Evaluation of CD307a expression patterns during normal B‐cell maturation and in B‐cell malignancies by flow cytometry

Auat, Mariángeles; Cardoso, Chandra Chiappin; Santos-Pirath, Íris Mattos; Rudolf-Oliveira, Renata Cristina Messores; Matiollo, Camila; Lange, Bárbara Gil; Silva, Jessica Pires da; Dametto, Gisele Cristina; Pirolli, Mayara Marin; Colombo, Maria; Santos-Silva, Maria Cláudia

doi:10.1002/cyto.b.21631

Cited by 6 publications

(2 citation statements)

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“…B cell Chronic Lymphocytic Leukemia (CLL) is a B‐cell lymphoproliferative disorder that is more prevalent in Western countries and in older people, with an incidence of 4.2:100.000/year . The diagnosis is made upon several criteria defined by clinical guidelines and it is distinguished from monoclonal B cell lymphocytosis (MBL) of CLL type, only by the number of pathological lymphocytes (>5,000/μL) and the presence of clinical symptoms: cytopenias, lymph node involvement, and organ damage . Among T cells, Vδ1 + T cells, have been pointed out as mediators in anti‐tumor responses performed by the host's immune system against CLL .…”

Section: Introductionmentioning

confidence: 99%

Quantification and phenotypic characterization of peripheral blood Vδ1 + T cells in chronic lymphocytic leukemia and monoclonal B cell lymphocytosis

Simões

Silva

Carvalho

et al. 2018

Cytometry Part B Clinical

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Background Vδ1+ T cells, a subset of γδ T cells, are responsible for innate‐like immune responses. Recently, an anti‐tumor function mediated by MHC‐unrestricted recognition of lipid and stress molecules, has also been described in these cells. This study aimed to quantify and phenotypically characterize circulating Vδ1+ T cells in B cell Chronic Lymphocytic Leukemia (CLL) and Monoclonal B cell lymphocytosis (MBL). Methods This study enrolled 58 individuals distributed in five groups: Binet B and C CLL (n = 9), Binet A CLL (n = 26), High count‐MBL (n = 10), Low count‐MBL (n = 5), and a control group (n = 8). The phenotypic characterization of Vδ1+ T cells, as well as the other T cell subpopulations (CD4+, CD8+, CD4+/CD8+, and Vδ1− γδT cells), were assessed by flow cytometry, evaluating the frequency of each subset expressing CD27, CD69, and cytoplasmic granzyme B. Results We observed an increasing percentage of Vδ1+ T cells belonging to CD27− compartment from controls to advanced stages of the disease, which was accompanied by an increasing percentage of these cells expressing granzyme B, a phenotypic pattern that was also observed in the other T cell subpopulations under study since earlier stages of the disease. Moreover, a striking expansion of Vδ1+ T cells in Binet B and C CLL was observed. Conclusions These experiment findings point to an expansion of CD27‐Vδ1+ T cells with a cytotoxic profile, from controls to advanced stages of the disease, which points to a role of Vδ1+ T cells in the host's anti‐tumor responses against clonal B‐cells in MBL and CLL. © 2018 Clinical Cytometry Society

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Section: Introductionmentioning

confidence: 99%

Quantification and phenotypic characterization of peripheral blood Vδ1 + T cells in chronic lymphocytic leukemia and monoclonal B cell lymphocytosis

Simões

Silva

Carvalho

et al. 2018

Cytometry Part B Clinical

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“…As far as Dr. Auat's paper is concerned, a flow cytometry measurement of CD307a antigen expression in B‐cell malignancies and during normal B‐cell maturation has been investigated with the main aim to explore the diagnostic role played by this molecule during normal and leukemic differentiation . The results indicate that the flow cytometry assessment of CD307a expression could be helpful to distinguish CLL from MCL, and the latter from MZL.…”

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confidence: 99%