Evaluation of calcium ion release in calcium hydroxide prototype as intracanal medicament

Sidiqa, Atia Nurul; Zakaria, Myrna Nurlatifah; Artilia, Ira; Dewi, Zwista Yulia; Cahyanto, Arief

doi:10.15562/jdmfs.v0i0.1017

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…Fourier Transform Infrared Spectra of the endodontic sealers used in this study give all the necessary information about chemical structure of the chemical reagents used in this study. The narrow absorption band at 3642 cm -1 is due to covalent bonds and stretching vibrations of the O-H group as reported in the literature [ 40 ]. In this study, for group E-1, at 3385 cm -1 , -OH of phenols is observed.…”

Section: Discussionmentioning

confidence: 79%

Biochemical properties of novel Carbon nanodot-stabilized silver nanoparticles enriched calcium hydroxide endodontic sealer

Ali,

Gul,

Fareed

et al. 2024

PLoS ONE

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Calcium Hydroxide-based endodontic sealer loaded with antimicrobial agents have been commonly employed in conventional root canal treatment. These sealers are not effective against E. faecalis due to the persistent nature of this bacterium and its ability to evade the antibacterial action of calcium hydroxide. Therefore, endodontic sealer containing Carbon nanodots stabilized silver nanoparticles (CD-AgNPs) was proposed to combat E. faecalis. The therapeutic effect of CD-AgNPs was investigated and a new cytocompatible Calcium Hydroxide-based endodontic sealer enriched with CD-AgNPs was synthesized that exhibited a steady release of Ag+ ions and lower water solubility at 24 hours, and enhanced antibacterial potential against E. faecalis. CD-AgNPs was synthesized and characterized morphologically and compositionally by Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy (FTIR), and UV-Vis Spectroscopy, followed by optimization via minimum inhibitory concentration (MIC) determination against E. faecalis by broth microdilution technique and Cytotoxicity analysis against NIH3T3 cell lines via Alamar Blue assay. Calcium hydroxide in distilled water was taken as control (C), Calcium hydroxide with to CD-AgNPs (5mg/ml and 10mg/ml) yielded novel endodontic sealers (E1 and E2). Morphological and chemical analysis of the novel sealers were done by SEM and FTIR; followed by in vitro assessment for antibacterial potential against E. faecalis via agar disc diffusion method, release of Ag+ ions for 21 days by Atomic Absorption Spectrophotometry and water solubility by weight change for 21 days. CD-AgNPs were 15–20 nm spherical-shaped particles in uniformly distributed clusters and revealed presence of constituent elements in nano-assembly. FTIR spectra revealed absorption peaks that correspond to various functional groups. UV-Vis absorption spectra showed prominent peaks that correspond to Carbon nanodots and Silver nanoparticles. CD-AgNPs exhibited MIC value of 5mg/ml and cytocompatibility of 84.47% with NIH3T3 cell lines. Novel endodontic sealer cut-discs revealed irregular, hexagonal particles (100–120 nm) with aggregation and rough structure with the presence of constituent elements. FTIR spectra of novel endodontic sealers revealed absorption peaks that correspond to various functional groups. Novel endodontic sealers exhibited enhanced antibacterial potential where E-2 showed greatest inhibition zone against E. faecalis (6.3±2 mm), a steady but highest release of Ag+ ions was exhibited by E-1 (0.043±0.0001 mg/mL) and showed water solubility of <3% at 24 hours where E-2 showed minimal weight loss at all time intervals. Novel endodontic sealers were cytocompatible and showed enhanced antibacterial potential against E. faecalis, however, E2 outperformed in this study in all aspects.

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Section: Discussionmentioning

confidence: 79%

Biochemical properties of novel Carbon nanodot-stabilized silver nanoparticles enriched calcium hydroxide endodontic sealer

Ali,

Gul,

Fareed

et al. 2024

PLoS ONE

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“…In a thermal decomposition reaction, high temperature breaks CaCO3 into calcium oxide (CaO; quicklime) and carbon dioxide. After hydration, the addition of water (H2O) to CaO, forms Ca(OH)2 [12,13]. Therefore, to further complement our previous study, the present study aimed to determine the antimicrobial property of Ca(OH)2 paste synthesized from natural limestone compared to conventional Ca(OH)2 paste as a control group.…”

Section: Introductionmentioning

confidence: 90%

“…Preparation of Ca(OH) 2 Paste. Ca(OH)2 powder from limestone was synthesized, as mentioned in our previous study [12,13]. The powder was prepared to a paste form with a composition described in Table 1 and placed in a sterile disposable 3cc syringe.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity of Calcium Hydroxide Synthesized from Indonesian Limestone against <i>Enterococcus faecalis</i>

