In order to implement the Mechanistic-Empirical Pavement Design Guide (MEPDG) to design and maintain asphalt pavements in China, it is necessary to calibrate transfer functions of distresses in MEPDG with local conditions, including traffics, environment, and materials as well as measured pavement distresses data in field. Comprehensive single factor sensitivity analyses of factors that influence thermal cracking of asphalt pavements were conducted utilizing the MEPDG low temperature cracking (LTC) model. Additionally, multiple factor sensitivity analyses were carried out as well, based on which pavement structures with sound thermal cracking resistance were recommended for seasonal frozen regions in China. Finally, the field data of thermal cracks on typical asphalt pavements in China was utilized to calibrate the LTC model in MEPDG. An improvement was proposed on MEPDG LTC model, after which was applied, the predicted thermal cracking from MEPDG LTC model agrees well with measured thermal cracking in China.