Evaluation of carrot resistance to alternaria leaf blight in controlled environments

Pawelec, Anna; Dubourg, C.; Briard, Mathilde

doi:10.1111/j.1365-3059.2006.01290.x

Cited by 39 publications

(36 citation statements)

References 9 publications

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“…Caporal) were used (Table 1). FRA001 and FRA017 were chosen as reference isolates because of their use in previous studies (Pawelec et al. , 2006; Boedo et al.…”

Section: Methodsmentioning

confidence: 99%

“…In previous papers focusing on different aspects of A. dauci – carrot interactions, such as infection processes, ALB resistance evaluation or genetic determinism (Dugdale et al. , 2000; Pawelec et al. , 2006; Boedo et al.…”

Section: Introductionmentioning

confidence: 99%

“…The first objective of the present study was to compare the aggressiveness of a set of A. dauci isolates after inoculation of a susceptible carrot cultivar which had previously been used as a positive disease control in several studies (Ben‐Noon et al. , 2001; Pawelec et al. , 2006; Boedo et al.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment

et al. 2011

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The aggressiveness of Alternaria dauci isolates was investigated in greenhouse conditions. Twenty-seven isolates were preselected from a large collection to represent high diversity according to geographic or host origins and intergenic spacer (IGS) polymorphism. IGS sequence analysis revealed that isolates were grouped within three different clusters. Eleven isolates were selected and inoculated on a susceptible carrot cultivar. Three criteria (mean lesion number, mean necrotic leaf area and mean disease index) were used to assess the aggressiveness of isolates. Continuous variation in aggressiveness was shown and no clear division into isolate classes was evident. For the host range study, two isolates were inoculated under greenhouse conditions onto nine cultivated Apiaceae species, two wild Daucus species and six cultivated non-Apiaceae species representing six botanical families. Lesions varying in severity were observed on all dicot species (Apiaceae and non-Apiaceae), but no symptoms developed on the two monocots studied (leek and sweetcorn). Plant species were also differentiated on the basis of expanding lesions (cultivated and wild carrot, dill and fennel) or non-expanding lesions (other dicot species). Typical A. dauci conidia were observed after in vitro incubation of leaves with symptoms. Fungal structures were isolated from lesions and A. dauci was confirmed on the basis of conidial morphology and specific conventional PCR results. Genotyping of individual isolates performed with microsatellite markers confirmed the presence of the inoculated isolate. The results clearly showed that, in controlled conditions, the host range of A. dauci is not restricted to carrot.

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“…Caporal) were used (Table 1). FRA001 and FRA017 were chosen as reference isolates because of their use in previous studies (Pawelec et al. , 2006; Boedo et al.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment

et al. 2011

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“…In addition to the usual approach for the evaluation of genotypes and new cultivars, we hypothesize that wheat (Triticum aestivum) cultivars with distinct levels of resistance to leaf rust (P. triticina) can be discriminated between by imaging chlorophyll fluorescence. Screening of genotypes under controlled conditions ensures the reproducibility of the timing of infection and minimizes to a large extent the possibility of simultaneous occurrence of other stresses (Huang et al 2005;Pawelec et al 2006). Furthermore, we studied the impact of inoculum density in a multi-temporal approach on the characteristic fluorescence readings of sensitive and resistant genotypes.…”

Section: Introductionmentioning

confidence: 99%

Quantum yield of non-regulated energy dissipation in PSII (Y(NO)) for early detection of leaf rust (Puccinia triticina) infection in susceptible and resistant wheat (Triticum aestivum L.) cultivars

2010

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The development and optimization of protocols for the precise and presymptomatic detection of diseases, and non-invasive evaluation of genotype-specific pathogen resistance enabling selection of the more promising genotypes in breeding programmes are important and often overlooked topics in precision agriculture. The increasing pressure to minimize both production costs and the environmental impact of pesticides forces the search for rapid and objective methods of screening pathogen resistance. Using the non-destructive pulse amplitude modulated (PAM) chlorophyll fluorescence imaging technique, we hypothesized that not only disease detection but also discrimination between differences in the level of resistance of wheat cultivars to the leaf rust (Puccinia triticina Erics.) pathogen can be achieved. Experiments were conducted using the cultivars Dekan and Retro as representatives of a susceptible and a highly resistant genotype, respectively. Fluorescence measurements were carried out daily on the control and on plants inoculated with P. triticina until the first small red-brown pustules appeared in the centre of chlorotic spots. In response to pathogen inoculation, the fluorescence readings showed an early characteristic increase in Y(NO) in both resistant and susceptible cultivars. The susceptible cultivar, however, showed a more pronounced difference between Y(NO) values measured on the control and inoculated leaves as well as a distinct evolution over time. Accordingly, our results indicate that Y(NO) might be suitable for discriminating between wheat genotypes as early as 2 days after inoculation. Thus, the proposed protocol might be adopted as an additional tool for the early screening of new genotypes, especially in breeding programs that aim for high resistance to disease and low crop variability for precision agriculture. However, its implementation in experimental field plots requires improvement of the measurement system and establishment of appropriate algorithms for disease pattern recognition and data analysis.

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“…For an objective, quantitative determination of disease impact (expressed as a percentage of affected leaf area), imaging techniques can significantly speed up throughput and ease quantification. Furthermore, screening under controlled conditions assures the reproducibility of the infection timing and excludes to a large extent the possibility of simultaneous occurrence of other stresses (Huang et al 2005;Pawelec et al 2006). Imaging does not only provide a way of digitising and quantifying visual damage, but can also reveal stress symptoms before that stage, by visualising the associated presymptomatic changes in photosynthesis and stomatal conductance.…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll fluorescence imaging for disease-resistance screening of sugar beet

Chaerle

Hagenbeek

Bruyne³

et al. 2007

Plant Cell Tiss Organ Cult

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Both biotic and abiotic stresses cause considerable crop yield losses worldwide (Chrispeels, Sadava Plants, genes, and crop biotechnology2003; Oerke, Dehne Crop Prot 23:275-285 2004). To speed up screening assays in stress resistance breeding, noncontact techniques such as chlorophyll fluorescence imaging can be advantageously used in the quantification of stress-inflicted damage. In comparison with visual spectrum images, chlorophyll fluorescence imaging reveals cell death with higher contrast and at earlier time-points. This technique has the potential to automatically quantify stress-inflicted damage during screening applications. From a physiological viewpoint, screening stress-responses using attached plant leaves is the ideal approach. However, leaf growth and circadian movements interfere with time-lapse monitoring of leaves, making it necessary to fix the leaves to be studied. From this viewpoint, a method to visualise the evolution of chlorophyll fluorescence from excised leaf pieces kept in closed petri dishes offers clear advantages. In this study, the plant-fungus interaction sugar beet-Cercospora beticola was assessed both in attached leaf and excised leaf strip assays. The attached leaf assay proved to be superior in revealing early, pre-visual symptoms and to better discriminate between the lines with different susceptibility to Cercospora.

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Evaluation of carrot resistance to alternaria leaf blight in controlled environments

Cited by 39 publications

References 9 publications

Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment

Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment

Quantum yield of non-regulated energy dissipation in PSII (Y(NO)) for early detection of leaf rust (Puccinia triticina) infection in susceptible and resistant wheat (Triticum aestivum L.) cultivars

Chlorophyll fluorescence imaging for disease-resistance screening of sugar beet

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