Evaluation of Chemical Composition of Hamadan Propolis as a Potential Anticancer Agent

Dastan, Dara; Mahmoudi, Roghayeh; Saidijam, Massoud; Khoei, Saeideh Gholamzadeh; Abdali, Maede Hasan; Afshar, Saeid

doi:10.5812/jjcmb.115348

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…Recently, Fang, Xiong, Xu, Yin, and Luo [ 37 ] reported the proapoptotic activity of polyphenolic compounds such as ferulic acid and caffeic acid on human tongue squamous carcinoma cells (CAL-27). Current results were higher than the findings of Dastan et al [ 22 ] who reported propolis methanolic extracts with ( IC 50 ) 702.5 and 177.7 μ g mL −1 after 24 and 48 hours.…”

Section: Discussioncontrasting

confidence: 87%

“…The propolis extract (P1) collected from Ziarat region expressed maximum flavonoid content (0.6586 ± 0.329) and the extract (P6) from Bela region have the lowest content (0.1546 ± 0.087) mg of quercetin g −1 of propolis extract presented in ( Table 2 ). Results of current study are minimum than other reported studies from different location in Turkey [ 3 ], Pakistan [ 1 ], China [ 21 ], and Iran [ 22 ]. According to Choi et al [ 20 ], the variations in total phenolic and total flavonoids of propolis samples depend on their geographic origin.…”

Section: Discussioncontrasting

confidence: 84%

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Bio-Functional Potential and Biochemical Properties of Propolis Collected from Different Regions of Balochistan Province of Pakistan

Akbar

Gul

Aziz

et al. 2022

Oxidative Medicine and Cellular Longevity

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Propolis is a well-known resinous natural substance collected by honeybees (Apis mellifera L.) from plants exudations. Variations in chemical composition of propolis are due to different sources from which it is collected and change in climate and geographical location. In this study, different propolis samples were collected from different regions of Balochistan and examined for its chemical composition, total phenolics and total flavonoid contents, and antioxidant potential by using DPPH radical scavenging assay and antimicrobial activity. Bioactive components analysis revealed the presence of steroids, carbohydrates, flavonoids, coumarins, cardiac glycosides, quinones, anthraquinones, terpenoids, tannins, and phlobatannins at different levels. The total phenolics contents were ranged from 2.9343 ± 1.247 to 6.0216 ± 2.873 mg GAE g-1, and flavonoid contents were found to be 0.1546 ± 0.087 to 0.6586 ± 0.329 mg QE g-1, respectively. The antioxidant ability of each extract was analyzed by their concentration having 50% inhibition ( I C 50 ). The propolis sample P3 possessed lower I C 50 27.07 ± 0.73 mg mL−1 with higher % inhibition of DPPH radical, and P8 showed lower % inhibition by having I C 50 84.43 ± 2.07 mg mL−1. The antibacterial activity of all samples was analyzed against a wide group of bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumonia and propolis extract (P4) was highly active against Klebsiella pneumoniae with the maximum diameter of zone of inhibition 20.33 ± 1.52 mm, and propolis extract (P3) showed maximum zone of inhibition against Escherichia coli 19.06 ± 1.90 , while propolis extract (P2) was found less active with minimum diameter of zone of inhibition 7.46 ± 1.50 mm . The antifungal activity of extract was considered as active against the fungal species. Propolis extract (P3) showed 82% of zone of inhibition against Aspergillus Niger, and propolis extract (P1) was highly active against Aspergillus parasiticus with 80% of zone of inhibition. By comparing the vibration frequencies in wave numbers of the sample spectrograph acquired from an FTIR spectrophotometer, the functional groups present in the extracts were identified. The presence of seven elements (Fe, Zn, Mn, Ni, Pb, Cd, and Cr) was analyzed through atomic absorption spectrophotometer. The obtained concentrations were within the permissible ranges established by the World Health Organization. The GC-MS analysis revealed the presence of 80 different compounds belonged to different classes. The obtained results confirmed the imperative potential of propolis which can be used in various biological applications.

