Evaluation of Cellulases Production by Aspergillus niger Using Response Surface Methodology

Zohri, Abd El-Naser; Ali, Mahmoud; Moamen, sara

doi:10.21608/esugj.2022.155979.1017

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…A. niger plays a crucial role in the fermentation of S. alba by producing enzymes such as cellulase, which can degrade plant cell walls (Zohri et al 2022). This enzymatic activity breaks down components of organic matter that are difficult to digest into substrates more easily accessed and utilized by rumen microbes.…”

Section: Organic Matter Digestibilitymentioning

confidence: 99%

Optimizing Nutrient Digestibility Through Fermentation of Mangrove (Sonneratia alba) Fruit with Aspergillus niger: Implications for Livestock Feed Quality Improvement

2024

Int J Vet Sci

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Utilizing mangrove (Sonneratia alba) fruit as an alternative feed source presents a viable avenue for enhancing livestock feed efficiency and sustainability. However, the intrinsic high tannin content of S. alba poses a significant barrier to its digestibility and utility as a feed component. The application of fermentation processes facilitated by Aspergillus niger emerges as a strategic intervention to diminish tannin impediments and elevate the feed material's nutritional profile. This investigation delves into the ramifications of varied fermentation periods with A. niger on the digestibility, nutrient quality, and fermentative by-products of S. alba fruit. Adopting a completely randomized block design, the study administers treatments spanning fermentation durations of 7, 10, 13, and 16 days, scrutinizing their influence on a spectrum of digestibility indices, ruminal fluid properties, microbial protein synthesis, and gaseous production. The findings articulate that protracted fermentation markedly augments the digestibility of dry matter, organic matter, and crude protein, alongside elevations in volatile fatty acids and ammonia levels, while sparing rumen pH and overall gas output from significant alterations. Notably, a 16-day fermentation tenure culminates in optimal feed digestibility and nutritional amelioration, underscoring the potency of extended A. niger fermentation in curtailing tannin contents and fostering the adaptability of S. alba fruit as livestock feed, thereby advocating for advanced, sustainable feed formulation methodologies.

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Section: Organic Matter Digestibilitymentioning

confidence: 99%

Optimizing Nutrient Digestibility Through Fermentation of Mangrove (Sonneratia alba) Fruit with Aspergillus niger: Implications for Livestock Feed Quality Improvement

2024

Int J Vet Sci

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“…The lignocellulolytic index on table 2 demonstrated that KB2 and KB3 isolate had moderate potentials as a lignocellulolytic hydrolyzing isolate (clear zones of 29.3 mm and 49.3 mm) while the cellulolytic indexes are 1.19 and 1.57 respectively. A study on the cellulase production of various isolates of Aspergillus niger also resulted in cellulolytic indexes (hydrolysis capacity/HC) ranging from 1.11 to 1.32 (Zohri et al, 2023). Various studies indicated that the majority of Aspergillus, Fusarium, Alternaria, Rhizopus, Penicillum and Trichoderma isolates were found to possess cellulolytic activity (Vieto et al, 2021;Vasiliauskienė et al, 2023;Pacheco et al, 2023).…”

Section: Cellulolytic Activitymentioning

confidence: 99%

Isolation and Molecular Screening of Fungus as Agents in Cellulolytic Transformation Materials from Symbiotic Lichen

Rahardiyan,

Moko

2023

J Bio Bio Edu

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The abundance of lignocellulosic waste makes it a potential source for advanced biomaterials through various transformation processes. Lignocellulosic biomass transformation to advanced biomaterials involves enzymes from extracellular metabolites of microorganisms capable of hydrolyzing lignocellulose. This research was to molecularly screen fungi found in symbiotes of lichen endemic to trees growing in North Minahasa (North Sulawesi) with lignocellulolytic transformation enzymes. Molecular screening was conducted from identified fungi isolates based on partial genetical analysis on the locus of Internal Transcribed Spacer (ITS) of the fungi’s ribosomal DNA. Fungi isolates screening identified Trichoderma koningiopsis (isolate HZA8 and isolate HZA6), Penicillium sumatraense (strain CBS 127365 and strain CBS 130380), Trichoderma hamatum (isolate PAN12-45 and isolate PAN12-05), Aspergillus aculeatus (strain A1.9 18S), Aspergillus aculeatus (isolate XSD-74), Trichoderma reesei (strain S2606 and isolate 5A14). Molecular identification and BLAST homology of potentially lignocellulolytic fungi isolates rDNA indicated that isolate KB2 had close relationship with Trichoderma reesei at 100% degree of closeness and an index of cellulolytic activity of 1.19. While isolate KB3 appeared closely related to Aspergillus aculeatus at 99.83% degree of closeness and an index of cellulolytic activity of 1.57. Therefore, the potential of developing bioprocess industries in general and in particular is most probable.

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Unveiling Fungal Proficiency in Microplastic Degradation: A Comprehensive Research Investigation

ARIF,

Hassan,

Fakhry

et al. 2024

Preprint

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Microplastics (MPs) are prevalent pollutants in environments that are colonized by various groups of microbes. Fungi are considered among the most efficient microbial degraders of MPs because they produce salient enzymes and can survive on recalcitrant compounds with limited nutrients. While most studies have focused on the occurrence of MPs in wastewater treatment systems, MP degradation in fresh water and wastewater is generally poorly understood. Therefore, the current study included the isolation of some genera of fungi from the Tigris River water environment that have the ability to degrade MPs in both natural and artificial environments utilizing synthetic media. Using weight loss measurements, Fourier transform infrared spectroscopy (FTIR) was used to identify the chemical structure of the plastic polymers, and scanning electron microscopy (SEM) was used to determine the size and morphology of the microplastics and the degree of plastic consumed by the aquatic fungus. The biodegradation of high-density polyethylene (HDPE) and polystyrene (PS) by the aquatic fungus Aspergillus carbonarius and Eurotium sp. was also examined. Overall, Aspergillus carbonarius and Eurotium sp. were able to degrade HDPE more efficiently than PS without requiring any prior microplastic treatment. Therefore, the ability of fungi to degrade MPs was confirmed by weight loss, FTIR, and SEM data. Therefore, the results indicate that the isolated fungus has a promising future for polymer breakdown in both artificial and natural environments. Investigating the long-term impacts and gaining a deeper knowledge of the mechanisms of microplastic disintegration should be the main goals of future research.

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Evaluation of Cellulases Production by Aspergillus niger Using Response Surface Methodology

Cited by 3 publications

References 23 publications

Optimizing Nutrient Digestibility Through Fermentation of Mangrove (Sonneratia alba) Fruit with Aspergillus niger: Implications for Livestock Feed Quality Improvement

Optimizing Nutrient Digestibility Through Fermentation of Mangrove (Sonneratia alba) Fruit with Aspergillus niger: Implications for Livestock Feed Quality Improvement

Isolation and Molecular Screening of Fungus as Agents in Cellulolytic Transformation Materials from Symbiotic Lichen

Unveiling Fungal Proficiency in Microplastic Degradation: A Comprehensive Research Investigation

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