Evaluation of cellular attachment and proliferation on different surface charged functional cellulose electrospun nanofibers

Golizadeh, Mortaza; Karimi, Afzal; Gandomi‐Ravandi, Soheila; Vossoughi, Manouchehr; Khafaji, Mona; Joghataei, Mohammad Taghi; Faghihi, Faezeh

doi:10.1016/j.carbpol.2018.12.028

Cited by 68 publications

(24 citation statements)

References 33 publications

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“…Nowadays, with the rise of nanotechnology approaches, cellulose nanofibers have been engineered by electrospinning in the form of nanocomposite wound dressings that not only protect the wounds, but they are capable of releasing drugs that inhibit post-operative adhesions, stimulate hemodialysis and hemostasis, and repair tissue defects [20]. Cellulose electrospun nanofibers have been studied due to their ultrafine and highly porous structure, biocompatibility, biodegradability, hydrophilicity, low density, thermostability, low thermal expansion, and easy chemical modification [20,84]. Even though research continues in this field, the disappointing mechanical properties and the difficulties in processing cellulose in the form of nanofibers via electrospinning remain very important challenges.…”

Section: Cellulosementioning

confidence: 99%

“…Cellulose, being one of the most abundant natural polymers on Earth, with relatively easy extraction, superior biocompatibility, non-toxicity, and biodegradability, has been considered as a factual option for wound dressings formulations, either as an additive or as base substrate. Acquired data has been very promising, with excellent effects being registered in regard to cell adhesion and growth [20,21]. However, the production of natural cellulose-based nanofibers, regenerated cellulose nanofibers, and even microfibers via electrospinning remains a very challenging process, due to their inability to dissolve in water and common organic solvents.…”

Section: Introductionmentioning

confidence: 99%

“…However, the production of natural cellulose-based nanofibers, regenerated cellulose nanofibers, and even microfibers via electrospinning remains a very challenging process, due to their inability to dissolve in water and common organic solvents. In fact, most of the cellulose-containing nanofibers have been produced after extensive tests with a variety of solvents or by combining cellulose with other materials, e.g., polymers, metals or ceramics, and loading those formulations with bioactive molecules, such as drugs and growth factors [20,22]. The introduction of chemical groups within the structure of cellulose has facilitated processing and contributed for the emergence of cellulose derivatives, like cellulose acetate (CA), which is the most common derivative that is considered by the European Committee for Standardization (CEN) as a bio-based polymer [23].…”

Section: Introductionmentioning

confidence: 99%

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Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

et al. 2020

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Wound healing requires careful, directed, and effective therapies to prevent infections and accelerate tissue regeneration. In light of these demands, active biomolecules with antibacterial properties and/or healing capacities have been functionalized onto nanostructured polymeric dressings and their synergistic effect examined. In this work, various antibiotics, nanoparticles, and natural extract-derived products that were used in association with electrospun nanocomposites containing cellulose, cellulose acetate and different types of nanocellulose (cellulose nanocrystals, cellulose nanofibrils, and bacterial cellulose) have been reviewed. Renewable, natural-origin compounds are gaining more relevance each day as potential alternatives to synthetic materials, since the former undesirable footprints in biomedicine, the environment, and the ecosystems are reaching concerning levels. Therefore, cellulose and its derivatives have been the object of numerous biomedical studies, in which their biocompatibility, biodegradability, and, most importantly, sustainability and abundance, have been determinant. A complete overview of the recently produced cellulose-containing nanofibrous meshes for wound healing applications was provided. Moreover, the current challenges that are faced by cellulose acetate- and nanocellulose-containing wound dressing formulations, processed by electrospinning, were also enumerated.

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Section: Cellulosementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

et al. 2020

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“…To measure swelling behavior, dry weight (Wd) of each sample was recorded, after immersion in water, the sample weight was measured again to record its wet weight (Ww). The water uptake ability of the samples was obtained by Equation (1) [24]:…”

Section: Characterization Of Multifunctional Nanofibers and Microfibersmentioning

confidence: 99%

Fabrication of multifunctional microfibrous and nanofibrous cellulose carriers and comparison of cell adhesion and spreading potential on them

2020

Biointerface Res Appl Chem

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Fibrous biomaterials have received much attention in tissue engineering and regenerative medicine due to their morphology, resembling extracellular matrix. In comparison to synthetic fibers, cellulose based fibers have interesting properties for cellular applications such as biodegradability, biocompatibility, simple preparation and their potential for chemical modification. Among cellulose derivatives, carboxymethyl cellulose and quaternized cellulose are the most important and valuable cellulose ethers which have anionic and cationic surface charge. In this research, we report the fabrication of multifunctional cellulose microfibrous and nanofibrous scaffolds and the comparison of adhesion and spreading potential of human fibroblast cell on them. The fabricated fibrous scaffolds were characterized by several instrumental techniques. The results showed that multifunctional cellulose nanofibers and microfiber had 8.6 and 8.2 mV surface potential, 7.1 and 6.8 MPa tensile strength, 560 and 510 MPa Young modules, 610 and 595% water uptake and 41o and 44o contact angle, respectively. The MTT assay showed that proliferation of fibroblast cells was enhanced in nanofibrous, compared to microfibrous mat. The SEM analysis of fixed cells on scaffolds showed that cells spreading on nanofibrous samples became more noticeable than microfibrous ones.

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“…Covering the surface of Fe3O4 nanoparticles could be a novel approach to circumvent the mentioned drawbacks [24]. Carboxymethyl cellulose (CMC) has a great potential to play a linkage role, and it is also a natural polymer with biocompatibility and good environmental stability [25,26]. Therefore, CMC was selected for coating the Fe3O4 nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Laccase from Trametes hirsuta onto CMC Coated Magnetic Nanoparticles

Sadeghzadeh

Ghazvini

Hejazi

et al. 2020

IJE

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In this study, Fe3O4/CMC magnetic nanoparticles were synthesized through co-precipitation method. Afterward, laccase from Trametes hirsuta was immobilized onto Carboxymethyl cellulose (CMC)coated magnetic Fe3O4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. Also, the resulted magnetic nanoparticles and immobilized laccase were characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis. Moreover, the vital factors in enzyme immobilization, such as contact time, amount of N-hydroxysuccinimide (NHS), and the amount of nanoparticles were optimized, which successively 48 h, 0.01 g, and 0.0125 g were achieved for 0.01g of N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide (EDC). Activity recovery of 51 ± 0.8% was achieved by optimizing the immobilization process. The results also indicated that the loading of laccase onto carboxymethyl cellulose-coated Fe3O4 nanoparticles was approximately 120 (mg/g). Finally, the immobilized laccases on magnetic support could save nearly 50% of their initial activity after five consecutive cycles.

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Evaluation of cellular attachment and proliferation on different surface charged functional cellulose electrospun nanofibers

Cited by 68 publications

References 33 publications

Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

Fabrication of multifunctional microfibrous and nanofibrous cellulose carriers and comparison of cell adhesion and spreading potential on them

Immobilization of Laccase from Trametes hirsuta onto CMC Coated Magnetic Nanoparticles

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