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Section: Discussioncontrasting

confidence: 87%

Section: Discussioncontrasting

confidence: 84%

Bio-Functional Potential and Biochemical Properties of Propolis Collected from Different Regions of Balochistan Province of Pakistan

Akbar

Gul

Aziz

et al. 2022

Oxidative Medicine and Cellular Longevity

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Functional Potential and Chemical Profile Analysis of Propolis Oil Extracted from Propolis of Balochistan

Aziz

Akbar

Gul

et al. 2022

Journal of Food Quality

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Propolis oil (PO) was examined for chemical composition, phenolic and flavonoid content, and antioxidant and antimicrobial potential. Phenolic and flavonoid contents were 2.388 ± 1.116 mg GAE/g and 0.579 ± 0.140 mg QE/g. Oil showed 64.59 ± 14.59% inhibition of DPPH radical and significant antibacterial activities against target bacteria. Salmonella typhi was found to be highly sensitive (27.23 ± 4.35 mm) to PO, compared to Escherichia coli (23.40 ± 3.21), Staphylococcus aureus (21.43 ± 2.80), and Klebsiella pneumoniae (21.26 ± 3.25). The MIC and MBS values of PO were 0.35 and 0.7 mg/mL for S. typhi and E. coli, whereas they were 0.7 and 1.4 mg/mL for S. aureus. Moreover, the PO was found to be bacteriostatic for K. pneumoniae. Aspergillus flavus was found to be highly sensitive to PO, with an effective growth inhibition percentage of 73%, followed by Aspergillus niger (70%), whereas Aspergillus parasiticus was less sensitive with 25% growth inhibition. Functional groups in PO were determined with an FTIR spectrophotometer, and alcohol, alkane, aldehydes, alkenes, and ketones groups were found to be present, whereas GC-MS analysis revealed the presence of 27 different medicinal compounds, among which α-copanene (29.85%), benzyl benzoate (26.8%), 2,4-bis[1-(4-hydroxyphenyl)isopropyl]phenol, acetophenone (14.92%), undecylenic aldehyde (7.46%), p-linalool (5.9%), and ethyl 3-phenylpropionate (4.47%) were found in abundance.

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The protective effect of DMI on hippocampus EEG, behavioral and biochemical parameters in hypoxia-induced seizure on neonatal period

Nazarizadeh,

Ghotbeddin,

Ghafouri

et al. 2024

PLoS ONE

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Hypoxia-Induced Neonatal Seizure (HINS) is a prevalent type of seizure in infants caused by hypoxic conditions, which can lead to an increased risk of epilepsy, learning disabilities, and cognitive impairments later in life. This study focuses on examining the effects of dimethyl itaconate (DMI) on cognition, motor coordination, and anxiety-like behavior in male rats that have experienced HINS. 42 male Wistar newborn rats (PND10) were randomly divided into six groups (n = 7). 1) Control (Vehicle only); received DMI solvent (0.1ml) without applying hypoxia. 2–3) DMI; receiving (20 and 50 mg/kg; i.p). 4) HINS; they were placed in a hypoxia chamber with 7% oxygen and 93% nitrogen concentration for 15 minutes. 5–6) DMI+HINS; received DMI (20 and 50 mg/kg; i.p) 24h before hypoxia. Behavioral tests including; Novel object recognition test, Rotarod, Parallel bar, Open field and elevated plus maze (EPM); started at age 45 after birth. After behavioral tests, the hippocampal CA1 region local EEG was recorded in all groups. Then the brain hippocampus tissue was isolated and the amount of MDA, SOD, NO, and Thiol was measured by ELISA method. Data showed that the administration of DMI improved motor symptoms, anxiety-like behaviors, and cognition in HINS rats (p<0.05). EEG power in the HINS group decreased significantly compared to other experimental groups (p<0.05). Biochemical observations showed that DMI significantly reduced oxidative stress and inflammation in the hippocampal tissue of HINS rats (p<0.05). Increased hippocampal oxidative stress and inflammation can be effective in the occurrence of behavioral disorders observed in HINS rats. While DMI improved these behavioral impairments by reducing oxidative stress and inflammation.

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Evaluation of Chemical Composition of Hamadan Propolis as a Potential Anticancer Agent

Cited by 4 publications

References 42 publications

Bio-Functional Potential and Biochemical Properties of Propolis Collected from Different Regions of Balochistan Province of Pakistan

Bio-Functional Potential and Biochemical Properties of Propolis Collected from Different Regions of Balochistan Province of Pakistan

Functional Potential and Chemical Profile Analysis of Propolis Oil Extracted from Propolis of Balochistan

The protective effect of DMI on hippocampus EEG, behavioral and biochemical parameters in hypoxia-induced seizure on neonatal period

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