Evaluation of Cellulolytic and Hemicellulolytic Abilities of Fungi Isolated from Coffee Residue and Sawdust Composts

Eida, Mohamed F.; Nagaoka, Toshinori; Wasaki, Jun; Kouno, Kenji

doi:10.1264/jsme2.me10210

Cited by 26 publications

(21 citation statements)

References 52 publications

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“…This finding may indicate that the enzyme profile of microorganisms is not only genus and species-specific, but also isolate-specific. This speculation is supported by the result of our previous study, in which we also found that the enzyme profiles of cellulolytic and hemicellulolytic fungi were isolate-specific (11). …”

Section: Discussionsupporting

confidence: 77%

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

et al. 2012

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Clarifying the identity and enzymatic activities of microorganisms associated with the decomposition of organic materials is expected to contribute to the evaluation and improvement of composting processes. In this study, we examined the cellulolytic and hemicellulolytic abilities of bacteria isolated from sawdust compost (SDC) and coffee residue compost (CRC). Cellulolytic bacteria were isolated using Dubos mineral salt agar containing azurine cross-linked (AZCL) HE-cellulose. Bacterial identification was performed based on the sequence analysis of 16S rRNA genes, and cellulase, xylanase, β-glucanase, mannanase, and protease activities were characterized using insoluble AZCL-linked substrates. Eleven isolates were obtained from SDC and 10 isolates from CRC. DNA analysis indicated that the isolates from SDC and CRC belonged to the genera Streptomyces, Microbispora, and Paenibacillus, and the genera Streptomyces, Microbispora, and Cohnella, respectively. Microbispora was the most dominant genus in both compost types. All isolates, with the exception of two isolates lacking mannanase activity, showed cellulase, xylanase, β-glucanase, and mannanase activities. Based on enzyme activities expressed as the ratio of hydrolysis zone diameter to colony diameter, it was suggested that the species of Microbispora (SDCB8, SDCB9) and Paenibacillus (SDCB10, SDCB11) in SDC and Microbispora (CRCB2, CRCB6) and Cohnella (CRCB9, CRCB10) in CRC contribute to efficient cellulolytic and hemicellulolytic processes during composting.

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Section: Discussionsupporting

confidence: 77%

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

et al. 2012

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“…Similar to Anastasi et al [4], Acremonium and Cladosporium occurred in both windrow and vermicompost, and Scytalidium was more abundant in windrow than vermicompost. It is no surprise that Arthrographis and Galactomyces occur in compost given their ability to produce cellulolytic enzymes [56,57], however, this is the first report for composts made by ASP and vermicompost processes, specifically.…”

Section: Dynamics Of Composting Processmentioning

confidence: 92%

Microbial Anaerobic Digestion (Bio-Digesters) as an Approach to the Decontamination of Animal Wastes in Pollution Control and the Generation of Renewable Energy

2015

Biological Treatment of Solid Waste

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Compost production is a critical component of organic waste handling, and compost applications to soil are increasingly important to crop production. However, we know surprisingly little about the microbial communities involved in the composting process and the factors shaping compost microbial dynamics. Here, we used high-throughput sequencing approaches to assess the diversity and composition of both bacterial and fungal communities in compost produced at a commercial-scale. Bacterial and fungal communities responded to both compost recipe and composting method. Specifically, bacterial communities in manure and hay recipes contained greater relative abundances of Firmicutes than hardwood recipes with hay recipes containing relatively more Actinobacteria and Gemmatimonadetes. In contrast, hardwood recipes contained a large relative abundance of Acidobacteria and Chloroflexi. Fungal communities of compost from a mixture of dairy manure and silage-based bedding were distinguished by a greater relative abundance of Pezizomycetes and Microascales. Hay recipes uniquely contained abundant Epicoccum, Thermomyces, Eurotium, Arthrobotrys, and Myriococcum. Hardwood recipes contained relatively abundant Sordariomycetes. Holding recipe constant, there were significantly different bacterial and fungal communities when the composting process was managed by windrow, aerated static pile, or vermicompost. Temporal dynamics of the composting process followed known patterns of degradative succession in herbivore manure. The initial community was dominated by Phycomycetes, followed by Ascomycota and finally Basidiomycota. Zygomycota were associated more with manure-silage and hay than hardwood composts. Most commercial composters focus on the thermophilic phase as an economic means to insure sanitation of compost from pathogens. However, the community succeeding the thermophilic phase begs further investigation to determine how the microbial dynamics observed here can be best managed to generate compost with the desired properties.

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“…Trichoderma belongs to fungi known for their ability to degrade cellulose [76]. The presence of antagonistic fungi in composted sawdust was also reported by Eida et al [77]. Fungi listed as antagonists are also known as metal-tolerant filamentous fungi.…”

Section: Mutual Relations Between Populations Of Potentially Phytopatmentioning

confidence: 75%

Composts and Water Extracts of Lignocellulosic Composts in the Aspect of Fertilization, Humus-Forming, Sanitary, Phytosanitary and Phytotoxicity Value Assessment

Bohacz

2018

Waste Biomass Valor

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Purpose Lignocellulosic biomass waste is produced in large quantities and only a small part is used for utility purposes. The aim of the work was to assess the fertilization value of the solid fraction and water extract of lignocellulosic composts. Methods Compost I contained 42.86% pine bark, 34.28% grass, 20.0% sawdust and 2.86% chicken feathers; compost II contained 25.54% pine bark, 10.63% wheat straw, 51.07% sawdust and 12.76% chicken feathers. Seldom described physical properties and phytosanitary and sanitary status of composts as well as macro-and micronutrients and heavy metals concentrations were determined in the solid fraction and water extracts of composts and their impact on the germination of Lepidium sativum L. using PC analysis. Results Composts were characterized by good physical properties and a light product, safe phytosanitary status, which was related to the prevalence of potentially antagonistic fungi, especially in compost II. The frequency of Aspergillus fumigatus, considered an opportunistic human pathogen, decreased during the composting. Heavy metal concentrations did not exceed permissible values for improvers and cultivation media in the solid and aqueous compost fractions. The tested composts were sanitary safe. Both composts were non-toxic to plants, because the germination index in compost I was PCA analysis demonstrated that the majority of micro-and macronutrients and heavy metals had a significantly negative effect on root elongation and germination of the test plant (cress) seeds, especially in compost I. Conclusions Lignocellulosic waste after composting is a valuable and safe organic fertilizer.

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Evaluation of Cellulolytic and Hemicellulolytic Abilities of Fungi Isolated from Coffee Residue and Sawdust Composts

Cited by 26 publications

References 52 publications

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

Microbial Anaerobic Digestion (Bio-Digesters) as an Approach to the Decontamination of Animal Wastes in Pollution Control and the Generation of Renewable Energy

Composts and Water Extracts of Lignocellulosic Composts in the Aspect of Fertilization, Humus-Forming, Sanitary, Phytosanitary and Phytotoxicity Value Assessment

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