNA were examined using a polymerase chain reaction (PCR). Results: Treatment of an equal volume of CAC-717 with cell lysate infected with a nonenveloped virus, feline calicivirus (FCV), reduced the TCID 50. Viral titer dropped below the detection limit after 2 min of treatment. The D value of FCV was 0.256 min (average of multiple endpoint D values) and endpoint D value was 0.341 min. The D value for E. coli and S. enterica was 0.290 min and 0.080 min (average of multiple endpoint D values), respectively and the endpoint D value was 0.545 min and 0.054 min, respectively. In addition, PCR showed the inhibition of nucleic acid amplification of the RNA and DNA genome of FCV and bacteria, respectively. Conclusion: Our findings suggest that CAC-717 inactivates viruses and bacteria by modifying the viral and bacterial nucleic acids.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<p>Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome</p>

Sakudo

Yamashiro

Haritani

et al. 2020

IJN

Self Cite

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Recent advancement in nanomaterial-encapsulated drug delivery vehicles for combating cancer, COVID-19, and HIV-like chronic diseases

Paul

Mukherjee²,

Banerjee

2023

Mater. Adv.

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Inactivation of human norovirus and its surrogate by the disinfectant consisting of calcium hydrogen carbonate mesoscopic crystals

Shimakura

Gen-Nagata

Haritani

et al. 2019

FEMS Microbiology Letters

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Human norovirus is one of the major causes of foodborne gastroenteritis, and it can be easily transmitted from infected person, virus-contaminated foods and environmental surfaces. Effective disinfection method is needed to stop the transmission of human norovirus. CAC-717 is a new disinfectant consisting of calcium hydrogen carbonate mesoscopic crystals. We aimed to evaluate the efficacy of CAC-717 against human norovirus. This study used human norovirus derived from fecal specimens and cultured murine norovirus, which is one of the surrogate viruses for human norovirus. The disinfection effect against murine norovirus was estimated by infectivity assay and transmission electron microscopy. The inactivation effect against human norovirus was assessed by reverse transcription polymerase chain reaction. Disinfection effect of CAC-717 against the infectivity of murine norovirus was shown within 100 s after the CAC-717 treatment, presenting the destruction of viral capsids. The treatment of CAC-717 significantly reduced human norovirus genomic RNA (3.25-log reduction) by the presence of the mesoscopic structure of calcium hydrogen carbonate. CAC-717 stably inactivated human norovirus in stool suspensions. The inactivation effect of CAC-717 against human norovirus was less susceptible to organic substances than sodium hypochlorite. CAC-717 would be a useful alternative for disinfecting human norovirus in contaminated environmental surfaces.

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Evaluation of calcium hydrogen carbonate mesoscopic crystals as a disinfectant for influenza A viruses

Cited by 19 publications

References 15 publications

<p>Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome</p>

<p>Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome</p>

Recent advancement in nanomaterial-encapsulated drug delivery vehicles for combating cancer, COVID-19, and HIV-like chronic diseases

Inactivation of human norovirus and its surrogate by the disinfectant consisting of calcium hydrogen carbonate mesoscopic crystals

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