Evaluation of cell proliferation in cystic lesions associated with impacted third molars

Abstract

The purpose of this study was to evaluate the metabolism and epithelial cell proliferation of odontogenic keratocyst (OKC), dentigerous cyst (DC), and unicystic ameloblastoma (UA) by quantifying the nucleolar organizing regions (AgNORs) and Ki-67 protein immunoexpression. Forty-eight cases (16 OKC, 16 DC, and 16 UA) were evaluated retrospectively. The metabolism and epithelial cell proliferation was measured by the Ki-67 positive cell percentage index and by the mean AgNOR count in each group. The Ki-67 and AgNOR counts were significantly higher in OKC comparing to the DC and UA (p < .001). Ki-67 positive cells were observed higher in suprabasal cell layers of OKC with uniform distribution, a few of them were predominantly observed in basal cell layer in DC and UA. The AgNOR count was significantly higher in the OKC basal cell layers and observed throughout the lining epithelium of DC and UA. Ki-67 and AgNOR reinforced the aggressive character of OKC, presenting high metabolism and cellular proliferation compared to DC and UA, possibly due to its more aggressive clinical behavior and high recurrence rate.Research Highlights 1. We evidence higher metabolism and epithelial cell proliferation in OKC when compared to UA and DC, supporting its aggressive aspect and its high rate of recurrence. OKC had intense and predominant labeling ofKi-67 on the suprabasal layer unlike UA and DC. K E Y W O R D S cell proliferation, metabolism, odontogenic cyst, impacted tooth, odontogenic tumors

STAT3 gene expression in ameloblastomas and odontogenic keratocysts

“…It is important to point out that, despite the lack of studies comparing STAT3 between different types of AM and OKC, there is no consensus in the literature regarding the distribution of tumor markers among different odontogenic cysts and tumors. However, this distribution is generally associated with proliferation, recurrence, and tumor aggressiveness [21,22], which cannot be inferred here.…”

Immunohistochemical Analysis of Dentigerous Cysts and Odontogenic Keratocysts Associated with Impacted Third Molars—A Systematic Review

“… MCM-3 is a more accurate marker for determining proliferation potential and is not influenced by inflammation, unlike Ki-67, making it more reliable for evaluating odontogenic cysts. Embaló et al (2018) [ 116 ] To evaluate the metabolism and epithelial cell proliferation of odontogenic keratocyst (OKC), dentigerous cyst (DC), and unicystic ameloblastoma (UA) by quantifying the nucleolar organizing regions (AgNORs) and Ki-67 protein immunoexpression. Study Type: Retrospective study.…”

A Incidência De Cistos Em Terceiros Molares Inclusos – Uma Revisão Integrativa

“…Os resíduos celulares epiteliais presentes no folículo dentário aderido à coroa do terceiro molar incluso podem viabilizar proliferações e diferenciações potencialmente patológicas, como o desenvolvimento de cistos odontogênicos, por exemplo (EMBALÓ et al, 2018;BASTOS et al, 2021). Lesões císticas são comumente associadas a terceiros molares impactados, com predileção mandibular (LIZIO et al, 2017;SUN et al, 2017;EMBALÓ et al, 2018). A presença de cistos na região posterior dos maxilares pode ser assintomática, provocar perdas e migrações dentais, bem como a fragilidade e reabsorção patológica do tecido ósseo adjacente (MELLO et al, 2019).…”