Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Abstract

ObjectiveThe objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. Methods Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. ResultsThe diameters obtained to MTA (2.50 ± 0.00;2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) ResultadosOs diâmetros obtidos do MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), CP (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) e cimento de hidróxido de cálcio (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) foram expressos em milímetros e submetidos ao teste de Tukey (p<0.05). Conclusão METHODSThe tested materials were: MTA (Angelus Soluções Odontológicas, Londrina, Brasil), Portland cement (CP II -F32, ITAPESSOCA AGRO-INDUSTRIAL S.A., Goiana, Brasil) and calcium hydroxide paste (Reagen TM).The antimicrobial activity of the materials was evaluated by the Agar diffusion method against four different strains.The strains used for analysis were Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus and Lactobacillus casei isolated by the Laboratory of Microbiologic Science (Federal University of Viçosa -MG (UFV) and University State of Campinas -UNICAMP, SP, Brazil), respectively.Of each bacterial culture tested, it was held a concentration suspension of approximately 5x108 of colonies per mL, corresponding to 0.5 of Mc Farland em range of sterile medium Trypticase Soy Browth (TSBMerck). The Streptococcus mutans and Enterococcus faecalis suspensions were inoculated with spread plate in the middle of Sodium Tioglicolate (Merck) culture and Lactobacillus and Lactobacillus acidophilus casei were inoculated in the culture medium Man Rogosa & Sharpe Agar (MRS-HIMEDIA). Subsequently, the plates were incubated at 35 ± 1°C for 24/48h and the halos of inhibition were expressed in millimeters, using a caliper manual.A total of 12 plates were employed, each microorganism was tested in triplicate. Three cavities, measuring 4mm in diameter were made in each agar plate using a copper puncher and then completely filled with freshly manipulated product to be tested.Positive (CaOH) and negative controls (sterile growing medium) were tested using the same methodology and under the same conditions of the experiment.All cult...

Determination of the Free Radical Scavenging and Antimutagenic Activity of Mineral Trioxide Aggregate, Root Canal Treatment Material Used In Dentistry

“…It also has no reactive properties with any restorative material [24]. There are numerous MTA studies that evaluate antibacterial [25]; antimicrobial [26]; and antifungal [27] properties. Considering all those useful properties mentioned above; this study was carried out to evaluate the antimutagenic and antioxidant properties of MTA.…”