Zakaria

Halimah

Sidiqa

et al. 2021

MSF

Self Cite

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Recurrent endodontic infections of treated teeth are mainly linked to microbial persistency in the root canal system. Enterococcus faecalis is a bacterium that is often identified in these recurrent infections. Calcium hydroxide (Ca(OH)2) is a widely used root canal medicament and is now being developed to be produced from natural materials. Ca(OH)2, which is synthesized from natural limestone from Palimanan, Indonesia, has a structure similar to Ca(OH)2, which is often used as a root canal medicament. The purpose of this study was to determine the antimicrobial property of Ca(OH)2 paste synthesized from natural limestone compared to conventional Ca(OH)2 paste. This study is an in vitro experimental study consists of 3 groups (n=24, namely, conventional Ca(OH)2 paste, limestone synthesized Ca(OH)2 paste, and saline as the negative control, for 2 intervals of time and evaluated by Total Plate Counting (TPC) method. Samples were mandibular first premolar root canal prepared by root canal preparation and contaminated with E. faecalis (ATCC 29212). The infected root canals of each group were treated by the medicaments, and samples from the root canals were taken on day 7 (n=4) and 14 (n=4). Results showed that both Ca(OH)2 groups had antimicrobial activities against E. faecalis. The limestone Ca(OH)2 paste group had the least amount of bacterial colonies on the 7th day compared to other groups. In contrast, on the 14th day, the conventional Ca(OH)2 paste had the least bacterial colonies. It can be concluded that the Ca(OH)2 paste synthesized from natural limestone has an antimicrobial effect on E. faecalis, the antimicrobial effect was higher on the 7th day but decreases on the 14th day, whereas the conventional Ca(OH)2 had a higher antimicrobial effect on day 14 compared to day 7. Further studies are expected to improve the long-term antimicrobial effectiveness and sustainability of the natural limestone synthesized Ca(OH)2 paste.

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Impact of Calcium Hydroxide Particle Size on the Intracanal Medicament Penetration Efficacy

Sidiqa,

Afidi,

Suntana

et al. 2024

DFMA

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Calcium hydroxide (Ca (OH)2) is an intracanal medicament used as a disinfectant in cases of tooth inflammation with ad dubia prognosis. The success of root canal treatment depends on the ability of intracanal medicaments to eliminate pathogenic bacteria present in the walls of narrow and complex root canals by releasing calcium and hydroxyl ions at the closest contacts. This study aimed to evaluate the effect of particle size on the ability to penetrate Ca (OH)2 in the coronal, middle, and apical root canals. Fifteen mandibular premolars extracted for orthodontic and periodontal purposes were collected and cut to produce root canals with a length of 12 cm. The root canals were then prepared with a Protaper SX-F3 needle and irrigated using a solution of 2.5% NaOCl, NaCl, and 17% EDTA as lubrication at each needle change. Ca (OH)2 with different particle sizes in paste form was manipulated with distilled water at a concentration of 0.8 g/mL then the paste was applied to the prepared tooth root canals and covered with a temporary filling. The samples were then stored in an incubator at 37 °C for 7 days. Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) was performed on the transverse surfaces of the coronal 1/3, middle 1/3 and apical 1/3 of the tooth. The maximum penetration depth was evaluated by measuring the maximum distance between the dentin canal wall and Ca (OH)2 present in the dentinal tubules. In all three zones, the Ca (OH)₂ nanoparticles had a greater penetration depth than the Ca (OH)₂ nanoparticles (P<0.001). In both groups, the penetration depth increased from the apical to the coronal section. All differences in the penetration test ability of the Ca (OH)₂ nanoparticles and Ca (OH)₂ microparticles at all depths of the surface. Ca (OH)₂ nanoparticles penetrate deeper into the dentinal tubules than Ca (OH)₂ microparticles do.

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Evaluation of calcium ion release in calcium hydroxide prototype as intracanal medicament

Cited by 4 publications

References 14 publications

Biochemical properties of novel Carbon nanodot-stabilized silver nanoparticles enriched calcium hydroxide endodontic sealer

Biochemical properties of novel Carbon nanodot-stabilized silver nanoparticles enriched calcium hydroxide endodontic sealer

Antimicrobial Activity of Calcium Hydroxide Synthesized from Indonesian Limestone against <i>Enterococcus faecalis</i>

Impact of Calcium Hydroxide Particle Size on the Intracanal Medicament Penetration Efficacy

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