Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2

Alam, Imranul; Asahina, Izumi; Ohmamiuda, Kohichi; Takahashi, Kouichiro; Yokota, Shoji; Enomoto, Shoji

doi:10.1016/s0142-9612(00)00322-7

Cited by 122 publications

(46 citation statements)

References 25 publications

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“…This work builds on our previous efforts establishing that incorporation of inorganic materials such as TCP, HAp, and bioactive glass (BG) alone in microsphere-based scaffolds provides both bioactive cues and building blocks for osteogenic differentiation of cells [33, 27, 24, 35, 28]. Additionally, it has been shown by others that combining TCP and HAp for bone regeneration is a promising strategy given that a combination of these materials provides both osteoinductive and osteoconductive cues to the surrounding cells [30, 41, 42, 31, 43–46]. Furthermore, we have previously demonstrated that higher concentrations (10 and 20 wt%) of TCP and HAp in microsphere-based scaffolds were more favorable for synthesis of ECM components relevant to bone tissue, thus providing the motivation for encapsulating even higher concentrations of TCP and HAp than our previous reported studies [33, 27].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, differences in gene expression were observed between the TCP/HAp encapsulating groups with both the groups showing fluctuating expressions for RUNX2, COL1A1, and BGLAP over time. These oscillating expression patterns in the TCP/HAp groups may suggest that the rBMSCs responded differently to different TCP/HAp ratios in microsphere-based scaffolds [54, 30, 41, 31, 43]. …”

Section: Discussionmentioning

confidence: 99%

“…Homogenous microsphere-based scaffolds were fabricated using PLGA microspheres encapsulating TCP and HAp mixtures in two of the most widely studied w/w ratios of 7:3 and 1:1 (TCP:HAp) with the same net ceramic content of 30 wt% [29–31]. The response of rBMSCs to the bioceramic mixtures was evaluated when cultured in a medium consisting of exogenous factors.…”

Section: Introductionmentioning

confidence: 99%

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Microsphere-based scaffolds encapsulating tricalcium phosphate and hydroxyapatite for bone regeneration

Gupta

Lyne

Barragan

et al. 2016

J Mater Sci: Mater Med

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Bioceramic mixtures of tricalcium phosphate (TCP) and hydroxyapatite (HAp) are widely used for bone regeneration because of their excellent cytocompatibility, osteoconduction, and osteoinduction. Therefore, we hypothesized that incorporation of a mixture of TCP and HAp in microsphere-based scaffolds would enhance osteogenesis of rat bone marrow stromal cells (rBMSCs) compared to a positive control of scaffolds with encapsulated bone-morphogenic protein-2 (BMP-2). Poly(D,L-lactic-co-glycolic acid) (PLGA) microsphere-based scaffolds encapsulating TCP and HAp mixtures in two different ratios (7:3 and 1:1) were fabricated with the same net ceramic content (30 wt%) to evaluate how incorporation of these ceramic mixtures would affect the osteogenesis in rBMSCs. Encapsulation of TCP/HAp mixtures impacted microsphere morphologies and the compressive moduli of the scaffolds. Additionally, TCP/HAp mixtures enhanced the end-point secretion of extracellular matrix (ECM) components relevant to bone tissue compared to the “blank” (PLGA-only) microsphere-based scaffolds as evidenced by the biochemical, gene expression, histology, and immunohistochemical characterization. Moreover, the TCP/HAp mixture groups even surpassed the BMP-2 positive control group in some instances in terms of matrix synthesis and gene expression. Lastly, gene expression data suggested that the rBMSCs responded differently to different TCP/HAp ratios presented to them. Altogether, it can be concluded that TCP/HAp mixtures stimulated the differentiation of rBMSCs toward an osteoblastic phenotype, and therefore may be beneficial in gradient microsphere-based scaffolds for osteochondral regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microsphere-based scaffolds encapsulating tricalcium phosphate and hydroxyapatite for bone regeneration

Gupta

Lyne

Barragan

et al. 2016

J Mater Sci: Mater Med

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“…Another publication by the same group [27] in which the pellets were implanted into a subcutaneous pocket instead of the cranium produced conflicting results and a higher degree of hydroxyapatite was recommended. The main difference between these two studies was the site of implantation.…”

Section: Discussionmentioning

confidence: 99%

Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC

Geiger

Beverungen

Lorenz

et al. 2012

JFB

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VEGF (vascular endothelial growth factor) promotes vascularization and remodeling of bone substitutes. The aim of this study was to examine the effect of distinct resorbable ceramic carriers on bone forming capacities of VEGF transfected bone marrow stromal cells (BMSC). A critical size defect of the radius in rabbits was filled either by a low surface scaffold called beta-TCP (tricalciumphsphate) or the high surface scaffold CDHA (calcium deficient hydroxy-apatite) loaded with autologous BMSC, which were either transfected with a control plasmid or a plasmid coding for phVEGF165. They were compared to unloaded scaffolds. Thus, six treatment groups (n = 6 in each group) were followed by X-ray over 16 weeks. After probe retrieval, the volume of new bone was measured by micro-CT scans and vascularization was assessed in histology. While only minor bone formation was found in both carriers when implanted alone, BMSC led to increased osteogenesis in both carriers. VEGF promoted vascularization of the scaffolds significantly in contrast to BMSC alone. Bone formation was increased in the beta-TCP group, whereas it was inhibited in the CDHA group that showed faster scaffold degradation. The results indicate that the interaction of VEGF transfected BMSC with resorbable ceramic carrier influences the ability to promote bone healing.

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“…After the suspension was centrifuged for 15 min at 3000 g, the supernatant liquid was obtained. 33) The optical density in an aliquot of the supernatant was measured at 500 nm with a spectrophotometer.…”

Section: Characterization Of Porous Calcium Phosphatesmentioning

confidence: 99%

泡セラミックス法と水熱処理法を組み合わせた多孔質リン酸カルシウムの合成とその細胞応答性

Aoki

Yamaguchi

Nakahira

et al. 2004

J. Ceram. Soc. Japan

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AZ~bNX@AEM@ðgÝí¹½½E¿_JVEÌ¬AE »Ì×E«ÂØTîERûrYE½ ÖÞ EÀº åãåwYAEÈw¤C5670047 ïØsüäu 81 Þ sH|@ÛåwH|w¨¿HwÈC6068585 ss ¶ae¼PèäC¹¬Four porous calcium phosphates (HAp, HAp/bTCP, bTCP, and aTCP) were prepared using a ceramic foaming technique combined with a hydrothermal treatment. These porous calcium phosphates had enough pore size, high specific surface area, and high porosity (80÷) for the cell growth of osteoblastlike cells. The compositions of the porous calcium phosphates depended on the solution in hydrothermal treatment and the sintering temperature. The cell activities of MC3T3E1 cells on porous HAp, HAp/bTCP, and bTCP were similar to that of monolayer cultivation as a control in MTT assay, ALPase activities, and the cell density.

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Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2

Cited by 122 publications

References 25 publications

Microsphere-based scaffolds encapsulating tricalcium phosphate and hydroxyapatite for bone regeneration

Microsphere-based scaffolds encapsulating tricalcium phosphate and hydroxyapatite for bone regeneration

Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC

泡セラミックス法と水熱処理法を組み合わせた多孔質リン酸カルシウムの合成とその細胞応答性

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