Evaluation of cartilage regeneration of chondrocyte encapsulated gellan gum-based hyaluronic acid blended hydrogel

Kim, Won Kyung; Choi, Joo Hee; Shin, Myeong Eun; Kim, Jin Woo; Kim, Pil Yun; Kim, Namyeong; Song, Jeong Eun; Khang, Gilson

doi:10.1016/j.ijbiomac.2019.08.176

Cited by 57 publications

(32 citation statements)

References 43 publications

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“…Kim et al [35] prepared chondrocyte encapsulated GG-based HA blended hydrogel for cartilage regeneration, and they reported that the hydrogel enhanced cell adhesion, viability, proliferation, and gene expression in an in vitro and in an in vivo model. The microstructure and morphology of the hydrogels are provided in Figure 3.…”

Section: Gellan Gum Cell Adhesion Propertiesmentioning

confidence: 99%

“…The mechanical properties of the GG are improved by combining it with inorganic materials (for their flexibility), and biopolymers (with poor rigidity) became common and smart solutions to improve the mechanical properties of GG. Composites of GG have been recently accomplished by the introduction of hydroxyapatite (HAp) [31], bioactive glass [32,33], calcium phosphate (CaP) [34], hyaluronic acid (HA) [35], demineralized bone powder (DBP) [36], polyethylene glycol [37], silk fibrin [38], agar [39], saponin [40], and chondroitin sulfate [41]. Table 1 shows the different types of GG composites that are used for biological applications.…”

Section: Introductionmentioning

confidence: 99%

“…Major routes for preparing gellan gum-based biomaterials[4,32,[34][35][36][37][38][39][65][66][67][68][69].…”

mentioning

confidence: 99%

“…SEM observation of hydrogel morphology, and 14 days of culturing chondrocyte cells on the scaffold surface[35].Molecules 2019, 24, x; doi:www.mdpi.com/journal/molecules…”

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confidence: 99%

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Biological Role of Gellan Gum in Improving Scaffold Drug Delivery, Cell Adhesion Properties for Tissue Engineering Applications

2019

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Over the past few decades, gellan gum (GG) has attracted substantial research interest in several fields including biomedical and clinical applications. The GG has highly versatile properties like easy bio-fabrication, tunable mechanical, cell adhesion, biocompatibility, biodegradability, drug delivery, and is easy to functionalize. These properties have put forth GG as a promising material in tissue engineering and regenerative medicine fields. Nevertheless, GG alone has poor mechanical strength, stability, and a high gelling temperature in physiological conditions. However, GG physiochemical properties can be enhanced by blending them with other polymers like chitosan, agar, sodium alginate, starch, cellulose, pullulan, polyvinyl chloride, xanthan gum, and other nanomaterials, like gold, silver, or composites. In this review article, we discuss the comprehensive overview and different strategies for the preparation of GG based biomaterial, hydrogels, and scaffolds for drug delivery, wound healing, antimicrobial activity, and cell adhesion. In addition, we have given special attention to tissue engineering applications of GG, which can be combined with another natural, synthetic polymers and nanoparticles, and other composites materials. Overall, this review article clearly presents a summary of the recent advances in research studies on GG for different biomedical applications.

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Section: Gellan Gum Cell Adhesion Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological Role of Gellan Gum in Improving Scaffold Drug Delivery, Cell Adhesion Properties for Tissue Engineering Applications

2019

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“…Nowadays natural/synthetic polymers have been proposed for the synthesis of biocompatible and bioactive substrates, also presenting high mechanical and physical properties that are needed for bone applications. The mainly used biomaterials include alginate, silk, collagen, chitosan, gellan gum, polylactide, polyglycolide, polydioxanone, and poly (lactic‐co‐glycolic acid) (Asti & Gioglio, ; W. K. Kim et al, ; Lutolf & Hubbell, ; Nair & Laurencin, ; Pankongadisak & Suwantong, ; Wang et al, ; X.‐y. Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Accelerating bone defects healing in calvarial defect model using 3D cultured bone marrow‐derived mesenchymal stem cells on demineralized bone particle scaffold

Kim

Park

Thangavelu

et al. 2020

J Tissue Eng Regen Med

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Bone defects are usually difficult to be regenerated due to pathological states or the size of the injury. Researchers are focusing on tissue engineering approaches in order to drive the regenerative events, using stem cells to regenerate bone. The purpose of this study is to evaluate the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) on biologically derived Gallus gallus domesticus-derived demineralized bone particle (GDD) sponge. The sponges were prepared by freezedrying method using 1, 2, and 3 wt% GDD and cross-linked with glutaraldehyde. The GDD sponge was characterized using scanning electron microscopy, compressive strength, porosity, and Fourier transform infrared. The potential bioactivity of the sponge was evaluated by osteogenic differentiation of BMSCs using 3(4, dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and quantifying alkaline phosphatase (ALP) activity. in vivo experiments were evaluated through a micro-computerized tomography (μ-CT) and histological assays. The analysis confirmed that an increase in the concentration of the GDD in the sponge leads to a higher bone formation and deposition in rat calvarial defects. Histological assay results were in line with μ-CT. The results reported in this study demonstrated the potential application of GDD sponges as osteoinductor in bone tissue engineering in pathological or nonunion bone defects. K E Y W O R D Sbone marrow-derived mesenchymal stem cells, bone defects, bone tissue engineering, demineralized bone particle, Gallus gallus domesticus, scaffold

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Natural Biopolymers for Tissue Engineering

Sarkar,

Ghosh,

Banerjee

et al. 2024

Biopolymers in Pharmaceutical and Food Applications

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Evaluation of cartilage regeneration of chondrocyte encapsulated gellan gum-based hyaluronic acid blended hydrogel

Cited by 57 publications

References 43 publications

Biological Role of Gellan Gum in Improving Scaffold Drug Delivery, Cell Adhesion Properties for Tissue Engineering Applications

Biological Role of Gellan Gum in Improving Scaffold Drug Delivery, Cell Adhesion Properties for Tissue Engineering Applications

Accelerating bone defects healing in calvarial defect model using 3D cultured bone marrow‐derived mesenchymal stem cells on demineralized bone particle scaffold

Natural Biopolymers for Tissue Engineering

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